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Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities

The present study is designed to identify various bioactive flavonoid compounds from the methanolic fern extract (MFE) of Cheilanthes tenuifolia. Flavonoids derived from C. tenuifolia (fern) possess potent anti-cancerous, anti-bacterial, anti-oxidant activities that are responsible for their chemo-p...

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Main Authors: Jarial, Rini, Shard, A., Thakur, Sveta, Sakinah, Abdul Munaim, Zularisam, Abd. Wahid, Rezania, Shahabaldin, Kanwar, Shamsher Singh, Singh, Lakhveer
Format: Article
Published: Elsevier B.V. 2016
Subjects:
TA Engineering (General). Civil engineering (General)
Online Access:http://eprints.utm.my/id/eprint/72794/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018191344&doi=10.1016%2fj.jksus.2017.04.007&partnerID=40&md5=69951edd13f6b0ecc8233e0266872a81
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spelling my.utm.727942017-11-20T07:58:23Z http://eprints.utm.my/id/eprint/72794/ Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities Jarial, Rini Shard, A. Thakur, Sveta Sakinah, Abdul Munaim Zularisam, Abd. Wahid Rezania, Shahabaldin Kanwar, Shamsher Singh Singh, Lakhveer TA Engineering (General). Civil engineering (General) The present study is designed to identify various bioactive flavonoid compounds from the methanolic fern extract (MFE) of Cheilanthes tenuifolia. Flavonoids derived from C. tenuifolia (fern) possess potent anti-cancerous, anti-bacterial, anti-oxidant activities that are responsible for their chemo-preventive potential against selected bacterial panel. A preparative column chromatography with a column Sephadex LH-20 was used to isolate and purify flavonoids from C. tenuifolia. Their structure and chemical bonds were identified by using Nuclear Magnetic Resonance (NMR) spectroscopy and Fourier Transform-Infra Red Spectroscopy (FTIR). Two flavonoids were identified as rutin (2.8. mg) and quercetin (3.34. mg) from 100. g of C. tenuifolia. The minimum inhibitory concentration (MIC) values of (2.25 and 0.45. μg/ml) were obtained in purified flavonoids against Staphylococcus aureus and Enterobacter sp., respectively. The two flavonoids (Rutin and quercetin) showed significant in vitro anti-oxidant activity; in this regard, the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging potential of quercetin (86.1%) was higher than that of rutin (73.2%). However, for human hepatoma HepG2 and human carcinoma HeLa cells, quercetin exhibited high anti-cancer activity than rutin. The purified compounds (rutin and quercetin) with having great potential of free radical inactivator in HepG2 cells. The results suggest that MFE of C. tenuifolia could potentially be employed in traditional medicine as they are rich in compounds with anti-oxidant, anti-microbial and anti-cancer properties. Elsevier B.V. 2016 Article PeerReviewed Jarial, Rini and Shard, A. and Thakur, Sveta and Sakinah, Abdul Munaim and Zularisam, Abd. Wahid and Rezania, Shahabaldin and Kanwar, Shamsher Singh and Singh, Lakhveer (2016) Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities. Journal of King Saud University - Science . ISSN 1018-3647 (In Press) https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018191344&doi=10.1016%2fj.jksus.2017.04.007&partnerID=40&md5=69951edd13f6b0ecc8233e0266872a81
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TA Engineering (General). Civil engineering (General)
spellingShingle TA Engineering (General). Civil engineering (General)
Jarial, Rini
Shard, A.
Thakur, Sveta
Sakinah, Abdul Munaim
Zularisam, Abd. Wahid
Rezania, Shahabaldin
Kanwar, Shamsher Singh
Singh, Lakhveer
Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities
description The present study is designed to identify various bioactive flavonoid compounds from the methanolic fern extract (MFE) of Cheilanthes tenuifolia. Flavonoids derived from C. tenuifolia (fern) possess potent anti-cancerous, anti-bacterial, anti-oxidant activities that are responsible for their chemo-preventive potential against selected bacterial panel. A preparative column chromatography with a column Sephadex LH-20 was used to isolate and purify flavonoids from C. tenuifolia. Their structure and chemical bonds were identified by using Nuclear Magnetic Resonance (NMR) spectroscopy and Fourier Transform-Infra Red Spectroscopy (FTIR). Two flavonoids were identified as rutin (2.8. mg) and quercetin (3.34. mg) from 100. g of C. tenuifolia. The minimum inhibitory concentration (MIC) values of (2.25 and 0.45. μg/ml) were obtained in purified flavonoids against Staphylococcus aureus and Enterobacter sp., respectively. The two flavonoids (Rutin and quercetin) showed significant in vitro anti-oxidant activity; in this regard, the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging potential of quercetin (86.1%) was higher than that of rutin (73.2%). However, for human hepatoma HepG2 and human carcinoma HeLa cells, quercetin exhibited high anti-cancer activity than rutin. The purified compounds (rutin and quercetin) with having great potential of free radical inactivator in HepG2 cells. The results suggest that MFE of C. tenuifolia could potentially be employed in traditional medicine as they are rich in compounds with anti-oxidant, anti-microbial and anti-cancer properties.
format Article
author Jarial, Rini
Shard, A.
Thakur, Sveta
Sakinah, Abdul Munaim
Zularisam, Abd. Wahid
Rezania, Shahabaldin
Kanwar, Shamsher Singh
Singh, Lakhveer
author_facet Jarial, Rini
Shard, A.
Thakur, Sveta
Sakinah, Abdul Munaim
Zularisam, Abd. Wahid
Rezania, Shahabaldin
Kanwar, Shamsher Singh
Singh, Lakhveer
author_sort Jarial, Rini
title Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities
title_short Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities
title_full Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities
title_fullStr Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities
title_full_unstemmed Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities
title_sort characterization of flavonoids from fern cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities
publisher Elsevier B.V.
publishDate 2016
url http://eprints.utm.my/id/eprint/72794/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018191344&doi=10.1016%2fj.jksus.2017.04.007&partnerID=40&md5=69951edd13f6b0ecc8233e0266872a81
_version_ 1643656512649822208
score 13.211869

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