A predominant β-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity
Low reaction yields and the high cost of obtaining a single type of pure CD make γ-CD costly. Using rational design and with the aid of 3D modeling structures, recombinant CGTase from Bacillus sp. G1 was molecularly engineered with the aim of producing a higher percentage of γ-CD. A single mutatio...
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| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
Elsevier B.V.
2009
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/7268/ http://dx.doi.org/10.1016/j.molcatb.2008.09.016 |
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