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Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection

Arsenite is an inorganic form of arsenic that poses hazardous effect to human. It is a common environmental heavy metal contaminant ubiquitously found in water and groundwater. In this study, an optical biosensor for arsenite determination was developed by immobilization of crude arsenite oxidase (A...

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Main Authors: Teoh, W. K., Zahari, F., Shahir, S.
Format: Article
Language:English
Published: Penerbit UTM Press 2016
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/72000/1/ShafinazShahir2016_PreliminaryInvestigationsonanEnzymeImmobilized.pdf
http://eprints.utm.my/id/eprint/72000/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84991677419&doi=10.11113%2fjt.v78.7757&partnerID=40&md5=468525a9c5458f7d5019332e7bee7b00
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spelling my.utm.720002017-11-16T06:13:43Z http://eprints.utm.my/id/eprint/72000/ Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection Teoh, W. K. Zahari, F. Shahir, S. Q Science (General) Arsenite is an inorganic form of arsenic that poses hazardous effect to human. It is a common environmental heavy metal contaminant ubiquitously found in water and groundwater. In this study, an optical biosensor for arsenite determination was developed by immobilization of crude arsenite oxidase (Aio) extracted from recombinant E. coli, in chitosan solution coated on triacetyl-cellulose membrane employing DCPIP as colour indicator. The arsenite oxidase (Aio) was successfully expressed and extracted from recombinant E. coli strain BL21 (DE3). The protein concentration and specific activity of the crude arsenite oxidase were determined. Expression of Aio was confirmed by SDS-PAGE. The crude Aio was also successfully immobilized in chitosan and coated on triacetyl cellulose membrane. The response time and dynamic range of the optical biosensor were optimized. The response time of the developed biosensor was 15 minutes. The amount of DCPIP reduced (ΔA) was inversely proportional to the arsenite concentration. Standard calibration curve for arsenite detection was achieved within the range of arsenite concentration from 25 μM to 200 μM. The maximum detection limit was determined to be 250 μM arsenite. Penerbit UTM Press 2016 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/72000/1/ShafinazShahir2016_PreliminaryInvestigationsonanEnzymeImmobilized.pdf Teoh, W. K. and Zahari, F. and Shahir, S. (2016) Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection. Jurnal Teknologi, 78 (10). pp. 17-21. ISSN 0127-9696 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84991677419&doi=10.11113%2fjt.v78.7757&partnerID=40&md5=468525a9c5458f7d5019332e7bee7b00
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Teoh, W. K.
Zahari, F.
Shahir, S.
Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection
description Arsenite is an inorganic form of arsenic that poses hazardous effect to human. It is a common environmental heavy metal contaminant ubiquitously found in water and groundwater. In this study, an optical biosensor for arsenite determination was developed by immobilization of crude arsenite oxidase (Aio) extracted from recombinant E. coli, in chitosan solution coated on triacetyl-cellulose membrane employing DCPIP as colour indicator. The arsenite oxidase (Aio) was successfully expressed and extracted from recombinant E. coli strain BL21 (DE3). The protein concentration and specific activity of the crude arsenite oxidase were determined. Expression of Aio was confirmed by SDS-PAGE. The crude Aio was also successfully immobilized in chitosan and coated on triacetyl cellulose membrane. The response time and dynamic range of the optical biosensor were optimized. The response time of the developed biosensor was 15 minutes. The amount of DCPIP reduced (ΔA) was inversely proportional to the arsenite concentration. Standard calibration curve for arsenite detection was achieved within the range of arsenite concentration from 25 μM to 200 μM. The maximum detection limit was determined to be 250 μM arsenite.
format Article
author Teoh, W. K.
Zahari, F.
Shahir, S.
author_facet Teoh, W. K.
Zahari, F.
Shahir, S.
author_sort Teoh, W. K.
title Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection
title_short Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection
title_full Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection
title_fullStr Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection
title_full_unstemmed Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection
title_sort preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection
publisher Penerbit UTM Press
publishDate 2016
url http://eprints.utm.my/id/eprint/72000/1/ShafinazShahir2016_PreliminaryInvestigationsonanEnzymeImmobilized.pdf
http://eprints.utm.my/id/eprint/72000/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84991677419&doi=10.11113%2fjt.v78.7757&partnerID=40&md5=468525a9c5458f7d5019332e7bee7b00
_version_ 1643656333650558976
score 13.211869

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