Staff View: Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)

Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)

Watercress (Nasturtium officinale sp.) from the Brassicae family contains phenethyl glucosinolates (gluconasturtiin) as the main glucosinolate (GLS). The enzymatic hydrolysis products by naturally-occuring myrosinase produced phenethyl isothicyanate (PEITC) which reported to possess anti-carcinogeni...

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Main Authors:	Mohd. Zul, S., Surugau, N.
Format:	Article
Published:	Penerbit UTM Press 2016
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://eprints.utm.my/id/eprint/71581/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979708959&doi=10.11113%2fjt.v78.5092&partnerID=40&md5=ee3ba3e1ee21154cbab7c22f54ffdb60
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id	my.utm.71581
record_format	eprints
spelling	my.utm.715812017-11-20T08:28:24Z http://eprints.utm.my/id/eprint/71581/ Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.) Mohd. Zul, S. Surugau, N. TA Engineering (General). Civil engineering (General) Watercress (Nasturtium officinale sp.) from the Brassicae family contains phenethyl glucosinolates (gluconasturtiin) as the main glucosinolate (GLS). The enzymatic hydrolysis products by naturally-occuring myrosinase produced phenethyl isothicyanate (PEITC) which reported to possess anti-carcinogenic activity. Depending on several factors, its counterpart, phenethyl nitrile (PEN) can also be formed as hydrolysis product. This study investigated the effects of adding ascorbic acid and Fe2+ ions at different concentration on the hydrolysis of gluconasturtiin. Hydrolysis products were extracted using dichloromethane and analyzed semi-quantitatively by using GCMS. The results showed that PEITC increased at the low concentration of ascorbic acid (up to 0.06M). Similarly, addition of up to 0.06M Fe2+ ions increased PEITC; higher than 0.06M inhibits the formation of PEITC. Interestingly, similar trend for the production of PEN was detected. This study also investigated myrosinase activity both by exogenous and endogenous methods at different concentrations of ascorbic acid and Fe2+ ions using standard sinigrin as subsrat. Overall, the myrosinase activity was more active at the low concentrations of ascorbic acid. Also, the exogenous method is more efficient than endogenous. This study proved that the presence of reducing agents such as ascorbic acid and Fe2+ ions during the preparation of watercress as food would affect the production of the health-promoting PEITC. Penerbit UTM Press 2016 Article PeerReviewed Mohd. Zul, S. and Surugau, N. (2016) Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.). Jurnal Teknologi, 78 (8). pp. 133-138. ISSN 0127-9696 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979708959&doi=10.11113%2fjt.v78.5092&partnerID=40&md5=ee3ba3e1ee21154cbab7c22f54ffdb60
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Mohd. Zul, S. Surugau, N. Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)
description	Watercress (Nasturtium officinale sp.) from the Brassicae family contains phenethyl glucosinolates (gluconasturtiin) as the main glucosinolate (GLS). The enzymatic hydrolysis products by naturally-occuring myrosinase produced phenethyl isothicyanate (PEITC) which reported to possess anti-carcinogenic activity. Depending on several factors, its counterpart, phenethyl nitrile (PEN) can also be formed as hydrolysis product. This study investigated the effects of adding ascorbic acid and Fe2+ ions at different concentration on the hydrolysis of gluconasturtiin. Hydrolysis products were extracted using dichloromethane and analyzed semi-quantitatively by using GCMS. The results showed that PEITC increased at the low concentration of ascorbic acid (up to 0.06M). Similarly, addition of up to 0.06M Fe2+ ions increased PEITC; higher than 0.06M inhibits the formation of PEITC. Interestingly, similar trend for the production of PEN was detected. This study also investigated myrosinase activity both by exogenous and endogenous methods at different concentrations of ascorbic acid and Fe2+ ions using standard sinigrin as subsrat. Overall, the myrosinase activity was more active at the low concentrations of ascorbic acid. Also, the exogenous method is more efficient than endogenous. This study proved that the presence of reducing agents such as ascorbic acid and Fe2+ ions during the preparation of watercress as food would affect the production of the health-promoting PEITC.
format	Article
author	Mohd. Zul, S. Surugau, N.
author_facet	Mohd. Zul, S. Surugau, N.
author_sort	Mohd. Zul, S.
title	Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)
title_short	Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)
title_full	Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)
title_fullStr	Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)
title_full_unstemmed	Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)
title_sort	effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (nasturtium officinale sp.)
publisher	Penerbit UTM Press
publishDate	2016
url	http://eprints.utm.my/id/eprint/71581/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979708959&doi=10.11113%2fjt.v78.5092&partnerID=40&md5=ee3ba3e1ee21154cbab7c22f54ffdb60
_version_	1643656222565466112
score	13.211869

Effects of ascorbic acid and ferum ions concentration on the hydrolysis of glucosinolate and myrosinase activity in the watercress (Nasturtium officinale sp.)

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