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Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island

Industries and agriculture activities extensively utilize halogenated compounds. These compounds were found to be toxic and pollute the environment. Thus, many studies have been done on microbial degradation of these chemicals. In this study, an attempt was made to isolate bacterial strains EK1-EK5...

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Main Authors: Huyop, Fahrul Zaman, Khosrowabadi, Elham
Format: Article
Published: Taylor & Francis Inc 2014
Subjects:
QH Natural history
Online Access:http://eprints.utm.my/id/eprint/62525/
http://dx.doi.org/10.1080/10889868.2013.834868
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spelling my.utm.625252017-06-18T00:19:17Z http://eprints.utm.my/id/eprint/62525/ Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island Huyop, Fahrul Zaman Khosrowabadi, Elham QH Natural history Industries and agriculture activities extensively utilize halogenated compounds. These compounds were found to be toxic and pollute the environment. Thus, many studies have been done on microbial degradation of these chemicals. In this study, an attempt was made to isolate bacterial strains EK1-EK5 from marine sediments collected at Danga Bay and east coast of Singapore island. The 16S rRNA analysis suggested that the isolated bacteria had more than 96% sequence identity to the sequence in the database; therefore, they were designated as Bacillus sp., Rhodococcus sp., Lysinibacillus sp., Microbacterium sp., and Aminobacter sp. The results of molecular analysis were supported by biochemical and microscopic examinations. Bacterial isolates were able to grow slowly in minimal medium containing only 2,2-dichloropropionate as the sole carbon source. The cellular doubling times were 39.60 ± 0.1, 36.60 ± 0.2, 30.71 ± 0.1, 41.23 ± 0.1, and 36.70 ± 0.3 h for EK1, EK2, EK3, EK4, and EK5, respectively. In the future, it will be important to further investigate the presence of the dehalogenase gene in their genomic DNA for further characterization. Taylor & Francis Inc 2014 Article PeerReviewed Huyop, Fahrul Zaman and Khosrowabadi, Elham (2014) Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island. Bioremediation Journal, 18 (1). pp. 20-27. ISSN 1088-9868 http://dx.doi.org/10.1080/10889868.2013.834868 DOI:10.1080/10889868.2013.834868
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QH Natural history
spellingShingle QH Natural history
Huyop, Fahrul Zaman
Khosrowabadi, Elham
Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island
description Industries and agriculture activities extensively utilize halogenated compounds. These compounds were found to be toxic and pollute the environment. Thus, many studies have been done on microbial degradation of these chemicals. In this study, an attempt was made to isolate bacterial strains EK1-EK5 from marine sediments collected at Danga Bay and east coast of Singapore island. The 16S rRNA analysis suggested that the isolated bacteria had more than 96% sequence identity to the sequence in the database; therefore, they were designated as Bacillus sp., Rhodococcus sp., Lysinibacillus sp., Microbacterium sp., and Aminobacter sp. The results of molecular analysis were supported by biochemical and microscopic examinations. Bacterial isolates were able to grow slowly in minimal medium containing only 2,2-dichloropropionate as the sole carbon source. The cellular doubling times were 39.60 ± 0.1, 36.60 ± 0.2, 30.71 ± 0.1, 41.23 ± 0.1, and 36.70 ± 0.3 h for EK1, EK2, EK3, EK4, and EK5, respectively. In the future, it will be important to further investigate the presence of the dehalogenase gene in their genomic DNA for further characterization.
format Article
author Huyop, Fahrul Zaman
Khosrowabadi, Elham
author_facet Huyop, Fahrul Zaman
Khosrowabadi, Elham
author_sort Huyop, Fahrul Zaman
title Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island
title_short Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island
title_full Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island
title_fullStr Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island
title_full_unstemmed Screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of Singapore Island
title_sort screening and characterization of several 2,2-dicholoropropionic acid-degrading bacteria isolated from marine sediment of danga bay and east coast of singapore island
publisher Taylor & Francis Inc
publishDate 2014
url http://eprints.utm.my/id/eprint/62525/
http://dx.doi.org/10.1080/10889868.2013.834868
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score 13.211869

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