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Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration

Pure iron (Fe) and its alloys have been recently emphasized as potential biodegradable metals due to their good mechanical properties that are close to those of stainless steel 316L. This research was focused more on the study of cell-material interaction and to analyze the effect of corrosion produ...

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Main Author: Mohd. Daud, Nurizzati
Format: Thesis
Language:English
Published: 2014
Subjects:
QH301 Biology
Online Access:http://eprints.utm.my/id/eprint/77992/1/NurizzatiMohdDaudMFBME20141.pdf
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spelling my.utm.779922018-07-18T07:50:01Z http://eprints.utm.my/id/eprint/77992/ Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration Mohd. Daud, Nurizzati QH301 Biology Pure iron (Fe) and its alloys have been recently emphasized as potential biodegradable metals due to their good mechanical properties that are close to those of stainless steel 316L. This research was focused more on the study of cell-material interaction and to analyze the effect of corrosion product on cell behavior by performing degradation study. In this study, samples were prepared by coating hydroxyapatite (HA) and hydroxyapatite/poly (s-Caprolactone) (HA/PCL) onto porous iron using dip coating method. Biosafety and biofunctionality of the sample were evaluated by using human skin fibroblast (HSF) and mesenchymal stem (MSC) cells. Analysis by Inductively Coupled Plasma- Mass Spectrometry (ICP-MS) revealed that concentration of ion Fe was decreased in the medium containing HAcoated Fe. However, the weight loss of the sample is high compared to pure porous iron and HA/PCL-coated Fe. A positive cell response to the Fe ions was revealed during the first 21 days of the cell toxicity study using indirect method. After 21 days the HSF cell viability decreased due to acidic eluates and the increase of Fe ions concentration that promoted the formation of the reactive oxygen intermediates (ROI). From the results obtained, it showed that the HSF and MSC cells exhibited higher viability when in contact with the Fe-HA and Fe-PCL/HA than with the Fe specimens. However, there is a significant decrease (p<0.05) of cells when cultured on three different samples after 3 days of incubation. HA-coated porous Fe also provides support for attachment of the cells. Observation under Scanning Electron Microscope (SEM) reveals that the filopodia of the mesenchymal stem cells preferred to develop onto irregular surface of HA-coated Fe. This study provided evidences of a good cell-material interaction on the porous Fe that may confirm the feasibility of using porous biodegradable ferum as hard tissue scaffolds. 2014-03 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/77992/1/NurizzatiMohdDaudMFBME20141.pdf Mohd. Daud, Nurizzati (2014) Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration. Masters thesis, Universiti Teknologi Malaysia, Faculty of Built Environment. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:90311
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Mohd. Daud, Nurizzati
Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
description Pure iron (Fe) and its alloys have been recently emphasized as potential biodegradable metals due to their good mechanical properties that are close to those of stainless steel 316L. This research was focused more on the study of cell-material interaction and to analyze the effect of corrosion product on cell behavior by performing degradation study. In this study, samples were prepared by coating hydroxyapatite (HA) and hydroxyapatite/poly (s-Caprolactone) (HA/PCL) onto porous iron using dip coating method. Biosafety and biofunctionality of the sample were evaluated by using human skin fibroblast (HSF) and mesenchymal stem (MSC) cells. Analysis by Inductively Coupled Plasma- Mass Spectrometry (ICP-MS) revealed that concentration of ion Fe was decreased in the medium containing HAcoated Fe. However, the weight loss of the sample is high compared to pure porous iron and HA/PCL-coated Fe. A positive cell response to the Fe ions was revealed during the first 21 days of the cell toxicity study using indirect method. After 21 days the HSF cell viability decreased due to acidic eluates and the increase of Fe ions concentration that promoted the formation of the reactive oxygen intermediates (ROI). From the results obtained, it showed that the HSF and MSC cells exhibited higher viability when in contact with the Fe-HA and Fe-PCL/HA than with the Fe specimens. However, there is a significant decrease (p<0.05) of cells when cultured on three different samples after 3 days of incubation. HA-coated porous Fe also provides support for attachment of the cells. Observation under Scanning Electron Microscope (SEM) reveals that the filopodia of the mesenchymal stem cells preferred to develop onto irregular surface of HA-coated Fe. This study provided evidences of a good cell-material interaction on the porous Fe that may confirm the feasibility of using porous biodegradable ferum as hard tissue scaffolds.
format Thesis
author Mohd. Daud, Nurizzati
author_facet Mohd. Daud, Nurizzati
author_sort Mohd. Daud, Nurizzati
title Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_short Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_full Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_fullStr Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_full_unstemmed Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_sort biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
publishDate 2014
url http://eprints.utm.my/id/eprint/77992/1/NurizzatiMohdDaudMFBME20141.pdf
http://eprints.utm.my/id/eprint/77992/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:90311
_version_ 1643657695785385984
score 13.211869

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