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Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion

Nowadays there is an intensely usage of natural bioactive materials as medicinal agent in pharmaceutical industries. Swietenia macrophylla (SM) has been reported to have biological activities such as anti-inflammatory, anti-mutagencity, anti-fungal and anti-tumor activities. Therefore, SM oil was us...

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Main Author: Masoud Eid, Ahmad Mustafa
Format: Thesis
Language:English
Published: 2015
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/54705/1/AhmadMustafaMasoudEidPFChE2015.pdf
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spelling my.utm.547052020-11-03T08:52:35Z http://eprints.utm.my/id/eprint/54705/ Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion Masoud Eid, Ahmad Mustafa TP Chemical technology Nowadays there is an intensely usage of natural bioactive materials as medicinal agent in pharmaceutical industries. Swietenia macrophylla (SM) has been reported to have biological activities such as anti-inflammatory, anti-mutagencity, anti-fungal and anti-tumor activities. Therefore, SM oil was used in the formulation of oral nanoemulsion as self-nanoemulsifying system and topical nanoemulsion as nanoemulgel. In this study, self-nanoemulsifying formulations were prepared for both SM and olive oils using different surfactants/co-surfactants nonionic surfactants (Labrasol, Tween 20, Capmul and Labrafil). For topical preparation, nanoemulgels of SM and olive oils were prepared, first by mixing the oil, glycerol with sucrose ester (Laureate, Oleate and Palmitate) to produce pre-nanoemulsion by heat inversion technique. Then nanoemulsion was formed upon the mixing with water by selfemulsification technique. After that Carbopol (934 and 940) was added to nanoemulsion to produce the nanoemulgel. Finally safety and pharmacology studies were conducted for the prepared formulations. The acute and sub-acute toxicity studies were conducted for SM oil self-nanoemulsifying system on rats. The biochemical parameters were evaluated by studying serum alanine transferase (ALT), aspartate transferase (AST) and alkaline phosphatase (ALP). The histopathology of the rats liver sections were studied under light microscope after staining with haematoxylin and eosin. On the other hand, the anti-inflammatory property for SM oil was screened using carrageenan induced rat paw edema method for SM oil for both oral (self-nanoemulsifying system) and topical (nanoemulgel) preparations. The optimal self-nanoemulsifying formulations were prepared using Tween20/Labrafil (2:1) and Capmul/Labrasol (1:2), producing small droplets size of <150 nm, low polydispersity index below 0.3 and relatively good stability with zeta potential lower than -30. It was found that 50% oil with sucrose laureate (20%) and glycerol (30%) was able to produce a very stable nanoemulsion when stored at 4 °C with droplets size less than 150 nm, with low size distribution below 0.2 and low zeta potential -40. The optimal nanoemulsion formulation was mixed with water and different grades of Carbopol (934 and 940) to produce nanoemulgel. It was found that 0.5% Carbopol 940 produces a stable nanoemulgel and it showed high stability when stored at different temperatures 4 °C, 25 °C and 40 °C for one year with no significant influence on the oil droplets size, size distribution and zeta potential. In addition, the anti-inflammatory properties of Swietenia macrophylla oil were greater in the form of self-nanoemulsifying system and nanoemulgel than in the oil solution. Swietenia macrophylla oil in the form of self-nanoemulsifying system possesses no toxicity effect on rats after acute and sub-acute studies. In conclusion, Swietenia macrophylla oil showed a safety profile with improvement in the anti-inflammatory activity with the help of nanoemulsion. 2015-05 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/54705/1/AhmadMustafaMasoudEidPFChE2015.pdf Masoud Eid, Ahmad Mustafa (2015) Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion. PhD thesis, Universiti Teknologi Malaysia, Faculty of Chemical Engineering. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:94648
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Masoud Eid, Ahmad Mustafa
Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
description Nowadays there is an intensely usage of natural bioactive materials as medicinal agent in pharmaceutical industries. Swietenia macrophylla (SM) has been reported to have biological activities such as anti-inflammatory, anti-mutagencity, anti-fungal and anti-tumor activities. Therefore, SM oil was used in the formulation of oral nanoemulsion as self-nanoemulsifying system and topical nanoemulsion as nanoemulgel. In this study, self-nanoemulsifying formulations were prepared for both SM and olive oils using different surfactants/co-surfactants nonionic surfactants (Labrasol, Tween 20, Capmul and Labrafil). For topical preparation, nanoemulgels of SM and olive oils were prepared, first by mixing the oil, glycerol with sucrose ester (Laureate, Oleate and Palmitate) to produce pre-nanoemulsion by heat inversion technique. Then nanoemulsion was formed upon the mixing with water by selfemulsification technique. After that Carbopol (934 and 940) was added to nanoemulsion to produce the nanoemulgel. Finally safety and pharmacology studies were conducted for the prepared formulations. The acute and sub-acute toxicity studies were conducted for SM oil self-nanoemulsifying system on rats. The biochemical parameters were evaluated by studying serum alanine transferase (ALT), aspartate transferase (AST) and alkaline phosphatase (ALP). The histopathology of the rats liver sections were studied under light microscope after staining with haematoxylin and eosin. On the other hand, the anti-inflammatory property for SM oil was screened using carrageenan induced rat paw edema method for SM oil for both oral (self-nanoemulsifying system) and topical (nanoemulgel) preparations. The optimal self-nanoemulsifying formulations were prepared using Tween20/Labrafil (2:1) and Capmul/Labrasol (1:2), producing small droplets size of <150 nm, low polydispersity index below 0.3 and relatively good stability with zeta potential lower than -30. It was found that 50% oil with sucrose laureate (20%) and glycerol (30%) was able to produce a very stable nanoemulsion when stored at 4 °C with droplets size less than 150 nm, with low size distribution below 0.2 and low zeta potential -40. The optimal nanoemulsion formulation was mixed with water and different grades of Carbopol (934 and 940) to produce nanoemulgel. It was found that 0.5% Carbopol 940 produces a stable nanoemulgel and it showed high stability when stored at different temperatures 4 °C, 25 °C and 40 °C for one year with no significant influence on the oil droplets size, size distribution and zeta potential. In addition, the anti-inflammatory properties of Swietenia macrophylla oil were greater in the form of self-nanoemulsifying system and nanoemulgel than in the oil solution. Swietenia macrophylla oil in the form of self-nanoemulsifying system possesses no toxicity effect on rats after acute and sub-acute studies. In conclusion, Swietenia macrophylla oil showed a safety profile with improvement in the anti-inflammatory activity with the help of nanoemulsion.
format Thesis
author Masoud Eid, Ahmad Mustafa
author_facet Masoud Eid, Ahmad Mustafa
author_sort Masoud Eid, Ahmad Mustafa
title Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_short Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_full Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_fullStr Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_full_unstemmed Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_sort improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
publishDate 2015
url http://eprints.utm.my/id/eprint/54705/1/AhmadMustafaMasoudEidPFChE2015.pdf
http://eprints.utm.my/id/eprint/54705/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:94648
_version_ 1683230731162091520
score 13.211869

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