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Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL

Microbial aggregation and surface hydrophobicity are two important variables often used to evaluate the initial stage of granules development. Most studies only focused on the development of granules but have not studied the ability of microbial aggregation and surface hydrophobicity (SHb) of bacter...

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Main Author: Alias, Muhammad Anwar
Format: Thesis
Language:English
Published: 2015
Subjects:
TA Engineering (General). Civil engineering (General)
Online Access:http://eprints.utm.my/id/eprint/53640/1/MuhammadAnwarAliasMFKA2015.pdf
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spelling my.utm.536402020-08-23T09:03:43Z http://eprints.utm.my/id/eprint/53640/ Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL Alias, Muhammad Anwar TA Engineering (General). Civil engineering (General) Microbial aggregation and surface hydrophobicity are two important variables often used to evaluate the initial stage of granules development. Most studies only focused on the development of granules but have not studied the ability of microbial aggregation and surface hydrophobicity (SHb) of bacteria in the initial stage of biogranulation process. This study investigated the effect of metal cations in improving granules development based on microbial aggregation and surface hydrophobicity (SHb). Autoaggregation (AAg) and SHb of Brevibacillus panacihumi strain ZB1, Lysinibacillus fusiformis strain ZB2 and Enterococcus faecalis strain ZL cells were studied using batch culture. Synthetic wastewater under aerobic condition with the addition of Ca2+, Mg2+, Al3+, Mn2+ and Zn2+ was applied. Initial screening for AAg and SHb using 2-level factorial design showed that Ca2+ caused a significant increase in these two parameters for all the bacteria. Based on the AAg ratio measured from changes in absorbance of the culture medium, all of the three bacteria were classified as medium AAg. L. fusiformis strain ZB2 had the highest value of AAg by having a compact and large microscopic clustering of cells, followed by B. panacihumi strain ZB1 and E. faecalis strain ZL. The AAg ability of each bacterium was well correlated with the SHb. Addition of selected mixed cations (Ca2+, Mg2+, Al3+and Mn2+) increased the AAg ability of the bacterial strains from 35% to 41% for E. faecalis strain ZL, 43% to 56% for B. panacihumi strain ZB1, and 49% to 57%, for L. fusiformis strain ZB2. The SHb of the investigated bacteria had also increased from 32% to 37% for E. faecalis strain ZL, 45% to 55% for B. panacihumi strain ZB1, and 51% to 57%, for L. fusiformis strain ZB2. Addition of mixed cations has also caused a significant increase in the microbial aggregation and SHb of the mixed bacterial culture. The mixed culture consisting of all bacteria had the highest microbial aggregation (44.7%). On the contrary, the mixed culture consisting of B. panacihumi strain ZB1 and E. faecalis strain ZL had the highest SHb (28.8%). As a conclusion, addition of different cations resulted in an increase of AAg and SHb in individual and consortium of the tested bacteria. 2015-06 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/53640/1/MuhammadAnwarAliasMFKA2015.pdf Alias, Muhammad Anwar (2015) Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL. Masters thesis, Universiti Teknologi Malaysia, Faculty of Civil Engineering. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:85654
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TA Engineering (General). Civil engineering (General)
spellingShingle TA Engineering (General). Civil engineering (General)
Alias, Muhammad Anwar
Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL
description Microbial aggregation and surface hydrophobicity are two important variables often used to evaluate the initial stage of granules development. Most studies only focused on the development of granules but have not studied the ability of microbial aggregation and surface hydrophobicity (SHb) of bacteria in the initial stage of biogranulation process. This study investigated the effect of metal cations in improving granules development based on microbial aggregation and surface hydrophobicity (SHb). Autoaggregation (AAg) and SHb of Brevibacillus panacihumi strain ZB1, Lysinibacillus fusiformis strain ZB2 and Enterococcus faecalis strain ZL cells were studied using batch culture. Synthetic wastewater under aerobic condition with the addition of Ca2+, Mg2+, Al3+, Mn2+ and Zn2+ was applied. Initial screening for AAg and SHb using 2-level factorial design showed that Ca2+ caused a significant increase in these two parameters for all the bacteria. Based on the AAg ratio measured from changes in absorbance of the culture medium, all of the three bacteria were classified as medium AAg. L. fusiformis strain ZB2 had the highest value of AAg by having a compact and large microscopic clustering of cells, followed by B. panacihumi strain ZB1 and E. faecalis strain ZL. The AAg ability of each bacterium was well correlated with the SHb. Addition of selected mixed cations (Ca2+, Mg2+, Al3+and Mn2+) increased the AAg ability of the bacterial strains from 35% to 41% for E. faecalis strain ZL, 43% to 56% for B. panacihumi strain ZB1, and 49% to 57%, for L. fusiformis strain ZB2. The SHb of the investigated bacteria had also increased from 32% to 37% for E. faecalis strain ZL, 45% to 55% for B. panacihumi strain ZB1, and 51% to 57%, for L. fusiformis strain ZB2. Addition of mixed cations has also caused a significant increase in the microbial aggregation and SHb of the mixed bacterial culture. The mixed culture consisting of all bacteria had the highest microbial aggregation (44.7%). On the contrary, the mixed culture consisting of B. panacihumi strain ZB1 and E. faecalis strain ZL had the highest SHb (28.8%). As a conclusion, addition of different cations resulted in an increase of AAg and SHb in individual and consortium of the tested bacteria.
format Thesis
author Alias, Muhammad Anwar
author_facet Alias, Muhammad Anwar
author_sort Alias, Muhammad Anwar
title Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL
title_short Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL
title_full Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL
title_fullStr Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL
title_full_unstemmed Effect of cations on microbial aggregation using brevibacillus panacihumi strain ZB1, lysinibacillus fusiformis strain ZB2 and enterococcus faecalis strain ZL
title_sort effect of cations on microbial aggregation using brevibacillus panacihumi strain zb1, lysinibacillus fusiformis strain zb2 and enterococcus faecalis strain zl
publishDate 2015
url http://eprints.utm.my/id/eprint/53640/1/MuhammadAnwarAliasMFKA2015.pdf
http://eprints.utm.my/id/eprint/53640/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:85654
_version_ 1677781081009422336
score 13.211869

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