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Phenyl boronate column fractionation of high molecular weight RNA

The interactions of borate ion with 1,2-cis-diol groups are useful in fractionating mixtures of biological molecules. Separation of RNAs, up to the size of tRNAs, from other biomolecules using phenyl boronate agarose (PBA) column has been shown. In this work, we used phenyl-boronate agarose column t...

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Main Author: Abdul Latif, Fazilah
Format: Article
Language:English
Published: Faculty of Chemical & Natural Resources Engineering 1989
Subjects:
T Technology (General)
Online Access:http://eprints.utm.my/id/eprint/5214/1/FazilahAbdulLatif1989_PhenylBoronateColumnFractionation.pdf
http://eprints.utm.my/id/eprint/5214/
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spelling my.utm.52142010-06-01T15:27:37Z http://eprints.utm.my/id/eprint/5214/ Phenyl boronate column fractionation of high molecular weight RNA Abdul Latif, Fazilah T Technology (General) The interactions of borate ion with 1,2-cis-diol groups are useful in fractionating mixtures of biological molecules. Separation of RNAs, up to the size of tRNAs, from other biomolecules using phenyl boronate agarose (PBA) column has been shown. In this work, we used phenyl-boronate agarose column to separate normal E. coli 16S ribosomal RNA from 16S ribosomal RNA with a blocked 3'-terminus. The blocking group was a 3',5'-cytidine (5'-32p)-bisphosphate. Using a 30 um boronate/ml of agarose (PBA-30) column we obtained reproducible separations at 22C. The results show that this method is useful in preparing high molecular weight RNA of high specific activity for sequence analysis. It is also effective for separation of nuclease digestion products. Faculty of Chemical & Natural Resources Engineering 1989 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/5214/1/FazilahAbdulLatif1989_PhenylBoronateColumnFractionation.pdf Abdul Latif, Fazilah (1989) Phenyl boronate column fractionation of high molecular weight RNA. Buletin FKKKSA, 3 (2). pp. 11-19. ISSN 0128-0740
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Abdul Latif, Fazilah
Phenyl boronate column fractionation of high molecular weight RNA
description The interactions of borate ion with 1,2-cis-diol groups are useful in fractionating mixtures of biological molecules. Separation of RNAs, up to the size of tRNAs, from other biomolecules using phenyl boronate agarose (PBA) column has been shown. In this work, we used phenyl-boronate agarose column to separate normal E. coli 16S ribosomal RNA from 16S ribosomal RNA with a blocked 3'-terminus. The blocking group was a 3',5'-cytidine (5'-32p)-bisphosphate. Using a 30 um boronate/ml of agarose (PBA-30) column we obtained reproducible separations at 22C. The results show that this method is useful in preparing high molecular weight RNA of high specific activity for sequence analysis. It is also effective for separation of nuclease digestion products.
format Article
author Abdul Latif, Fazilah
author_facet Abdul Latif, Fazilah
author_sort Abdul Latif, Fazilah
title Phenyl boronate column fractionation of high molecular weight RNA
title_short Phenyl boronate column fractionation of high molecular weight RNA
title_full Phenyl boronate column fractionation of high molecular weight RNA
title_fullStr Phenyl boronate column fractionation of high molecular weight RNA
title_full_unstemmed Phenyl boronate column fractionation of high molecular weight RNA
title_sort phenyl boronate column fractionation of high molecular weight rna
publisher Faculty of Chemical & Natural Resources Engineering
publishDate 1989
url http://eprints.utm.my/id/eprint/5214/1/FazilahAbdulLatif1989_PhenylBoronateColumnFractionation.pdf
http://eprints.utm.my/id/eprint/5214/
_version_ 1643644263069646848
score 13.239859

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