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Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1

Background/Purpose: Currently, silver nanoparticles ()AgNPs) have gained importance in various industrial applications. However, their impact upon release into the environment on microorganisms remains unclear. The aim of this study was to analyze the effect of polyvinylpyrrolidone-capped AgNPs synt...

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Main Authors: Lau, Chew Ping, Abdul Wahab, Mohd. Firdaus, Jaafar, Jafariah, Chan, Giek Far, Rashid, Noor Aini Abdul
Format: Article
Language:English
Published: Elsevier Taiwan 2017
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/77342/1/LauChewPing2017_ToxicEffectofHighConcentrationofSonochemically.pdf
http://eprints.utm.my/id/eprint/77342/
http://dx.doi.org/10.1016/j.jmii.2015.08.004
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spelling my.utm.773422019-01-28T04:45:23Z http://eprints.utm.my/id/eprint/77342/ Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1 Lau, Chew Ping Abdul Wahab, Mohd. Firdaus Jaafar, Jafariah Chan, Giek Far Rashid, Noor Aini Abdul Q Science (General) Background/Purpose: Currently, silver nanoparticles ()AgNPs) have gained importance in various industrial applications. However, their impact upon release into the environment on microorganisms remains unclear. The aim of this study was to analyze the effect of polyvinylpyrrolidone-capped AgNPs synthesized in this laboratory on two bacterial strains isolated from the environment, Gram-negative Citrobacter sp. A1 and Gram-positive Enterococcus sp. C1. Methods: Polyvinylpyrrolidone-capped AgNPs were synthesized by ultrasound-assisted chemical reduction. Characterization of the AgNPs involved UVevisible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and energy dispersive X-ray spectroscopy. Citrobacter sp. A1 and Enterococcus sp. C1 were exposed to varying concentrations of AgNPs, and cell viability was determined. Scanning electron microscopy was performed to evaluate the morphological alteration of both species upon exposure to AgNPs at 1000 mg/L. Results: The synthesized AgNPs were spherical in shape, with an average particle size of 15 nm. The AgNPs had different but prominent effects on either Citrobacter sp. A1 or Enterococcus sp. C1. At an AgNP concentration of 1000 mg/L, Citrobacter sp. A1 retained viability for 6 hours, while Enterococcus sp. C1 retained viability only for 3 hours. Citrobacter sp. A1 appeared to be more resistant to AgNPs than Enterococcus sp. C1. The cell wall of both strains was found to be morphologically altered at that concentration. Conclusion: Minute and spherical AgNPs significantly affected the viability of the two bacterial strains selected from the environment. Enterococcus sp. C1 was more vulnerable to AgNPs, probably due to its cell wall architecture and the absence of silver resistance-related genes. Copyright | 2015, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. Elsevier Taiwan 2017-08 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/77342/1/LauChewPing2017_ToxicEffectofHighConcentrationofSonochemically.pdf Lau, Chew Ping and Abdul Wahab, Mohd. Firdaus and Jaafar, Jafariah and Chan, Giek Far and Rashid, Noor Aini Abdul (2017) Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1. Journal Of Microbiology Immunology And Infection, 50 (4). pp. 427-434. ISSN 1684-1182 http://dx.doi.org/10.1016/j.jmii.2015.08.004 DOI:10.1016/j.jmii.2015.08.004
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Lau, Chew Ping
Abdul Wahab, Mohd. Firdaus
Jaafar, Jafariah
Chan, Giek Far
Rashid, Noor Aini Abdul
Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1
description Background/Purpose: Currently, silver nanoparticles ()AgNPs) have gained importance in various industrial applications. However, their impact upon release into the environment on microorganisms remains unclear. The aim of this study was to analyze the effect of polyvinylpyrrolidone-capped AgNPs synthesized in this laboratory on two bacterial strains isolated from the environment, Gram-negative Citrobacter sp. A1 and Gram-positive Enterococcus sp. C1. Methods: Polyvinylpyrrolidone-capped AgNPs were synthesized by ultrasound-assisted chemical reduction. Characterization of the AgNPs involved UVevisible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and energy dispersive X-ray spectroscopy. Citrobacter sp. A1 and Enterococcus sp. C1 were exposed to varying concentrations of AgNPs, and cell viability was determined. Scanning electron microscopy was performed to evaluate the morphological alteration of both species upon exposure to AgNPs at 1000 mg/L. Results: The synthesized AgNPs were spherical in shape, with an average particle size of 15 nm. The AgNPs had different but prominent effects on either Citrobacter sp. A1 or Enterococcus sp. C1. At an AgNP concentration of 1000 mg/L, Citrobacter sp. A1 retained viability for 6 hours, while Enterococcus sp. C1 retained viability only for 3 hours. Citrobacter sp. A1 appeared to be more resistant to AgNPs than Enterococcus sp. C1. The cell wall of both strains was found to be morphologically altered at that concentration. Conclusion: Minute and spherical AgNPs significantly affected the viability of the two bacterial strains selected from the environment. Enterococcus sp. C1 was more vulnerable to AgNPs, probably due to its cell wall architecture and the absence of silver resistance-related genes. Copyright | 2015, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.
format Article
author Lau, Chew Ping
Abdul Wahab, Mohd. Firdaus
Jaafar, Jafariah
Chan, Giek Far
Rashid, Noor Aini Abdul
author_facet Lau, Chew Ping
Abdul Wahab, Mohd. Firdaus
Jaafar, Jafariah
Chan, Giek Far
Rashid, Noor Aini Abdul
author_sort Lau, Chew Ping
title Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1
title_short Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1
title_full Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1
title_fullStr Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1
title_full_unstemmed Toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on Citrobacter sp.A1 and Enterococcus sp.C1
title_sort toxic effect of high concentration of sonochemically synthesized polyvinylpyrrolidone-coated silver nanoparticles on citrobacter sp.a1 and enterococcus sp.c1
publisher Elsevier Taiwan
publishDate 2017
url http://eprints.utm.my/id/eprint/77342/1/LauChewPing2017_ToxicEffectofHighConcentrationofSonochemically.pdf
http://eprints.utm.my/id/eprint/77342/
http://dx.doi.org/10.1016/j.jmii.2015.08.004
_version_ 1643657550111965184
score 13.211869

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