Staff View: Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method

Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method

Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, a...

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Main Authors:	Mohammad, Soltani, Al-Ali, Mohanad, Othman, Nor Zalina, Abd. Malek, Roslinda, Elmarzugi, Nagib Ali, Aziz, Ramlan, Ali El-Enshasy, Hesham Ali Metwally
Format:	Article
Published:	IOSR 2015
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/60324/ http://dx.doi.org/10.9790/3008-10117982
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id	my.utm.60324
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spelling	my.utm.603242021-08-02T05:54:57Z http://eprints.utm.my/id/eprint/60324/ Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method Mohammad, Soltani Al-Ali, Mohanad Othman, Nor Zalina Abd. Malek, Roslinda Elmarzugi, Nagib Ali Aziz, Ramlan Ali El-Enshasy, Hesham Ali Metwally TP Chemical technology Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth diameter was monitored during 21 days of cultivation using two series of experiments of different medium supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA medium supplemented with 6 g/L ME and 4 g/L. YE. IOSR 2015 Article PeerReviewed Mohammad, Soltani and Al-Ali, Mohanad and Othman, Nor Zalina and Abd. Malek, Roslinda and Elmarzugi, Nagib Ali and Aziz, Ramlan and Ali El-Enshasy, Hesham Ali Metwally (2015) Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method. IOSR Journal Of Pharmacy And Biological Sciences, 10 (1). pp. 79-82. ISSN 0278-3008 http://dx.doi.org/10.9790/3008-10117982
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mohammad, Soltani Al-Ali, Mohanad Othman, Nor Zalina Abd. Malek, Roslinda Elmarzugi, Nagib Ali Aziz, Ramlan Ali El-Enshasy, Hesham Ali Metwally Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method
description	Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth diameter was monitored during 21 days of cultivation using two series of experiments of different medium supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA medium supplemented with 6 g/L ME and 4 g/L. YE.
format	Article
author	Mohammad, Soltani Al-Ali, Mohanad Othman, Nor Zalina Abd. Malek, Roslinda Elmarzugi, Nagib Ali Aziz, Ramlan Ali El-Enshasy, Hesham Ali Metwally
author_facet	Mohammad, Soltani Al-Ali, Mohanad Othman, Nor Zalina Abd. Malek, Roslinda Elmarzugi, Nagib Ali Aziz, Ramlan Ali El-Enshasy, Hesham Ali Metwally
author_sort	Mohammad, Soltani
title	Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method
title_short	Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method
title_full	Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method
title_fullStr	Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method
title_full_unstemmed	Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method
title_sort	medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method
publisher	IOSR
publishDate	2015
url	http://eprints.utm.my/id/eprint/60324/ http://dx.doi.org/10.9790/3008-10117982
_version_	1707765862964920320
score	13.211869

Medium compositions effects on growth kinetics of cordyceps militaris cells using agar plate method

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