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Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina

The present study was carried out to optimize the physical and nutritional parameters for cultivation of Trichoderma harzianum Rifai for production of extracellular β-glucosidase, an alternative to chemcial fungicides to control Macrophomina phaseolina, which causes charcoal rot disease in crops. Re...

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Main Authors: Khalili, E., Huyop, F., Myra Abd. Manan, F., Wahab, R. A.
Format: Article
Language:English
Published: Taylor and Francis Ltd. 2017
Subjects:
QH Natural history
Online Access:http://eprints.utm.my/id/eprint/75448/1/ElhamKhalili_OptimizationofCultivationConditionsinBanana.pdf
http://eprints.utm.my/id/eprint/75448/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021101816&doi=10.1080%2f13102818.2017.1342562&partnerID=40&md5=fe4406f9221f25357d74be0ab4befa24
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spelling my.utm.754482018-03-21T00:38:16Z http://eprints.utm.my/id/eprint/75448/ Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina Khalili, E. Huyop, F. Myra Abd. Manan, F. Wahab, R. A. QH Natural history The present study was carried out to optimize the physical and nutritional parameters for cultivation of Trichoderma harzianum Rifai for production of extracellular β-glucosidase, an alternative to chemcial fungicides to control Macrophomina phaseolina, which causes charcoal rot disease in crops. Response surface methodology by the Box–Behnken design (BBD) based on four factors (temperature, carbon sources, inoculum size and pH) was used for optimization of the cultivation conditions to produce β-glucosidase using agro-industrial banana wastes. The highest β-glucosidase activity (1483.27 U/mL) was attained under optimized cultivation conditions: ∼36 ºC and pH 7.3, using carbon source and inoculum size of 10% (w/v) and 5% (w/v), respectively. It is noteworthy that the low deviation values (0.09−0.44%) in the verification experiments (R1, R2 and R3) inferred the generated model was accurate to predict optimal cultivation conditions of T. harzianum Rifai. Likewise, the obtained diameters of inhibition zones ranging from 58.00−38.66 mm following treatment with β-glucosidase was found comparable to other in vitro tests using pure T. harzianum isolates and chemical fungicides. Hence, the findings indicated that it was feasible to use β-glucosidase as a greener alternative to chemical fungicides for control of M. phaseolina infection and consequently, for protection of crops against charcoal rot disease. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Taylor and Francis Ltd. 2017 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/75448/1/ElhamKhalili_OptimizationofCultivationConditionsinBanana.pdf Khalili, E. and Huyop, F. and Myra Abd. Manan, F. and Wahab, R. A. (2017) Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina. Biotechnology and Biotechnological Equipment, 31 (5). pp. 921-934. ISSN 1310-2818 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021101816&doi=10.1080%2f13102818.2017.1342562&partnerID=40&md5=fe4406f9221f25357d74be0ab4befa24
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QH Natural history
spellingShingle QH Natural history
Khalili, E.
Huyop, F.
Myra Abd. Manan, F.
Wahab, R. A.
Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
description The present study was carried out to optimize the physical and nutritional parameters for cultivation of Trichoderma harzianum Rifai for production of extracellular β-glucosidase, an alternative to chemcial fungicides to control Macrophomina phaseolina, which causes charcoal rot disease in crops. Response surface methodology by the Box–Behnken design (BBD) based on four factors (temperature, carbon sources, inoculum size and pH) was used for optimization of the cultivation conditions to produce β-glucosidase using agro-industrial banana wastes. The highest β-glucosidase activity (1483.27 U/mL) was attained under optimized cultivation conditions: ∼36 ºC and pH 7.3, using carbon source and inoculum size of 10% (w/v) and 5% (w/v), respectively. It is noteworthy that the low deviation values (0.09−0.44%) in the verification experiments (R1, R2 and R3) inferred the generated model was accurate to predict optimal cultivation conditions of T. harzianum Rifai. Likewise, the obtained diameters of inhibition zones ranging from 58.00−38.66 mm following treatment with β-glucosidase was found comparable to other in vitro tests using pure T. harzianum isolates and chemical fungicides. Hence, the findings indicated that it was feasible to use β-glucosidase as a greener alternative to chemical fungicides for control of M. phaseolina infection and consequently, for protection of crops against charcoal rot disease. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
format Article
author Khalili, E.
Huyop, F.
Myra Abd. Manan, F.
Wahab, R. A.
author_facet Khalili, E.
Huyop, F.
Myra Abd. Manan, F.
Wahab, R. A.
author_sort Khalili, E.
title Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_short Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_full Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_fullStr Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_full_unstemmed Optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina
title_sort optimization of cultivation conditions in banana wastes for production of extracellular β-glucosidase by trichoderma harzianum rifai efficient for in vitro inhibition of macrophomina phaseolina
publisher Taylor and Francis Ltd.
publishDate 2017
url http://eprints.utm.my/id/eprint/75448/1/ElhamKhalili_OptimizationofCultivationConditionsinBanana.pdf
http://eprints.utm.my/id/eprint/75448/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021101816&doi=10.1080%2f13102818.2017.1342562&partnerID=40&md5=fe4406f9221f25357d74be0ab4befa24
_version_ 1643657067305631744
score 13.211869

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