職員瀏覽: Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment

Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment

Sonication decellularization treatment ability to efficiently decellularize meniscus tissues requires proper evaluations. The purpose of this study is to investigate the maintenance of biomechanical properties within meniscus scaffolds. In sonication decellularization treatment, tissue samples were...

全面介紹

Saved in:

書目詳細資料
Main Authors:	Norzarini, A., Kitajima, T., Feng, Z., Sha'ban, M., Azhim, A.
格式:	Article
出版:	American Scientific Publishers 2017
主題:	T Technology (General)
在線閱讀:	http://eprints.utm.my/id/eprint/76434/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009909829&doi=10.1166%2fjbt.2017.1565&partnerID=40&md5=e9c025a8b9e34a899144791640d915c3
標簽:	添加標簽沒有標簽, 成為第一個標記此記錄!

id	my.utm.76434
record_format	eprints
spelling	my.utm.764342018-04-30T13:23:53Z http://eprints.utm.my/id/eprint/76434/ Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment Norzarini, A. Kitajima, T. Feng, Z. Sha'ban, M. Azhim, A. T Technology (General) Sonication decellularization treatment ability to efficiently decellularize meniscus tissues requires proper evaluations. The purpose of this study is to investigate the maintenance of biomechanical properties within meniscus scaffolds. In sonication decellularization treatment, tissue samples were sonicated at 40 kHz ultrasound frequency in 0.1% sodium dodecyl sulfate (SDS) for 10 h. For control, an immersion treatment by 0.1% SDS was applied in the absence of ultrasonic exposure. Cell removal efficiency was determined through a histological analysis of van Gieson staining. The treatment effects on viscoelastic properties that are crucial for scaffolds strength were properly studied. Extracellular matrix (ECM) properties were determined through Picrosirius red and Safranin-O/Fast green staining. The decellularized tissues were further evaluated by scanning electron microscopy (SEM) for its ultrastructure. The findings demonstrated a complete nuclei removal in sonication-treated tissues compared to immersion-treated tissues. Viscoelastic properties, namely stiffness, compression, and residual force were maintained after the sonication decellularization treatment. The maintenance of viscoelastic properties was supported by ECM preservation that is mainly composed of collagen and GAG contents. An ultrastructure observation showed the presence of micropores on the surface of sonication-treated tissues. The micropores are determined as chondrocytes lacunae that conserve chondrocytes within it. Sonication decellularization treatment is thus capable of preparing the meniscus scaffolds candidate in which the biomechanical strength of tissues is maintained by preserving the ECM properties. American Scientific Publishers 2017 Article PeerReviewed Norzarini, A. and Kitajima, T. and Feng, Z. and Sha'ban, M. and Azhim, A. (2017) Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment. Journal of Biomaterials and Tissue Engineering, 7 (3). pp. 223-232. ISSN 2157-9083 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009909829&doi=10.1166%2fjbt.2017.1565&partnerID=40&md5=e9c025a8b9e34a899144791640d915c3 DOI:10.1166/jbt.2017.1565
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	T Technology (General)
spellingShingle	T Technology (General) Norzarini, A. Kitajima, T. Feng, Z. Sha'ban, M. Azhim, A. Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment
description	Sonication decellularization treatment ability to efficiently decellularize meniscus tissues requires proper evaluations. The purpose of this study is to investigate the maintenance of biomechanical properties within meniscus scaffolds. In sonication decellularization treatment, tissue samples were sonicated at 40 kHz ultrasound frequency in 0.1% sodium dodecyl sulfate (SDS) for 10 h. For control, an immersion treatment by 0.1% SDS was applied in the absence of ultrasonic exposure. Cell removal efficiency was determined through a histological analysis of van Gieson staining. The treatment effects on viscoelastic properties that are crucial for scaffolds strength were properly studied. Extracellular matrix (ECM) properties were determined through Picrosirius red and Safranin-O/Fast green staining. The decellularized tissues were further evaluated by scanning electron microscopy (SEM) for its ultrastructure. The findings demonstrated a complete nuclei removal in sonication-treated tissues compared to immersion-treated tissues. Viscoelastic properties, namely stiffness, compression, and residual force were maintained after the sonication decellularization treatment. The maintenance of viscoelastic properties was supported by ECM preservation that is mainly composed of collagen and GAG contents. An ultrastructure observation showed the presence of micropores on the surface of sonication-treated tissues. The micropores are determined as chondrocytes lacunae that conserve chondrocytes within it. Sonication decellularization treatment is thus capable of preparing the meniscus scaffolds candidate in which the biomechanical strength of tissues is maintained by preserving the ECM properties.
format	Article
author	Norzarini, A. Kitajima, T. Feng, Z. Sha'ban, M. Azhim, A.
author_facet	Norzarini, A. Kitajima, T. Feng, Z. Sha'ban, M. Azhim, A.
author_sort	Norzarini, A.
title	Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment
title_short	Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment
title_full	Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment
title_fullStr	Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment
title_full_unstemmed	Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment
title_sort	characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment
publisher	American Scientific Publishers
publishDate	2017
url	http://eprints.utm.my/id/eprint/76434/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009909829&doi=10.1166%2fjbt.2017.1565&partnerID=40&md5=e9c025a8b9e34a899144791640d915c3
_version_	1643657309980721152
score	13.251813

Characterization based on biomechanical properties for meniscus scaffolds by sonication decellularization treatment

相似書籍