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Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101

Denitrification plays a significant role in both wastewater treatment and nitrogen cycle. But high amount of nitrate and accumulation of nitrite inhibit these processes. In view of this, a denitrifying phototrophic bacterium was isolated, characterized based on 16s rRNA analysis and its ability to r...

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Main Authors: Idi, Ahmad, Ibrahim, Zaharah, Mohamad, Shaza Eva, Abdul Majid, Zaiton
Format: Article
Published: Elsevier Ltd. 2015
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/57958/
http://dx.doi.org/10.1016/j.ibiod.2015.09.014
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spelling my.utm.579582022-04-10T03:04:35Z http://eprints.utm.my/id/eprint/57958/ Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101 Idi, Ahmad Ibrahim, Zaharah Mohamad, Shaza Eva Abdul Majid, Zaiton QD Chemistry Denitrification plays a significant role in both wastewater treatment and nitrogen cycle. But high amount of nitrate and accumulation of nitrite inhibit these processes. In view of this, a denitrifying phototrophic bacterium was isolated, characterized based on 16s rRNA analysis and its ability to remove toxic nitrogenous compounds investigated. Different initial concentrations were used to determine the effect of concentration on nitrogen removal and the biokinetic coefficients using Michaelis-Menten rate expression. The bacterium can remove 71% of nitrate at the initial concentration of 85 mg l-1 under photoheterotrophic growth condition without nitrite or ammonium accumulation. It can also remove 62% of ammonium at initial concentrations of 52 mg l-1 under anoxic growth condition without nitrate or nitrite accumulation. The results also showed that increase in concentrations led to decrease for both NO3- and NH4-N removal. Analysis and amplification of the possible genes that are involved in denitrification revealed the possession of napA and nirK genes. This strain can therefore serve as good candidate in wastewater treatment plants that are faced with the problem of nitrite accumulation and high amount of nitrogenous compounds that inhibit cell growth during biological nitrogen removal. Elsevier Ltd. 2015 Article PeerReviewed Idi, Ahmad and Ibrahim, Zaharah and Mohamad, Shaza Eva and Abdul Majid, Zaiton (2015) Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101. International Biodeterioration and Biodegradation, 105 . pp. 245-251. ISSN 0964-8305 http://dx.doi.org/10.1016/j.ibiod.2015.09.014 DOI:10.1016/j.ibiod.2015.09.014
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
spellingShingle QD Chemistry
Idi, Ahmad
Ibrahim, Zaharah
Mohamad, Shaza Eva
Abdul Majid, Zaiton
Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101
description Denitrification plays a significant role in both wastewater treatment and nitrogen cycle. But high amount of nitrate and accumulation of nitrite inhibit these processes. In view of this, a denitrifying phototrophic bacterium was isolated, characterized based on 16s rRNA analysis and its ability to remove toxic nitrogenous compounds investigated. Different initial concentrations were used to determine the effect of concentration on nitrogen removal and the biokinetic coefficients using Michaelis-Menten rate expression. The bacterium can remove 71% of nitrate at the initial concentration of 85 mg l-1 under photoheterotrophic growth condition without nitrite or ammonium accumulation. It can also remove 62% of ammonium at initial concentrations of 52 mg l-1 under anoxic growth condition without nitrate or nitrite accumulation. The results also showed that increase in concentrations led to decrease for both NO3- and NH4-N removal. Analysis and amplification of the possible genes that are involved in denitrification revealed the possession of napA and nirK genes. This strain can therefore serve as good candidate in wastewater treatment plants that are faced with the problem of nitrite accumulation and high amount of nitrogenous compounds that inhibit cell growth during biological nitrogen removal.
format Article
author Idi, Ahmad
Ibrahim, Zaharah
Mohamad, Shaza Eva
Abdul Majid, Zaiton
author_facet Idi, Ahmad
Ibrahim, Zaharah
Mohamad, Shaza Eva
Abdul Majid, Zaiton
author_sort Idi, Ahmad
title Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101
title_short Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101
title_full Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101
title_fullStr Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101
title_full_unstemmed Biokinetics of nitrogen removal at high concentrations by Rhodobacter sphaeroides ADZ101
title_sort biokinetics of nitrogen removal at high concentrations by rhodobacter sphaeroides adz101
publisher Elsevier Ltd.
publishDate 2015
url http://eprints.utm.my/id/eprint/57958/
http://dx.doi.org/10.1016/j.ibiod.2015.09.014
_version_ 1729703222357000192
score 13.211869

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