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Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation

Pineapple waste is one of the most abundant agricultural wastes found in tropic region. Continual recycling of the solid agricultural waste promotes sustainable biological systems while reduces the cost of managing the solid waste problem. Utilisation of solid waste for the production of value added...

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Main Authors: Azima, Siti Nurbalqis, Tumari, Hasnaa’ Hazimah, Mohd. Zain, Nor Azimah
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
R Medicine (General)
Online Access:http://eprints.utm.my/id/eprint/61358/1/NorAzimahZain2014_ScreeningofLacticAcidProductionfromSolidPineapple.pdf
http://eprints.utm.my/id/eprint/61358/
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spelling my.utm.613582017-03-31T01:37:01Z http://eprints.utm.my/id/eprint/61358/ Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation Azima, Siti Nurbalqis Tumari, Hasnaa’ Hazimah Mohd. Zain, Nor Azimah R Medicine (General) Pineapple waste is one of the most abundant agricultural wastes found in tropic region. Continual recycling of the solid agricultural waste promotes sustainable biological systems while reduces the cost of managing the solid waste problem. Utilisation of solid waste for the production of value added product has been less reported compared to that of liquid wastes. This study is looking into the potential of utilising solid pineapple waste in solid state fermentation for the production of lactic acid by Rhizopus oryzae. A 2-level factorial design was employed to screen the effect of moisture content (60% and 80%), inoculum size (1×104 spores/mL and 1×108 spores/mL), pH (4.5 and 6.5), temperature (27°C and 40°C) and particle size (<0.5 mm and >3.15 mm) to the production of lactic acid. The maximum production of 0.2211 g/L lactic acid was predicted in SSF condition of 80% moisture at pH 6.5, added with 1×104 spores/mL inoculum, with waste particle of 2.0 mm, incubated at 27°C. Analysis of variance (ANOVA) showed that the model is significant with high value of predicted (0.9581) and adjusted (0.9726) R-squared, indicated to a good agreement between the predicted and actual values at each point of the experiment. Post-statistical experiment confirmed the ability of lactic acid production by R. oryzae at the predicted conditions with 0.2367 g/L lactic acid being produced. Finding from this study shows the potential use of solid pineapple waste for the production of value-added product and opens opportunity for further investigation. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/61358/1/NorAzimahZain2014_ScreeningofLacticAcidProductionfromSolidPineapple.pdf Azima, Siti Nurbalqis and Tumari, Hasnaa’ Hazimah and Mohd. Zain, Nor Azimah (2014) Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation. In: 4th International Biotechnology & Biodiversity Conference & Exhibition, 25-27 August, 2014, Johor Bahru, Malaysia.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Azima, Siti Nurbalqis
Tumari, Hasnaa’ Hazimah
Mohd. Zain, Nor Azimah
Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation
description Pineapple waste is one of the most abundant agricultural wastes found in tropic region. Continual recycling of the solid agricultural waste promotes sustainable biological systems while reduces the cost of managing the solid waste problem. Utilisation of solid waste for the production of value added product has been less reported compared to that of liquid wastes. This study is looking into the potential of utilising solid pineapple waste in solid state fermentation for the production of lactic acid by Rhizopus oryzae. A 2-level factorial design was employed to screen the effect of moisture content (60% and 80%), inoculum size (1×104 spores/mL and 1×108 spores/mL), pH (4.5 and 6.5), temperature (27°C and 40°C) and particle size (<0.5 mm and >3.15 mm) to the production of lactic acid. The maximum production of 0.2211 g/L lactic acid was predicted in SSF condition of 80% moisture at pH 6.5, added with 1×104 spores/mL inoculum, with waste particle of 2.0 mm, incubated at 27°C. Analysis of variance (ANOVA) showed that the model is significant with high value of predicted (0.9581) and adjusted (0.9726) R-squared, indicated to a good agreement between the predicted and actual values at each point of the experiment. Post-statistical experiment confirmed the ability of lactic acid production by R. oryzae at the predicted conditions with 0.2367 g/L lactic acid being produced. Finding from this study shows the potential use of solid pineapple waste for the production of value-added product and opens opportunity for further investigation.
format Conference or Workshop Item
author Azima, Siti Nurbalqis
Tumari, Hasnaa’ Hazimah
Mohd. Zain, Nor Azimah
author_facet Azima, Siti Nurbalqis
Tumari, Hasnaa’ Hazimah
Mohd. Zain, Nor Azimah
author_sort Azima, Siti Nurbalqis
title Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation
title_short Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation
title_full Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation
title_fullStr Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation
title_full_unstemmed Screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation
title_sort screening of lactic acid production from solid pineapple waste using rhizopus oryzae via solid state fermentation
publishDate 2014
url http://eprints.utm.my/id/eprint/61358/1/NorAzimahZain2014_ScreeningofLacticAcidProductionfromSolidPineapple.pdf
http://eprints.utm.my/id/eprint/61358/
_version_ 1643655147273846784
score 13.211869

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