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Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent

Cyanide is highly toxic to the living organisms as it inhibits respiration system in the cell mitochondria. Cyanide iscommonly used in gold extraction process and its discharge into the environment not only causes pollution but it alsobrings harm to the surrounding population. Chemical treatment is...

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Main Authors: Tiong, Belinda, Mohd. Bahari, Zaratulnur, Lee, Nor Sahslin Irwan Shah, Jaafar, Jafariah, Ibrahim, Zaharah, Shahir, Shafinaz
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2015
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/58198/1/BelindaTiong2015_CyanideDegradationbyPseudomonasPseudoalcaligenes.pdf
http://eprints.utm.my/id/eprint/58198/
http://dx.doi.org/10.17576/jsm-2015-4402-10
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spelling my.utm.581982021-09-07T10:46:30Z http://eprints.utm.my/id/eprint/58198/ Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent Tiong, Belinda Mohd. Bahari, Zaratulnur Lee, Nor Sahslin Irwan Shah Jaafar, Jafariah Ibrahim, Zaharah Shahir, Shafinaz Q Science (General) Cyanide is highly toxic to the living organisms as it inhibits respiration system in the cell mitochondria. Cyanide iscommonly used in gold extraction process and its discharge into the environment not only causes pollution but it alsobrings harm to the surrounding population. Chemical treatment is expensive and the use of hazardous compound canexacerbate the problem. Biodegradation offers cheap and safe alternative as it overcomes the problems faced by chemicaltreatment. In this study, indigenous bacteria from mining wastewater were isolated. Cyanide degradation was done viashake flask method. A bacterium, designated W2 was found able to grow in the mining wastewater. 16S rRNAanalysisidentified the strain asPseudomonas pseudoalcaligeneswhich could tolerate up to 39 mg/L cyanide concentration andgrowth was depleted at 52 mg/L. 60% cyanide degradation was achieved in wastewater containing medium. End-productanalysis from high performance liquid chromatography (HPLC) detected formamide implicating the role of cyanidehydratase in cyanide degradation. It can be concluded thatP. pseudoalcaligenesis capable of biodegrading cyanideand its potential in wastewater treatment containing cyanide is feasible. Penerbit Universiti Kebangsaan Malaysia 2015-02-01 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/58198/1/BelindaTiong2015_CyanideDegradationbyPseudomonasPseudoalcaligenes.pdf Tiong, Belinda and Mohd. Bahari, Zaratulnur and Lee, Nor Sahslin Irwan Shah and Jaafar, Jafariah and Ibrahim, Zaharah and Shahir, Shafinaz (2015) Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent. Sains Malaysia, 44 (2). pp. 233-238. ISSN 1266-039 http://dx.doi.org/10.17576/jsm-2015-4402-10 DOI:10.17576/jsm-2015-4402-10
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Tiong, Belinda
Mohd. Bahari, Zaratulnur
Lee, Nor Sahslin Irwan Shah
Jaafar, Jafariah
Ibrahim, Zaharah
Shahir, Shafinaz
Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent
description Cyanide is highly toxic to the living organisms as it inhibits respiration system in the cell mitochondria. Cyanide iscommonly used in gold extraction process and its discharge into the environment not only causes pollution but it alsobrings harm to the surrounding population. Chemical treatment is expensive and the use of hazardous compound canexacerbate the problem. Biodegradation offers cheap and safe alternative as it overcomes the problems faced by chemicaltreatment. In this study, indigenous bacteria from mining wastewater were isolated. Cyanide degradation was done viashake flask method. A bacterium, designated W2 was found able to grow in the mining wastewater. 16S rRNAanalysisidentified the strain asPseudomonas pseudoalcaligeneswhich could tolerate up to 39 mg/L cyanide concentration andgrowth was depleted at 52 mg/L. 60% cyanide degradation was achieved in wastewater containing medium. End-productanalysis from high performance liquid chromatography (HPLC) detected formamide implicating the role of cyanidehydratase in cyanide degradation. It can be concluded thatP. pseudoalcaligenesis capable of biodegrading cyanideand its potential in wastewater treatment containing cyanide is feasible.
format Article
author Tiong, Belinda
Mohd. Bahari, Zaratulnur
Lee, Nor Sahslin Irwan Shah
Jaafar, Jafariah
Ibrahim, Zaharah
Shahir, Shafinaz
author_facet Tiong, Belinda
Mohd. Bahari, Zaratulnur
Lee, Nor Sahslin Irwan Shah
Jaafar, Jafariah
Ibrahim, Zaharah
Shahir, Shafinaz
author_sort Tiong, Belinda
title Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent
title_short Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent
title_full Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent
title_fullStr Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent
title_full_unstemmed Cyanide degradation by Pseudomonas pseudoalcaligenes strain W2 isolated from mining effluent
title_sort cyanide degradation by pseudomonas pseudoalcaligenes strain w2 isolated from mining effluent
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2015
url http://eprints.utm.my/id/eprint/58198/1/BelindaTiong2015_CyanideDegradationbyPseudomonasPseudoalcaligenes.pdf
http://eprints.utm.my/id/eprint/58198/
http://dx.doi.org/10.17576/jsm-2015-4402-10
_version_ 1712285031838253056
score 13.211869

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