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Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology

The objective of this study was to develop an optimized assay for Salmonella Typhi biofilm that mimics the environment of the gallbladder as an experimental model for chronic typhoid fever. Multi-factorial assays are difficult to optimize using traditional one-factor-at-a-time optimization methods....

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Main Authors: Ganjali Dashti, M., Abdeshahian, P., Sudesh, K., Phua, K. K.
Format: Article
Published: Taylor and Francis Ltd. 2016
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/72623/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961891807&doi=10.1080%2f08927014.2015.1135328&partnerID=40&md5=05d25c3a2f5bb0e884a53d3d0eae05fc
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spelling my.utm.726232017-11-27T04:37:36Z http://eprints.utm.my/id/eprint/72623/ Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology Ganjali Dashti, M. Abdeshahian, P. Sudesh, K. Phua, K. K. TP Chemical technology The objective of this study was to develop an optimized assay for Salmonella Typhi biofilm that mimics the environment of the gallbladder as an experimental model for chronic typhoid fever. Multi-factorial assays are difficult to optimize using traditional one-factor-at-a-time optimization methods. Response surface methodology (RSM) was used to optimize six key variables involved in S. Typhi biofilm formation on cholesterol-coated polypropylene 96-well microtiter plates. The results showed that bile (1.22%), glucose (2%), cholesterol (0.05%) and potassium chloride (0.25%) were critical factors affecting the amount of biofilm produced, but agitation (275 rpm) and sodium chloride (0.5%) had antagonistic effects on each other. Under these optimum conditions the maximum OD reading for biofilm formation was 3.4 (λ600 nm), and the coefficients of variation for intra-plate and inter-plate assays were 3% (n = 20) and 5% (n = 8), respectively. These results showed that RSM is an effective approach for biofilm assay optimization. Taylor and Francis Ltd. 2016 Article PeerReviewed Ganjali Dashti, M. and Abdeshahian, P. and Sudesh, K. and Phua, K. K. (2016) Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology. Biofouling, 32 (4). pp. 477-487. ISSN 0892-7014 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961891807&doi=10.1080%2f08927014.2015.1135328&partnerID=40&md5=05d25c3a2f5bb0e884a53d3d0eae05fc
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Ganjali Dashti, M.
Abdeshahian, P.
Sudesh, K.
Phua, K. K.
Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology
description The objective of this study was to develop an optimized assay for Salmonella Typhi biofilm that mimics the environment of the gallbladder as an experimental model for chronic typhoid fever. Multi-factorial assays are difficult to optimize using traditional one-factor-at-a-time optimization methods. Response surface methodology (RSM) was used to optimize six key variables involved in S. Typhi biofilm formation on cholesterol-coated polypropylene 96-well microtiter plates. The results showed that bile (1.22%), glucose (2%), cholesterol (0.05%) and potassium chloride (0.25%) were critical factors affecting the amount of biofilm produced, but agitation (275 rpm) and sodium chloride (0.5%) had antagonistic effects on each other. Under these optimum conditions the maximum OD reading for biofilm formation was 3.4 (λ600 nm), and the coefficients of variation for intra-plate and inter-plate assays were 3% (n = 20) and 5% (n = 8), respectively. These results showed that RSM is an effective approach for biofilm assay optimization.
format Article
author Ganjali Dashti, M.
Abdeshahian, P.
Sudesh, K.
Phua, K. K.
author_facet Ganjali Dashti, M.
Abdeshahian, P.
Sudesh, K.
Phua, K. K.
author_sort Ganjali Dashti, M.
title Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology
title_short Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology
title_full Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology
title_fullStr Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology
title_full_unstemmed Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology
title_sort optimization of salmonella typhi biofilm assay on polypropylene microtiter plates using response surface methodology
publisher Taylor and Francis Ltd.
publishDate 2016
url http://eprints.utm.my/id/eprint/72623/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961891807&doi=10.1080%2f08927014.2015.1135328&partnerID=40&md5=05d25c3a2f5bb0e884a53d3d0eae05fc
_version_ 1643656481915011072
score 13.211869

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