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Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina

The present study was carried out to optimize the physical and nutritional parameters for cultivation of Trichoderma harzianum Rifai for production of extracellular beta-glucosidase, an alternative to chemcial fungicides to control Macrophomina phaseolina, which causes charcoal rot disease in crops....

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Main Authors: Khalili, Elham, Huyop, Fahrul, Manan, Fatin Myra Abd, Wahab, Roswanira Abdul
Format: Article
Language:English
Published: Taylor and Francis Ltd. 2017
Subjects:
QH Natural history
Online Access:http://eprints.utm.my/id/eprint/77526/1/ElhamKhalili2017_OptimizationofCultivationConditions%20inBananaWastes.pdf
http://eprints.utm.my/id/eprint/77526/
https://www.tandfonline.com/doi/full/10.1080/13102818.2017.1342562
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spelling my.utm.775262018-09-27T04:14:13Z http://eprints.utm.my/id/eprint/77526/ Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina Khalili, Elham Huyop, Fahrul Manan, Fatin Myra Abd Wahab, Roswanira Abdul QH Natural history The present study was carried out to optimize the physical and nutritional parameters for cultivation of Trichoderma harzianum Rifai for production of extracellular beta-glucosidase, an alternative to chemcial fungicides to control Macrophomina phaseolina, which causes charcoal rot disease in crops. Response surface methodology by the Box-Behnken design (BBD) based on four factors (temperature, carbon sources, inoculum size and pH) was used for optimization of the cultivation conditions to produce beta-glucosidase using agro-industrial banana wastes. The highest -glucosidase activity (1483.27U/mL) was attained under optimized cultivation conditions: approximate to 36 degrees C and pH 7.3, using carbon source and inoculum size of 10% (w/v) and 5% (w/v), respectively. It is noteworthy that the low deviation values (0.09-0.44%) in the verification experiments (R1, R2 and R3) inferred the generated model was accurate to predict optimal cultivation conditions of T. harzianum Rifai. Likewise, the obtained diameters of inhibition zones ranging from 58.00-38.66mm following treatment with beta-glucosidase was found comparable to other in vitro tests using pure T. harzianum isolates and chemical fungicides. Hence, the findings indicated that it was feasible to use beta-glucosidase as a greener alternative to chemical fungicides for control of M. phaseolina infection and consequently, for protection of crops against charcoal rot disease. Taylor and Francis Ltd. 2017 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/77526/1/ElhamKhalili2017_OptimizationofCultivationConditions%20inBananaWastes.pdf Khalili, Elham and Huyop, Fahrul and Manan, Fatin Myra Abd and Wahab, Roswanira Abdul (2017) Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina. Biotechnology and Biotechnological Equipment, 31 (5). pp. 921-934. ISSN 1310-2818 https://www.tandfonline.com/doi/full/10.1080/13102818.2017.1342562
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QH Natural history
spellingShingle QH Natural history
Khalili, Elham
Huyop, Fahrul
Manan, Fatin Myra Abd
Wahab, Roswanira Abdul
Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
description The present study was carried out to optimize the physical and nutritional parameters for cultivation of Trichoderma harzianum Rifai for production of extracellular beta-glucosidase, an alternative to chemcial fungicides to control Macrophomina phaseolina, which causes charcoal rot disease in crops. Response surface methodology by the Box-Behnken design (BBD) based on four factors (temperature, carbon sources, inoculum size and pH) was used for optimization of the cultivation conditions to produce beta-glucosidase using agro-industrial banana wastes. The highest -glucosidase activity (1483.27U/mL) was attained under optimized cultivation conditions: approximate to 36 degrees C and pH 7.3, using carbon source and inoculum size of 10% (w/v) and 5% (w/v), respectively. It is noteworthy that the low deviation values (0.09-0.44%) in the verification experiments (R1, R2 and R3) inferred the generated model was accurate to predict optimal cultivation conditions of T. harzianum Rifai. Likewise, the obtained diameters of inhibition zones ranging from 58.00-38.66mm following treatment with beta-glucosidase was found comparable to other in vitro tests using pure T. harzianum isolates and chemical fungicides. Hence, the findings indicated that it was feasible to use beta-glucosidase as a greener alternative to chemical fungicides for control of M. phaseolina infection and consequently, for protection of crops against charcoal rot disease.
format Article
author Khalili, Elham
Huyop, Fahrul
Manan, Fatin Myra Abd
Wahab, Roswanira Abdul
author_facet Khalili, Elham
Huyop, Fahrul
Manan, Fatin Myra Abd
Wahab, Roswanira Abdul
author_sort Khalili, Elham
title Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_short Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_full Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_fullStr Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_full_unstemmed Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_sort optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by trichoderma harzianum rifai efficient for in vitro inhibition of macrophomina phaseolina
publisher Taylor and Francis Ltd.
publishDate 2017
url http://eprints.utm.my/id/eprint/77526/1/ElhamKhalili2017_OptimizationofCultivationConditions%20inBananaWastes.pdf
http://eprints.utm.my/id/eprint/77526/
https://www.tandfonline.com/doi/full/10.1080/13102818.2017.1342562
_version_ 1643657567580192768
score 13.211869

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