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Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)

Bioassay-guided isolation was used to investigate the antioxidants and α-glucosidase inhibitors from extract of root of Cassia sieberiana. The ethyl acetate fraction demonstrated strong free radical scavenging (DPPH and ABTS+) and potent α-glucosidase inhibition. The subsequent fractionation and pur...

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Main Authors: Jibril, S., Sirat, H. M., Basar, N.
Format: Article
Language:English
Published: ACG Publications 2017
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/75725/1/HasnahMohdSirat_Bioassay-GuidedIsolationofAntioxidantsanda-Glucosidase.pdf
http://eprints.utm.my/id/eprint/75725/
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spelling my.utm.757252018-04-30T13:12:03Z http://eprints.utm.my/id/eprint/75725/ Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae) Jibril, S. Sirat, H. M. Basar, N. QD Chemistry Bioassay-guided isolation was used to investigate the antioxidants and α-glucosidase inhibitors from extract of root of Cassia sieberiana. The ethyl acetate fraction demonstrated strong free radical scavenging (DPPH and ABTS+) and potent α-glucosidase inhibition. The subsequent fractionation and purification of the ethyl acetate fraction using silica gel chromatography and Sephadex LH-20, yielded; islandicin (1), chrysophanol (2), physcion (3), emodin (4), quercetin (5), kaempferol (6), dihydrokaempferol (7), and piceatannol (8). Quercetin (5) showed the most active antioxidant activity with IC50 values of 1.58 mM and 1.30 mM against DPPH and ABTS+ radicals, followed by piceatannol (8) with IC50 values of 3.96 mM and 3.28 mM, which is better than the standard BHT (with IC50 value 8.93 mM) and trolox (with IC50 value 8.25 mM), for DPPH and ABTS+ radicals scavenging activities, respectively. For the α-glucosidase inhibitory assay, quercetin (5) and piceatannol (8) showed higher potency against α-glucosidase with IC50 values of 5.73 μM and 7.37 μM respectively, than standard quercetin with IC50 value of 9.20 μM and acarbose with IC50 value of 14.12 μM. This study presents the first report on the α-glucosidase inhibitors from root of C. sieberiana and all the compounds are isolated from this source for the first time. ACG Publications 2017 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/75725/1/HasnahMohdSirat_Bioassay-GuidedIsolationofAntioxidantsanda-Glucosidase.pdf Jibril, S. and Sirat, H. M. and Basar, N. (2017) Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae). Records of Natural Products, 11 (4). pp. 406-410. ISSN 1307-6167 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020407947&partnerID=40&md5=d82667dd644d6e6a0519e8f0108cdbd0
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Jibril, S.
Sirat, H. M.
Basar, N.
Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)
description Bioassay-guided isolation was used to investigate the antioxidants and α-glucosidase inhibitors from extract of root of Cassia sieberiana. The ethyl acetate fraction demonstrated strong free radical scavenging (DPPH and ABTS+) and potent α-glucosidase inhibition. The subsequent fractionation and purification of the ethyl acetate fraction using silica gel chromatography and Sephadex LH-20, yielded; islandicin (1), chrysophanol (2), physcion (3), emodin (4), quercetin (5), kaempferol (6), dihydrokaempferol (7), and piceatannol (8). Quercetin (5) showed the most active antioxidant activity with IC50 values of 1.58 mM and 1.30 mM against DPPH and ABTS+ radicals, followed by piceatannol (8) with IC50 values of 3.96 mM and 3.28 mM, which is better than the standard BHT (with IC50 value 8.93 mM) and trolox (with IC50 value 8.25 mM), for DPPH and ABTS+ radicals scavenging activities, respectively. For the α-glucosidase inhibitory assay, quercetin (5) and piceatannol (8) showed higher potency against α-glucosidase with IC50 values of 5.73 μM and 7.37 μM respectively, than standard quercetin with IC50 value of 9.20 μM and acarbose with IC50 value of 14.12 μM. This study presents the first report on the α-glucosidase inhibitors from root of C. sieberiana and all the compounds are isolated from this source for the first time.
format Article
author Jibril, S.
Sirat, H. M.
Basar, N.
author_facet Jibril, S.
Sirat, H. M.
Basar, N.
author_sort Jibril, S.
title Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)
title_short Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)
title_full Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)
title_fullStr Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)
title_full_unstemmed Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)
title_sort bioassay-guided isolation of antioxidants and ?-glucosidase inhibitors from the root of cassia sieberiana d.c. (fabaceae)
publisher ACG Publications
publishDate 2017
url http://eprints.utm.my/id/eprint/75725/1/HasnahMohdSirat_Bioassay-GuidedIsolationofAntioxidantsanda-Glucosidase.pdf
http://eprints.utm.my/id/eprint/75725/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020407947&partnerID=40&md5=d82667dd644d6e6a0519e8f0108cdbd0
_version_ 1643657143662936064
score 13.211869

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