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Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17

Our ultimate dependence on fossil fuels will lead to dramatic depletion of these limited resources. Some developed countries have started to use renewable energy, which efficiently utilizes physical or environmental resources. Hydrogen has been expected to be the energy carrier of the future to repl...

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Main Author: Lagehr, Naglea A. E.
Format: Thesis
Language:English
Published: 2015
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/54590/25/NagleaAELagehrMFBME2015.pdf
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spelling my.utm.545902020-10-20T02:19:34Z http://eprints.utm.my/id/eprint/54590/ Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17 Lagehr, Naglea A. E. Q Science (General) Our ultimate dependence on fossil fuels will lead to dramatic depletion of these limited resources. Some developed countries have started to use renewable energy, which efficiently utilizes physical or environmental resources. Hydrogen has been expected to be the energy carrier of the future to replace fossil fuels. Hydrogen can currently be produced using many methods, most of which use fossil fuels. Biological hydrogen production is gaining wider interest now due to the cleaner production process. Hydrogenase is among the main enzymes involved in bacterial hydrogen production. Among the four types of hydrogenases reported, hydrogenase 3 is considered as the enzyme responsible for hydrogen production only. The others are involved in hydrogen production and utilization. In this research, Citrobacter sp. strain L17 was used. 15 genes in the putative hydrogenase 3 operon of this particular bacterial strain have been successfully detected and amplified. All the clustered genes have been predicted to be coded by 13,456 nucleotides. It has been predicted to be translated into 15 different proteins of 4,485 amino acids. EXPASY TRANSLATE was used to predict the amino acid sequence of the proteins coded by the hydrogenase 3 operon. The results of this study will guide future research of biohydrogen generation by Citrobacter sp. L17, in terms of maximizing biohydrogen yield via metabolic engineering. 2015-01 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/54590/25/NagleaAELagehrMFBME2015.pdf Lagehr, Naglea A. E. (2015) Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17. Masters thesis, Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:86033
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Lagehr, Naglea A. E.
Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17
description Our ultimate dependence on fossil fuels will lead to dramatic depletion of these limited resources. Some developed countries have started to use renewable energy, which efficiently utilizes physical or environmental resources. Hydrogen has been expected to be the energy carrier of the future to replace fossil fuels. Hydrogen can currently be produced using many methods, most of which use fossil fuels. Biological hydrogen production is gaining wider interest now due to the cleaner production process. Hydrogenase is among the main enzymes involved in bacterial hydrogen production. Among the four types of hydrogenases reported, hydrogenase 3 is considered as the enzyme responsible for hydrogen production only. The others are involved in hydrogen production and utilization. In this research, Citrobacter sp. strain L17 was used. 15 genes in the putative hydrogenase 3 operon of this particular bacterial strain have been successfully detected and amplified. All the clustered genes have been predicted to be coded by 13,456 nucleotides. It has been predicted to be translated into 15 different proteins of 4,485 amino acids. EXPASY TRANSLATE was used to predict the amino acid sequence of the proteins coded by the hydrogenase 3 operon. The results of this study will guide future research of biohydrogen generation by Citrobacter sp. L17, in terms of maximizing biohydrogen yield via metabolic engineering.
format Thesis
author Lagehr, Naglea A. E.
author_facet Lagehr, Naglea A. E.
author_sort Lagehr, Naglea A. E.
title Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17
title_short Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17
title_full Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17
title_fullStr Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17
title_full_unstemmed Detection and amplification of putative hydrogenase 3 operon in Citrobacter sp. L17
title_sort detection and amplification of putative hydrogenase 3 operon in citrobacter sp. l17
publishDate 2015
url http://eprints.utm.my/id/eprint/54590/25/NagleaAELagehrMFBME2015.pdf
http://eprints.utm.my/id/eprint/54590/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:86033
_version_ 1681489464050843648
score 13.211869

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