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Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)

Bromelain is a complex of proteolytic enzymes obtained from pineapple (Ananas comosus) fruits and stem. These proteolytic enzymes are tools that promise an impressive number of medical and therapeutic uses, particularly as anti-inflammatory agents, dietary supplements and analgesics. The purpose of...

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Main Authors: Loh, Melissa Pei Shian, Syamsul Baharil, Nur Aishah, Ting, Lee Yu, M., Taher, Abdul Majid, Fadzilah Adibah
Format: Conference or Workshop Item
Language:English
Published: 2005
Subjects:
T Technology (General)
Online Access:http://eprints.utm.my/id/eprint/5270/1/MelissaLohPeiShian2005_PilotScaleExtractionOfProteolyticEnzyme.pdf
http://eprints.utm.my/id/eprint/5270/
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spelling my.utm.52702017-08-30T04:30:56Z http://eprints.utm.my/id/eprint/5270/ Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) Loh, Melissa Pei Shian Syamsul Baharil, Nur Aishah Ting, Lee Yu M., Taher Abdul Majid, Fadzilah Adibah T Technology (General) Bromelain is a complex of proteolytic enzymes obtained from pineapple (Ananas comosus) fruits and stem. These proteolytic enzymes are tools that promise an impressive number of medical and therapeutic uses, particularly as anti-inflammatory agents, dietary supplements and analgesics. The purpose of this study was to develop a pilot scale bromelain extraction process. The bromelain extraction method using pineapple fruit core in this study has yielded a total protein content of 9.33 mg/ml with the proteolytic activity of 1400 GDU/ml. An increase in homogenization cycle to increase enzyme yield has proven to be successful. Triple-loop homogenization has shown three times higher proteolytic activity than non-homogenized sample. 2005 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/5270/1/MelissaLohPeiShian2005_PilotScaleExtractionOfProteolyticEnzyme.pdf Loh, Melissa Pei Shian and Syamsul Baharil, Nur Aishah and Ting, Lee Yu and M., Taher and Abdul Majid, Fadzilah Adibah (2005) Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus). In: 2nd International Conference on Chemical and Bioprocess Engineering in conjunction with 19th Symposium of Malaysian Chemical Engineers (SOMChE 2005), 8-10 December 2005, Kota Kinabalu, Sabah.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Loh, Melissa Pei Shian
Syamsul Baharil, Nur Aishah
Ting, Lee Yu
M., Taher
Abdul Majid, Fadzilah Adibah
Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
description Bromelain is a complex of proteolytic enzymes obtained from pineapple (Ananas comosus) fruits and stem. These proteolytic enzymes are tools that promise an impressive number of medical and therapeutic uses, particularly as anti-inflammatory agents, dietary supplements and analgesics. The purpose of this study was to develop a pilot scale bromelain extraction process. The bromelain extraction method using pineapple fruit core in this study has yielded a total protein content of 9.33 mg/ml with the proteolytic activity of 1400 GDU/ml. An increase in homogenization cycle to increase enzyme yield has proven to be successful. Triple-loop homogenization has shown three times higher proteolytic activity than non-homogenized sample.
format Conference or Workshop Item
author Loh, Melissa Pei Shian
Syamsul Baharil, Nur Aishah
Ting, Lee Yu
M., Taher
Abdul Majid, Fadzilah Adibah
author_facet Loh, Melissa Pei Shian
Syamsul Baharil, Nur Aishah
Ting, Lee Yu
M., Taher
Abdul Majid, Fadzilah Adibah
author_sort Loh, Melissa Pei Shian
title Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
title_short Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
title_full Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
title_fullStr Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
title_full_unstemmed Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
title_sort pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
publishDate 2005
url http://eprints.utm.my/id/eprint/5270/1/MelissaLohPeiShian2005_PilotScaleExtractionOfProteolyticEnzyme.pdf
http://eprints.utm.my/id/eprint/5270/
_version_ 1643644276572160000
score 13.211869

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