Cover Image

Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography

Simultaneous enantioseparation of three chiral triazole fungicides i.e. penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) under acidic condition was investigated with different HP-?-CD concentrations as chiral selector. The three tria...

Full description

Saved in:
Bibliographic Details
Main Authors: Wan Ibrahim, Wan Aini, Hermawan, Dadan, Sanagi, Mohd. Marsin
Format: Article
Language:English
Published: Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia 2008
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/7705/1/WanAinWan008_EnantioseparationofPenconazole.pdf
http://eprints.utm.my/id/eprint/7705/
http://jfs.ibnusina.utm.my/index.php/jfs/article/view/79
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.7705
record_format eprints
spelling my.utm.77052010-10-08T09:46:57Z http://eprints.utm.my/id/eprint/7705/ Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography Wan Ibrahim, Wan Aini Hermawan, Dadan Sanagi, Mohd. Marsin QD Chemistry Simultaneous enantioseparation of three chiral triazole fungicides i.e. penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) under acidic condition was investigated with different HP-?-CD concentrations as chiral selector. The three triazole fungicides have an asymmetrically substituted carbon atom respectively, and they all contain two enantiomers. In order to achieve the best enantioresolution of these triazole fungicides, the HP-?-CD concentration range was optimized from 0 to 40 mM in 10 mM increment. The results indicate that separation of enantiomers was not achieved with concentration range of HP-?-CD from 0 to 20 mM. Simultaneous enantioseparation was successfully achived at 30 mM and 40 mM HP-?-CD concentration in 25 mM phosphate buffer (pH 3.0) solution that containing 50 mM SDS, 10% methanol and 5% acetonitrile. However, enantioresolution decreased for myclobutanil and triadimefon enantiomers with HP-?-CD concentration greater than 30 mM. In addition, an increase in the concentration of HP-?-CD caused a significant increase in the analysis time. In light of these aspects, the optimal concentration for HP-?-CD was decided to be 30 mM for the simultaneous chiral separation of the three triazole fungicides with resolutions (Rs) between enantiomers for penconazole, Rs = 0.81; myclobutanil, Rs = 1.14; triadimefon, Rs = 2.09; peak efficiencies (N) greater than 108 000 for all stereoisomers and analysis time of less than 15 min Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia 2008 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/7705/1/WanAinWan008_EnantioseparationofPenconazole.pdf Wan Ibrahim, Wan Aini and Hermawan, Dadan and Sanagi, Mohd. Marsin (2008) Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography. Journal of Fundamental Sciences, 4 (2). pp. 445-450. ISSN 1823-626X http://jfs.ibnusina.utm.my/index.php/jfs/article/view/79
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Wan Ibrahim, Wan Aini
Hermawan, Dadan
Sanagi, Mohd. Marsin
Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography
description Simultaneous enantioseparation of three chiral triazole fungicides i.e. penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) under acidic condition was investigated with different HP-?-CD concentrations as chiral selector. The three triazole fungicides have an asymmetrically substituted carbon atom respectively, and they all contain two enantiomers. In order to achieve the best enantioresolution of these triazole fungicides, the HP-?-CD concentration range was optimized from 0 to 40 mM in 10 mM increment. The results indicate that separation of enantiomers was not achieved with concentration range of HP-?-CD from 0 to 20 mM. Simultaneous enantioseparation was successfully achived at 30 mM and 40 mM HP-?-CD concentration in 25 mM phosphate buffer (pH 3.0) solution that containing 50 mM SDS, 10% methanol and 5% acetonitrile. However, enantioresolution decreased for myclobutanil and triadimefon enantiomers with HP-?-CD concentration greater than 30 mM. In addition, an increase in the concentration of HP-?-CD caused a significant increase in the analysis time. In light of these aspects, the optimal concentration for HP-?-CD was decided to be 30 mM for the simultaneous chiral separation of the three triazole fungicides with resolutions (Rs) between enantiomers for penconazole, Rs = 0.81; myclobutanil, Rs = 1.14; triadimefon, Rs = 2.09; peak efficiencies (N) greater than 108 000 for all stereoisomers and analysis time of less than 15 min
format Article
author Wan Ibrahim, Wan Aini
Hermawan, Dadan
Sanagi, Mohd. Marsin
author_facet Wan Ibrahim, Wan Aini
Hermawan, Dadan
Sanagi, Mohd. Marsin
author_sort Wan Ibrahim, Wan Aini
title Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography
title_short Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography
title_full Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography
title_fullStr Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography
title_full_unstemmed Enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography
title_sort enantioseparation of penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography
publisher Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia
publishDate 2008
url http://eprints.utm.my/id/eprint/7705/1/WanAinWan008_EnantioseparationofPenconazole.pdf
http://eprints.utm.my/id/eprint/7705/
http://jfs.ibnusina.utm.my/index.php/jfs/article/view/79
_version_ 1643644834592849920
score 13.211869

Similar Items