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Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications

One of the prerequisites for the functional and structural characterisation of proteins is to obtain a sufficiently high amount of the protein sample. Recombinant protein expression systems are usually employed for the overproduction of proteins in cases where isolation from their native host is dif...

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Main Authors:	Illias, R. M., Ramli, A. N. M., Low, K. O., Muhammad Mahadi, N., Abdul Murad, A. M., Rabu, A.
Format:	Book Section
Published:	Springer-Verlag Berlin Heidelberg 2013
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/74689/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930329366&doi=10.1007%2f978-3-642-39681-6_22&partnerID=40&md5=5dc62ec0fe438c04834587cf4c24180a
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id	my.utm.74689
record_format	eprints
spelling	my.utm.746892017-11-26T04:18:23Z http://eprints.utm.my/id/eprint/74689/ Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications Illias, R. M. Ramli, A. N. M. Low, K. O. Muhammad Mahadi, N. Abdul Murad, A. M. Rabu, A. TP Chemical technology One of the prerequisites for the functional and structural characterisation of proteins is to obtain a sufficiently high amount of the protein sample. Recombinant protein expression systems are usually employed for the overproduction of proteins in cases where isolation from their native host is difficult. For each protein, a suitable expression system must be optimised to obtain the sample in a soluble, correctly folded conformation with high production yield. The recent discovery of psychrophilic yeasts and their potential in industrial applications have sparked interest in overexpression strategies for high-level expression of psychrophilic proteins. Here, we discuss some of the recent developments in the recombinant production of cold-adapted yeast proteins, particularly those from Glaciozyma antarctica PI12, in suitable heterologous hosts. Findings from our work, as well as from recent publications, are included. Springer-Verlag Berlin Heidelberg 2013 Book Section PeerReviewed Illias, R. M. and Ramli, A. N. M. and Low, K. O. and Muhammad Mahadi, N. and Abdul Murad, A. M. and Rabu, A. (2013) Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications. In: Cold-adapted Yeasts: Biodiversity, Adaptation Strategies and Biotechnological Significance. Springer-Verlag Berlin Heidelberg, pp. 481-496. ISBN 978-364239681-6 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930329366&doi=10.1007%2f978-3-642-39681-6_22&partnerID=40&md5=5dc62ec0fe438c04834587cf4c24180a
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Illias, R. M. Ramli, A. N. M. Low, K. O. Muhammad Mahadi, N. Abdul Murad, A. M. Rabu, A. Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications
description	One of the prerequisites for the functional and structural characterisation of proteins is to obtain a sufficiently high amount of the protein sample. Recombinant protein expression systems are usually employed for the overproduction of proteins in cases where isolation from their native host is difficult. For each protein, a suitable expression system must be optimised to obtain the sample in a soluble, correctly folded conformation with high production yield. The recent discovery of psychrophilic yeasts and their potential in industrial applications have sparked interest in overexpression strategies for high-level expression of psychrophilic proteins. Here, we discuss some of the recent developments in the recombinant production of cold-adapted yeast proteins, particularly those from Glaciozyma antarctica PI12, in suitable heterologous hosts. Findings from our work, as well as from recent publications, are included.
format	Book Section
author	Illias, R. M. Ramli, A. N. M. Low, K. O. Muhammad Mahadi, N. Abdul Murad, A. M. Rabu, A.
author_facet	Illias, R. M. Ramli, A. N. M. Low, K. O. Muhammad Mahadi, N. Abdul Murad, A. M. Rabu, A.
author_sort	Illias, R. M.
title	Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications
title_short	Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications
title_full	Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications
title_fullStr	Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications
title_full_unstemmed	Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications
title_sort	heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications
publisher	Springer-Verlag Berlin Heidelberg
publishDate	2013
url	http://eprints.utm.my/id/eprint/74689/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930329366&doi=10.1007%2f978-3-642-39681-6_22&partnerID=40&md5=5dc62ec0fe438c04834587cf4c24180a
_version_	1643656910814052352
score	13.211869

Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications

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