Staff View: Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis

Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis

Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the mil...

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Main Authors:	Yap, Theresa Wan Chen, Rabu, Amir, Abu Bakar, Farah Diba, Abdul Rahim, Raha, Md. Illias, Rosli, Mahadi, Nor Muhammad, Abdul Murad, Abdul Munir
Format:	Article
Language:	English
Published:	ScientificWorld Ltd. 2014
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/53007/1/AmirRabu2014_GrowthPhase-DependentProteomesoftheMalaysianIsolated.pdf http://eprints.utm.my/id/eprint/53007/ http://dx.doi.org/10.1155/2014/642891
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spelling	my.utm.530072018-07-19T07:23:26Z http://eprints.utm.my/id/eprint/53007/ Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Md. Illias, Rosli Mahadi, Nor Muhammad Abdul Murad, Abdul Munir TP Chemical technology Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the milk of locally bred cows, was studied to elucidate the physiological changes occurring between the growth phases of this bacterium. In this study, ultraperformance liquid chromatography nanoflow electrospray ionization tandem mass spectrometry (UPLC- nano-ESI-MSE) approach was used for qualitative proteomic analysis. A total of 100 and 121 proteins were identified from the midexponential and early stationary growth phases, respectively, of the L. lactis strain M4. During the exponential phase, the most important reaction was the generation of sufficient energy, whereas, in the early stationary phase, the metabolic energy pathways decreased and the biosynthesis of proteins became more important. Thus, the metabolism of the cells shifted from energy production in the exponential phase to the synthesis of macromolecules in the stationary phase. The resultant proteomes are essential in providing an improved view of the cellular machinery of L. lactis during the transition of growth phases and hence provide insight into various biotechnological applications. ScientificWorld Ltd. 2014 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/53007/1/AmirRabu2014_GrowthPhase-DependentProteomesoftheMalaysianIsolated.pdf Yap, Theresa Wan Chen and Rabu, Amir and Abu Bakar, Farah Diba and Abdul Rahim, Raha and Md. Illias, Rosli and Mahadi, Nor Muhammad and Abdul Murad, Abdul Munir (2014) Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis. Scientific World Journal . ISSN 1537-744X http://dx.doi.org/10.1155/2014/642891 DOI: 10.1155/2014/642891
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Md. Illias, Rosli Mahadi, Nor Muhammad Abdul Murad, Abdul Munir Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis
description	Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the milk of locally bred cows, was studied to elucidate the physiological changes occurring between the growth phases of this bacterium. In this study, ultraperformance liquid chromatography nanoflow electrospray ionization tandem mass spectrometry (UPLC- nano-ESI-MSE) approach was used for qualitative proteomic analysis. A total of 100 and 121 proteins were identified from the midexponential and early stationary growth phases, respectively, of the L. lactis strain M4. During the exponential phase, the most important reaction was the generation of sufficient energy, whereas, in the early stationary phase, the metabolic energy pathways decreased and the biosynthesis of proteins became more important. Thus, the metabolism of the cells shifted from energy production in the exponential phase to the synthesis of macromolecules in the stationary phase. The resultant proteomes are essential in providing an improved view of the cellular machinery of L. lactis during the transition of growth phases and hence provide insight into various biotechnological applications.
format	Article
author	Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Md. Illias, Rosli Mahadi, Nor Muhammad Abdul Murad, Abdul Munir
author_facet	Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Md. Illias, Rosli Mahadi, Nor Muhammad Abdul Murad, Abdul Munir
author_sort	Yap, Theresa Wan Chen
title	Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis
title_short	Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis
title_full	Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis
title_fullStr	Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis
title_full_unstemmed	Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis
title_sort	growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain m4 using label-free qualitative shotgun proteomics analysis
publisher	ScientificWorld Ltd.
publishDate	2014
url	http://eprints.utm.my/id/eprint/53007/1/AmirRabu2014_GrowthPhase-DependentProteomesoftheMalaysianIsolated.pdf http://eprints.utm.my/id/eprint/53007/ http://dx.doi.org/10.1155/2014/642891
_version_	1643653292930105344
score	13.211869

Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis

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