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Growth of bacillus coagulans using molasses as a nutrient source

Bacillus coagulans (B. coagulans) can be utilized as microbial inoculant to speed up the bioremediation of wastewater. The effectiveness of the microbial inoculant for treatment is highly selective and relying on the characteristics of the wastewater. A feasible carbon source must be first chosen to...

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Main Authors: Sanadi, N. F. A., Van Fan, Y., Leow, C. W., Wong, J. H., Koay, Y. S., Lee, C. T., Chua, L. S., Sarmidi, M. R.
Format: Article
Published: Italian Association of Chemical Engineering - AIDIC 2017
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/75525/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019430838&doi=10.3303%2fCET1756086&partnerID=40&md5=d95825534d1c12f4689a45b76269ca13
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spelling my.utm.755252018-03-29T00:48:22Z http://eprints.utm.my/id/eprint/75525/ Growth of bacillus coagulans using molasses as a nutrient source Sanadi, N. F. A. Van Fan, Y. Leow, C. W. Wong, J. H. Koay, Y. S. Lee, C. T. Chua, L. S. Sarmidi, M. R. TP Chemical technology Bacillus coagulans (B. coagulans) can be utilized as microbial inoculant to speed up the bioremediation of wastewater. The effectiveness of the microbial inoculant for treatment is highly selective and relying on the characteristics of the wastewater. A feasible carbon source must be first chosen to pre-culture the microbe prior to the bioremediation. Commercial nutrient broths are efficient to grow the microbial; they are costly for the treatment of large volume of wastewater treatment. This study aims to evaluate the growth rate of B. coagulans using a cheaper nutrient source, i.e. molasses. The commercial Man, Rogosa and Sharpe (MRS) media was used as a benchmark. The growth rate of B. coagulans was conducted at different concentrations of molasses (1 %, 3 % and 5 % (w/v)) and in the MRS broth as benchmark. B. coagulans grown in the MRS has shown a much higher maximum specific growth rate (0.69 h-1) compared to that grown in 1 % (w/v) molasses (0.14 h-1). No growth was observed in the higher concentrations of molasses (3 and 5 % (w/v)). Measuring the colonyforming units of B. coagulans in both the MRS and molasses mediums validated the results. Molasses is a desirable carbon source as it is relatively cheaper and easily available. More studies are needed to improve the maximum specific growth rate of B. coagulans in 1 % (w/v) of molasses. Italian Association of Chemical Engineering - AIDIC 2017 Article PeerReviewed Sanadi, N. F. A. and Van Fan, Y. and Leow, C. W. and Wong, J. H. and Koay, Y. S. and Lee, C. T. and Chua, L. S. and Sarmidi, M. R. (2017) Growth of bacillus coagulans using molasses as a nutrient source. Chemical Engineering Transactions, 56 . pp. 511-516. ISSN 2283-9216 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019430838&doi=10.3303%2fCET1756086&partnerID=40&md5=d95825534d1c12f4689a45b76269ca13
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Sanadi, N. F. A.
Van Fan, Y.
Leow, C. W.
Wong, J. H.
Koay, Y. S.
Lee, C. T.
Chua, L. S.
Sarmidi, M. R.
Growth of bacillus coagulans using molasses as a nutrient source
description Bacillus coagulans (B. coagulans) can be utilized as microbial inoculant to speed up the bioremediation of wastewater. The effectiveness of the microbial inoculant for treatment is highly selective and relying on the characteristics of the wastewater. A feasible carbon source must be first chosen to pre-culture the microbe prior to the bioremediation. Commercial nutrient broths are efficient to grow the microbial; they are costly for the treatment of large volume of wastewater treatment. This study aims to evaluate the growth rate of B. coagulans using a cheaper nutrient source, i.e. molasses. The commercial Man, Rogosa and Sharpe (MRS) media was used as a benchmark. The growth rate of B. coagulans was conducted at different concentrations of molasses (1 %, 3 % and 5 % (w/v)) and in the MRS broth as benchmark. B. coagulans grown in the MRS has shown a much higher maximum specific growth rate (0.69 h-1) compared to that grown in 1 % (w/v) molasses (0.14 h-1). No growth was observed in the higher concentrations of molasses (3 and 5 % (w/v)). Measuring the colonyforming units of B. coagulans in both the MRS and molasses mediums validated the results. Molasses is a desirable carbon source as it is relatively cheaper and easily available. More studies are needed to improve the maximum specific growth rate of B. coagulans in 1 % (w/v) of molasses.
format Article
author Sanadi, N. F. A.
Van Fan, Y.
Leow, C. W.
Wong, J. H.
Koay, Y. S.
Lee, C. T.
Chua, L. S.
Sarmidi, M. R.
author_facet Sanadi, N. F. A.
Van Fan, Y.
Leow, C. W.
Wong, J. H.
Koay, Y. S.
Lee, C. T.
Chua, L. S.
Sarmidi, M. R.
author_sort Sanadi, N. F. A.
title Growth of bacillus coagulans using molasses as a nutrient source
title_short Growth of bacillus coagulans using molasses as a nutrient source
title_full Growth of bacillus coagulans using molasses as a nutrient source
title_fullStr Growth of bacillus coagulans using molasses as a nutrient source
title_full_unstemmed Growth of bacillus coagulans using molasses as a nutrient source
title_sort growth of bacillus coagulans using molasses as a nutrient source
publisher Italian Association of Chemical Engineering - AIDIC
publishDate 2017
url http://eprints.utm.my/id/eprint/75525/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019430838&doi=10.3303%2fCET1756086&partnerID=40&md5=d95825534d1c12f4689a45b76269ca13
_version_ 1643657087879741440
score 13.211869

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