Scaling-up of simultaneous saccharification and fermentation of lactic acid from microwave-alkali-treated empty fruit bunches
The goal of this work was to scale up the simultaneous saccharification and fermentation (SSF) of lactic acid using microwave-alkali-pretreated empty fruit bunches (EFB) from a scale of 16 L to a scale of 150 L. To facilitate the scaling-up process of lactic acid production by Rhizopus oryzae NRRL 3...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2017
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/75443/1/AniIdris_Scaling-upofSimultaneousSaccharification.pdf http://eprints.utm.my/id/eprint/75443/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032685395&doi=10.15376%2fbiores.12.4.8001-8013&partnerID=40&md5=f25068d3b85841a60b173134caf75e20 |
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| Summary: | The goal of this work was to scale up the simultaneous saccharification and fermentation (SSF) of lactic acid using microwave-alkali-pretreated empty fruit bunches (EFB) from a scale of 16 L to a scale of 150 L. To facilitate the scaling-up process of lactic acid production by Rhizopus oryzae NRRL 395, a scaling-up criterion of constant kLa value was applied. Operating conditions, such as aeration rate and superficial velocity, were varied and evaluated on both scales (16-L and 150-L). The highest lactic acid yield of 6.8 g/L was obtained under an operating condition of 1 vvm (0.061 s-1). Parallel aeration rates were determined for the 150-L fermenter system to obtain the same kLa value as the 16-L fermenter. An operational condition of 0.5 vvm dissolved oxygen supply in the 150-L fermenter was optimal to support an identical value of kLa and production rate of lactic acid for both scales. |
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