Staff View: Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231

Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231

Scurrula ferruginea (Jack) Danser is one of the mistletoe species belonging to Loranthaceae family, which grows on the branches of many deciduous trees in tropical countries. This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, w...

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Main Authors:	Marvibaigi, M., Amini, N., Supriyanto, E., Majid, F. A. A., Jaganathan, S. K., Jamil, S., Almaki, J. H., Nasiri, R.
Format:	Article
Published:	Public Library of Science 2016
Subjects:	R Medicine (General)
Online Access:	http://eprints.utm.my/id/eprint/72352/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978656570&doi=10.1371%2fjournal.pone.0158942&partnerID=40&md5=4d2f1171feb9ba5d56eaf7682640fd06
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spelling	my.utm.723522017-11-20T08:23:45Z http://eprints.utm.my/id/eprint/72352/ Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231 Marvibaigi, M. Amini, N. Supriyanto, E. Majid, F. A. A. Jaganathan, S. K. Jamil, S. Almaki, J. H. Nasiri, R. R Medicine (General) Scurrula ferruginea (Jack) Danser is one of the mistletoe species belonging to Loranthaceae family, which grows on the branches of many deciduous trees in tropical countries. This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, which showed potent antioxidant activities, and high phenolic and flavonoid contents, were investigated in human breast cancer cell line (MDA-MB-231) and non-cancer human skin fibroblast cells (HSF-1184). The activities and characteristics varied depending on the different parts of S. ferruginea, solvent polarity, and concentrations of extracts. The stem methanol extract showed the highest amount of both phenolic (273.51 \xB1 4.84 mg gallic acid/g extract) and flavonoid contents (163.41 \xB1 4.62 mg catechin/g extract) and strong DPPH\x95 radical scavenging (IC50 = 27.81 \xB5g/mL) and metal chelation activity (IC50 = 80.20 \xB5g/mL). The stem aqueous extract showed the highest ABTS\x95+ scavenging ability. The stem methanol and aqueous extracts exhibited dose-dependent cytotoxic activity against MDA-MB-231 cells with IC50 of 19.27 and 50.35 \xB5g/mL, respectively. Furthermore, the extracts inhibited the migration and colony formation of MDA-MB-231 cells in a concentration-dependent manner. Morphological observations revealed hallmark properties of apoptosis in treated cells. The methanol extract induced an increase in ROS generation and mitochondrial depolarization in MDA-MB-231 cells, suggesting its potent apoptotic activity. The present study demonstrated that the S. ferruginea methanol extract mediated MDA-MB-231 cell growth inhibition via induction of apoptosis which was confirmed by Western blot analysis. It may be a potential anticancer agent, however, its in vivo anticancer activity needs to be investigated. \xA9 2016 Marvibaigi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Public Library of Science 2016 Article PeerReviewed Marvibaigi, M. and Amini, N. and Supriyanto, E. and Majid, F. A. A. and Jaganathan, S. K. and Jamil, S. and Almaki, J. H. and Nasiri, R. (2016) Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231. PLoS ONE, 11 (7). ISSN 1932-6203 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978656570&doi=10.1371%2fjournal.pone.0158942&partnerID=40&md5=4d2f1171feb9ba5d56eaf7682640fd06
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	R Medicine (General)
spellingShingle	R Medicine (General) Marvibaigi, M. Amini, N. Supriyanto, E. Majid, F. A. A. Jaganathan, S. K. Jamil, S. Almaki, J. H. Nasiri, R. Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231
description	Scurrula ferruginea (Jack) Danser is one of the mistletoe species belonging to Loranthaceae family, which grows on the branches of many deciduous trees in tropical countries. This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, which showed potent antioxidant activities, and high phenolic and flavonoid contents, were investigated in human breast cancer cell line (MDA-MB-231) and non-cancer human skin fibroblast cells (HSF-1184). The activities and characteristics varied depending on the different parts of S. ferruginea, solvent polarity, and concentrations of extracts. The stem methanol extract showed the highest amount of both phenolic (273.51 \xB1 4.84 mg gallic acid/g extract) and flavonoid contents (163.41 \xB1 4.62 mg catechin/g extract) and strong DPPH\x95 radical scavenging (IC50 = 27.81 \xB5g/mL) and metal chelation activity (IC50 = 80.20 \xB5g/mL). The stem aqueous extract showed the highest ABTS\x95+ scavenging ability. The stem methanol and aqueous extracts exhibited dose-dependent cytotoxic activity against MDA-MB-231 cells with IC50 of 19.27 and 50.35 \xB5g/mL, respectively. Furthermore, the extracts inhibited the migration and colony formation of MDA-MB-231 cells in a concentration-dependent manner. Morphological observations revealed hallmark properties of apoptosis in treated cells. The methanol extract induced an increase in ROS generation and mitochondrial depolarization in MDA-MB-231 cells, suggesting its potent apoptotic activity. The present study demonstrated that the S. ferruginea methanol extract mediated MDA-MB-231 cell growth inhibition via induction of apoptosis which was confirmed by Western blot analysis. It may be a potential anticancer agent, however, its in vivo anticancer activity needs to be investigated. \xA9 2016 Marvibaigi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
format	Article
author	Marvibaigi, M. Amini, N. Supriyanto, E. Majid, F. A. A. Jaganathan, S. K. Jamil, S. Almaki, J. H. Nasiri, R.
author_facet	Marvibaigi, M. Amini, N. Supriyanto, E. Majid, F. A. A. Jaganathan, S. K. Jamil, S. Almaki, J. H. Nasiri, R.
author_sort	Marvibaigi, M.
title	Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231
title_short	Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231
title_full	Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231
title_fullStr	Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231
title_full_unstemmed	Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231
title_sort	antioxidant activity and ros-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell mda-mb-231
publisher	Public Library of Science
publishDate	2016
url	http://eprints.utm.my/id/eprint/72352/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978656570&doi=10.1371%2fjournal.pone.0158942&partnerID=40&md5=4d2f1171feb9ba5d56eaf7682640fd06
_version_	1643656417474772992
score	13.211869

Antioxidant activity and ROS-dependent apoptotic effect of scurrula ferruginea (jack) danser methanol extract in human breast cancer cell MDA-MB-231

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