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Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy

Recently, in pharmaceutical research and industry scientists widely have used polysaccharides and other cationic polymer which is one of the most extensive studies in the field of non-viral DNA carriers for gene delivery and therapy. As a purpose of present study variations of the final solution pH...

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Main Authors:	Alireza, Lari, Sultana, Naznin, Zulkifli, Razauden Mohamed Ohamed
Format:	Article
Published:	2014
Subjects:	QH Natural history
Online Access:	http://eprints.utm.my/id/eprint/54581/ http://dx.doi.org/10.4028/www.scientific.net/AMR.1030-1032.2364
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id	my.utm.54581
record_format	eprints
spelling	my.utm.545812017-09-12T08:50:38Z http://eprints.utm.my/id/eprint/54581/ Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy Alireza, Lari Sultana, Naznin Zulkifli, Razauden Mohamed Ohamed QH Natural history Recently, in pharmaceutical research and industry scientists widely have used polysaccharides and other cationic polymer which is one of the most extensive studies in the field of non-viral DNA carriers for gene delivery and therapy. As a purpose of present study variations of the final solution pH values and filtration were examined for their effects on the particle size and the tendency of particle formation. Chitosan nanoparticles were prepared based on the ionic gelatin of chitosan which hydroxyapatite will adsorb onto the chitosan nanoparticles to form complexes of chitosan and hydroxyapatite. The resulting nanoparticles had a size and positive electrical charge, which vary depending on the formulation conditions. The physicochemical properties of the nanoparticles were determined by scanning electron microscope (SEM). Besides of that, element and chemical characterization of samples were assessed by Energy-dispersive X-ray spectroscopy (EDX). The data revealed that the chitosan/DNA nanoparticles were successfully prepared with a nano size range. Obtained complexes could be loaded by variants of DNA for further use in gene delivery applications. 2014 Article PeerReviewed Alireza, Lari and Sultana, Naznin and Zulkifli, Razauden Mohamed Ohamed (2014) Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy. Advanced Materials Research, 1030-1 . pp. 2364-2367. ISSN 1022-6680 http://dx.doi.org/10.4028/www.scientific.net/AMR.1030-1032.2364
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	QH Natural history
spellingShingle	QH Natural history Alireza, Lari Sultana, Naznin Zulkifli, Razauden Mohamed Ohamed Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy
description	Recently, in pharmaceutical research and industry scientists widely have used polysaccharides and other cationic polymer which is one of the most extensive studies in the field of non-viral DNA carriers for gene delivery and therapy. As a purpose of present study variations of the final solution pH values and filtration were examined for their effects on the particle size and the tendency of particle formation. Chitosan nanoparticles were prepared based on the ionic gelatin of chitosan which hydroxyapatite will adsorb onto the chitosan nanoparticles to form complexes of chitosan and hydroxyapatite. The resulting nanoparticles had a size and positive electrical charge, which vary depending on the formulation conditions. The physicochemical properties of the nanoparticles were determined by scanning electron microscope (SEM). Besides of that, element and chemical characterization of samples were assessed by Energy-dispersive X-ray spectroscopy (EDX). The data revealed that the chitosan/DNA nanoparticles were successfully prepared with a nano size range. Obtained complexes could be loaded by variants of DNA for further use in gene delivery applications.
format	Article
author	Alireza, Lari Sultana, Naznin Zulkifli, Razauden Mohamed Ohamed
author_facet	Alireza, Lari Sultana, Naznin Zulkifli, Razauden Mohamed Ohamed
author_sort	Alireza, Lari
title	Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy
title_short	Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy
title_full	Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy
title_fullStr	Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy
title_full_unstemmed	Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy
title_sort	preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy
publishDate	2014
url	http://eprints.utm.my/id/eprint/54581/ http://dx.doi.org/10.4028/www.scientific.net/AMR.1030-1032.2364
_version_	1643653544792817664
score	13.211869

Preparation and characterization of chitosan-hydroxyapatite nanoparticles for gene therapy

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