Cover Image

Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro

The present study describes a dynamic system that can be used in a three- dimensional (3D) in vitro cell culture environment which promotes new microvessel formation. Human umbilical vein endothelial cells (HUVECs) were used in this study to form the inner lining of the microvessel, guided by the dy...

Full description

Saved in:
Bibliographic Details
Main Authors: Mohd. Rofi, Mohd. Ramdan, Sukmana, Irza, Rashidi, Nur Syazana, Mat Noor, Noor Jasmawati, Abdul Kadir, Mohammed Rafiq, Syahrom, Ardiyansyah
Format: Article
Published: Asian Research Publishing Network (ARPN) 2017
Subjects:
TJ Mechanical engineering and machinery
R Medicine
Online Access:http://eprints.utm.my/id/eprint/66502/
http://www.arpnjournals.org/jeas/research_papers/rp_2017/jeas_0117_5628.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.66502
record_format eprints
spelling my.utm.665022017-10-03T13:34:22Z http://eprints.utm.my/id/eprint/66502/ Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro Mohd. Rofi, Mohd. Ramdan Sukmana, Irza Rashidi, Nur Syazana Mat Noor, Noor Jasmawati Abdul Kadir, Mohammed Rafiq Syahrom, Ardiyansyah TJ Mechanical engineering and machinery R Medicine The present study describes a dynamic system that can be used in a three- dimensional (3D) in vitro cell culture environment which promotes new microvessel formation. Human umbilical vein endothelial cells (HUVECs) were used in this study to form the inner lining of the microvessel, guided by the dynamic flow produced by our system within a 3D matrix made from fibrin. A 2D environment was used as a comparison. The dynamic flow was set to produce 5, 10, 15 or 20 dynes/cm2 shear stress to the cells in culture. Cultured HUVECs were observed for the increase in cell numbers and formation of microvessels. Cells cultured after 2 days demonstrated an increase in cell numbers when subjected to 10dynes/cm2 or more. By day 4, cells appeared to have altered morphologies and were oriented towards the direction of fluid flow. From the fluorescence images observed, it became apparent that there were microvascular channels forming in the 3D cultures. Our dynamic flow system appears to influence endothelial cells to promote microvascular formations in a 3D environment. Asian Research Publishing Network (ARPN) 2017-01-01 Article PeerReviewed Mohd. Rofi, Mohd. Ramdan and Sukmana, Irza and Rashidi, Nur Syazana and Mat Noor, Noor Jasmawati and Abdul Kadir, Mohammed Rafiq and Syahrom, Ardiyansyah (2017) Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro. ARPN Journal of Engineering and Applied Sciences, 12 (2). pp. 371-376. ISSN 1819-6608 http://www.arpnjournals.org/jeas/research_papers/rp_2017/jeas_0117_5628.pdf
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TJ Mechanical engineering and machinery
R Medicine
spellingShingle TJ Mechanical engineering and machinery
R Medicine
Mohd. Rofi, Mohd. Ramdan
Sukmana, Irza
Rashidi, Nur Syazana
Mat Noor, Noor Jasmawati
Abdul Kadir, Mohammed Rafiq
Syahrom, Ardiyansyah
Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro
description The present study describes a dynamic system that can be used in a three- dimensional (3D) in vitro cell culture environment which promotes new microvessel formation. Human umbilical vein endothelial cells (HUVECs) were used in this study to form the inner lining of the microvessel, guided by the dynamic flow produced by our system within a 3D matrix made from fibrin. A 2D environment was used as a comparison. The dynamic flow was set to produce 5, 10, 15 or 20 dynes/cm2 shear stress to the cells in culture. Cultured HUVECs were observed for the increase in cell numbers and formation of microvessels. Cells cultured after 2 days demonstrated an increase in cell numbers when subjected to 10dynes/cm2 or more. By day 4, cells appeared to have altered morphologies and were oriented towards the direction of fluid flow. From the fluorescence images observed, it became apparent that there were microvascular channels forming in the 3D cultures. Our dynamic flow system appears to influence endothelial cells to promote microvascular formations in a 3D environment.
format Article
author Mohd. Rofi, Mohd. Ramdan
Sukmana, Irza
Rashidi, Nur Syazana
Mat Noor, Noor Jasmawati
Abdul Kadir, Mohammed Rafiq
Syahrom, Ardiyansyah
author_facet Mohd. Rofi, Mohd. Ramdan
Sukmana, Irza
Rashidi, Nur Syazana
Mat Noor, Noor Jasmawati
Abdul Kadir, Mohammed Rafiq
Syahrom, Ardiyansyah
author_sort Mohd. Rofi, Mohd. Ramdan
title Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro
title_short Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro
title_full Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro
title_fullStr Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro
title_full_unstemmed Three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro
title_sort three-dimensional advance dynamic culture system promotes microvessel development from cultured endothelial cells in vitro
publisher Asian Research Publishing Network (ARPN)
publishDate 2017
url http://eprints.utm.my/id/eprint/66502/
http://www.arpnjournals.org/jeas/research_papers/rp_2017/jeas_0117_5628.pdf
_version_ 1643655810922840064
score 13.211869

Similar Items