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Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy

A portable microchip electrophoresis (MCE) coupled with on-chip contactless conductivity detection (C4D) system was evaluated for the determination of vancomycin in human plasma. In order to enhance the detection sensitivity, a new online multi-stacking preconcentration technique based on field-enha...

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Main Authors: Chong, Kah Chun, Thang, Lee Yien, Joselito, P. Quirino, See, Hong Heng
Format: Article
Published: Elsevier Science BV 2017
Subjects:
Q Science
Online Access:http://eprints.utm.my/id/eprint/66124/
http://dx.doi.org/10.1016/j.chroma.2017.01.012
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spelling my.utm.661242017-07-11T07:41:17Z http://eprints.utm.my/id/eprint/66124/ Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy Chong, Kah Chun Thang, Lee Yien Joselito, P. Quirino See, Hong Heng Q Science A portable microchip electrophoresis (MCE) coupled with on-chip contactless conductivity detection (C4D) system was evaluated for the determination of vancomycin in human plasma. In order to enhance the detection sensitivity, a new online multi-stacking preconcentration technique based on field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in MCE-C4D system equipped with a commercially available double T-junction glass chip. The cationic analytes from the two sample reservoirs were injected under FESI conditions and subsequently focused by MSS within the sample-loading channel. The proposed multi-stacking strategy was verified under a fluorescence microscope using Rhodamine 6G as the model analyte and a sensitivity enhancement factor (SEF) of up to 217 was achieved. The developed approach was subsequently implemented in the aqueous-based MCE, coupled to C4D in order to monitor the targeted antibiotic (in this case, vancomycin) present in human plasma samples. The multi-stacking and analysis time for vancomycin were 50 s and 250 s respectively, with SEF of approximately 83 when compared to typical gated injection. The detection limit of the method for vancomycin was 1.2 μg/mL, with intraday and interday repeatability RSDs of 2.6% and 4.3%, respectively. Recoveries in spiked human plasma were 99.0%–99.2%. Elsevier Science BV 2017-01-02 Article PeerReviewed Chong, Kah Chun and Thang, Lee Yien and Joselito, P. Quirino and See, Hong Heng (2017) Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy. Journal of Chromatography A, 1485 . pp. 142-146. ISSN 0021-9673 http://dx.doi.org/10.1016/j.chroma.2017.01.012 DOI:10.1016/j.chroma.2017.01.012
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science
spellingShingle Q Science
Chong, Kah Chun
Thang, Lee Yien
Joselito, P. Quirino
See, Hong Heng
Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy
description A portable microchip electrophoresis (MCE) coupled with on-chip contactless conductivity detection (C4D) system was evaluated for the determination of vancomycin in human plasma. In order to enhance the detection sensitivity, a new online multi-stacking preconcentration technique based on field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in MCE-C4D system equipped with a commercially available double T-junction glass chip. The cationic analytes from the two sample reservoirs were injected under FESI conditions and subsequently focused by MSS within the sample-loading channel. The proposed multi-stacking strategy was verified under a fluorescence microscope using Rhodamine 6G as the model analyte and a sensitivity enhancement factor (SEF) of up to 217 was achieved. The developed approach was subsequently implemented in the aqueous-based MCE, coupled to C4D in order to monitor the targeted antibiotic (in this case, vancomycin) present in human plasma samples. The multi-stacking and analysis time for vancomycin were 50 s and 250 s respectively, with SEF of approximately 83 when compared to typical gated injection. The detection limit of the method for vancomycin was 1.2 μg/mL, with intraday and interday repeatability RSDs of 2.6% and 4.3%, respectively. Recoveries in spiked human plasma were 99.0%–99.2%.
format Article
author Chong, Kah Chun
Thang, Lee Yien
Joselito, P. Quirino
See, Hong Heng
author_facet Chong, Kah Chun
Thang, Lee Yien
Joselito, P. Quirino
See, Hong Heng
author_sort Chong, Kah Chun
title Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy
title_short Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy
title_full Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy
title_fullStr Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy
title_full_unstemmed Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy
title_sort monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy
publisher Elsevier Science BV
publishDate 2017
url http://eprints.utm.my/id/eprint/66124/
http://dx.doi.org/10.1016/j.chroma.2017.01.012
_version_ 1643655763859603456
score 13.211869

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