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Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis

Micelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in met...

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Main Authors: Thang, Lee Yien, See, Hong Heng, Quirino, Joselito P.
Format: Article
Published: Wiley-VCH Verlag 2016
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/69173/
http://dx.doi.org/10.1002/elps.201600010
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spelling my.utm.691732017-11-20T08:52:17Z http://eprints.utm.my/id/eprint/69173/ Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis Thang, Lee Yien See, Hong Heng Quirino, Joselito P. QD Chemistry Micelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in methanol and the resulting sample solution was pressure injected after a micellar plug of SDS. Factors that affected the stacking such as SDS concentration, micelle, and sample plug length were examined. The sensitivity enhancement factor (peak height from stacking/peak height from typical injection of sample in BGE) was 15-22. The method detection limits with LLE were in the range of 5-10 ng/mL, which was lower than the established method (where the LLE extract was also prepared in methanol) with reported method detection limits of 25-40 ng/mL. The intraday and interday repeatability were in the range of 1.0-3.4% and 3.8-6.5%, respectively. Wiley-VCH Verlag 2016 Article PeerReviewed Thang, Lee Yien and See, Hong Heng and Quirino, Joselito P. (2016) Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis. Electrophoresis, 37 (9). pp. 1166-1169. ISSN 0173-0835 http://dx.doi.org/10.1002/elps.201600010 DOI:10.1002/elps.201600010
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
spellingShingle QD Chemistry
Thang, Lee Yien
See, Hong Heng
Quirino, Joselito P.
Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
description Micelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in methanol and the resulting sample solution was pressure injected after a micellar plug of SDS. Factors that affected the stacking such as SDS concentration, micelle, and sample plug length were examined. The sensitivity enhancement factor (peak height from stacking/peak height from typical injection of sample in BGE) was 15-22. The method detection limits with LLE were in the range of 5-10 ng/mL, which was lower than the established method (where the LLE extract was also prepared in methanol) with reported method detection limits of 25-40 ng/mL. The intraday and interday repeatability were in the range of 1.0-3.4% and 3.8-6.5%, respectively.
format Article
author Thang, Lee Yien
See, Hong Heng
Quirino, Joselito P.
author_facet Thang, Lee Yien
See, Hong Heng
Quirino, Joselito P.
author_sort Thang, Lee Yien
title Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
title_short Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
title_full Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
title_fullStr Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
title_full_unstemmed Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
title_sort determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
publisher Wiley-VCH Verlag
publishDate 2016
url http://eprints.utm.my/id/eprint/69173/
http://dx.doi.org/10.1002/elps.201600010
_version_ 1643656022533865472
score 13.211869

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