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An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

The current demands of sustainable green methodologies have increased the use of enzymatic technology in industrial processes. Employment of enzyme as biocatalysts offers the benefits of mild reaction conditions, biodegradability and catalytic efficiency. The harsh conditions of industrial processes...

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Main Authors: Mohamad, Nur Royhaila, Che Marzuki, Nur Haziqah, Buang, Nor Aziah, Huyop, Fahrul, Abdul Wahab, Roswanira
Format: Article
Published: Diagnosis Press Limited 2015
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/57790/
http://www.dx.doi.org/10.1080/13102818.2015.1008192
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spelling my.utm.577902021-09-30T06:01:17Z http://eprints.utm.my/id/eprint/57790/ An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes Mohamad, Nur Royhaila Che Marzuki, Nur Haziqah Buang, Nor Aziah Huyop, Fahrul Abdul Wahab, Roswanira QD Chemistry The current demands of sustainable green methodologies have increased the use of enzymatic technology in industrial processes. Employment of enzyme as biocatalysts offers the benefits of mild reaction conditions, biodegradability and catalytic efficiency. The harsh conditions of industrial processes, however, increase propensity of enzyme destabilization, shortening their industrial lifespan. Consequently, the technology of enzyme immobilization provides an effective means to circumvent these concerns by enhancing enzyme catalytic properties and also simplify downstream processing and improve operational stability. There are several techniques used to immobilize the enzymes onto supports which range from reversible physical adsorption and ionic linkages, to the irreversible stable covalent bonds. Such techniques produce immobilized enzymes of varying stability due to changes in the surface microenvironment and degree of multipoint attachment. Hence, it is mandatory to obtain information about the structure of the enzyme protein following interaction with the support surface as well as interactions of the enzymes with other proteins. Characterization technologies at the nanoscale level to study enzymes immobilized on surfaces are crucial to obtain valuable qualitative and quantitative information, including morphological visualization of the immobilized enzymes. These technologies are pertinent to assess efficacy of an immobilization technique and development of future enzyme immobilization strategies. Diagnosis Press Limited 2015 Article PeerReviewed Mohamad, Nur Royhaila and Che Marzuki, Nur Haziqah and Buang, Nor Aziah and Huyop, Fahrul and Abdul Wahab, Roswanira (2015) An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes. Biotechnology And Biotechnological Equipment, 29 (2). pp. 205-220. ISSN 1310-2818 http://www.dx.doi.org/10.1080/13102818.2015.1008192 DOI: 10.1080/13102818.2015.1008192
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
spellingShingle QD Chemistry
Mohamad, Nur Royhaila
Che Marzuki, Nur Haziqah
Buang, Nor Aziah
Huyop, Fahrul
Abdul Wahab, Roswanira
An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
description The current demands of sustainable green methodologies have increased the use of enzymatic technology in industrial processes. Employment of enzyme as biocatalysts offers the benefits of mild reaction conditions, biodegradability and catalytic efficiency. The harsh conditions of industrial processes, however, increase propensity of enzyme destabilization, shortening their industrial lifespan. Consequently, the technology of enzyme immobilization provides an effective means to circumvent these concerns by enhancing enzyme catalytic properties and also simplify downstream processing and improve operational stability. There are several techniques used to immobilize the enzymes onto supports which range from reversible physical adsorption and ionic linkages, to the irreversible stable covalent bonds. Such techniques produce immobilized enzymes of varying stability due to changes in the surface microenvironment and degree of multipoint attachment. Hence, it is mandatory to obtain information about the structure of the enzyme protein following interaction with the support surface as well as interactions of the enzymes with other proteins. Characterization technologies at the nanoscale level to study enzymes immobilized on surfaces are crucial to obtain valuable qualitative and quantitative information, including morphological visualization of the immobilized enzymes. These technologies are pertinent to assess efficacy of an immobilization technique and development of future enzyme immobilization strategies.
format Article
author Mohamad, Nur Royhaila
Che Marzuki, Nur Haziqah
Buang, Nor Aziah
Huyop, Fahrul
Abdul Wahab, Roswanira
author_facet Mohamad, Nur Royhaila
Che Marzuki, Nur Haziqah
Buang, Nor Aziah
Huyop, Fahrul
Abdul Wahab, Roswanira
author_sort Mohamad, Nur Royhaila
title An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
title_short An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
title_full An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
title_fullStr An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
title_full_unstemmed An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
title_sort overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
publisher Diagnosis Press Limited
publishDate 2015
url http://eprints.utm.my/id/eprint/57790/
http://www.dx.doi.org/10.1080/13102818.2015.1008192
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score 13.211869

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