Staff View: Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6

Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6

Sphingobium sp. strain SYK-6 is capable of degrading various lignin-derived biaryls. We determined the catabolic pathway of a phenylcoumaran-type compound, dehydrodiconiferyl alcohol (DCA) in SYK-6, and identified some of the DCA catabolism genes. In SYK-6 cells, the alcohol group of DCA was oxidize...

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Main Authors:	Takahashi, Kenji, Kamimura, Naofumi, Hishiyama, Shojiro, Hara, Hirofumi, Kasai, Daisuke, Katayama, Yoshihiro, Fukuda, Masao, Kajita, Shinya, Masai, Eiji
Format:	Article
Published:	Kluwer Academic Publishers 2014
Subjects:	T Technology
Online Access:	http://eprints.utm.my/id/eprint/52104/ http://dx.doi.org/10.1007/s10532-014-9695-0
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spelling	my.utm.521042018-11-30T07:03:52Z http://eprints.utm.my/id/eprint/52104/ Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6 Takahashi, Kenji Kamimura, Naofumi Hishiyama, Shojiro Hara, Hirofumi Kasai, Daisuke Katayama, Yoshihiro Fukuda, Masao Kajita, Shinya Masai, Eiji T Technology Sphingobium sp. strain SYK-6 is capable of degrading various lignin-derived biaryls. We determined the catabolic pathway of a phenylcoumaran-type compound, dehydrodiconiferyl alcohol (DCA) in SYK-6, and identified some of the DCA catabolism genes. In SYK-6 cells, the alcohol group of DCA was oxidized to the carboxyl group, first at the B-ring side chain and then at the A-ring side chain. The resultant metabolite was degraded to 5-formylferulate and vanillin through the decarboxylation and the Cα-Cβ cleavage of the A-ring side chain. Based on the DCA catabolic pathway, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) genes are thought to be involved in the conversion of DCA into an aldehyde intermediate (DCA-L) and the conversion of DCA-L into a carboxylic acid intermediate (DCA-C), respectively. SLG_05620 and SLG_24930, which belong to quinohemoprotein ADH and aryl ADH, respectively, were isolated as the genes responsible for the oxidation of DCA. In addition to these genes, multiple genes similar to SLG_05620 and SLG_24930 were found to confer DCA oxidation activities on Escherichia coli cells. In order to identify the DCA-L dehydrogenase genes, the DCA-L oxidation activities of the SYK-6 gene products of putative twenty-one ALDH genes were examined. Significant activities were observed in the four ALDH gene products, including the SLG_27910 product, which showed the highest activity. The disruption of SLG_27910 caused a decreased conversion of DCA-L, suggesting that SLG_27910 plays an important role in the DCA-L oxidation. In conclusion, no specific gene seems to be solely responsible for the conversion of DCA and DCA-L, however, the multiple genes encoding quinohemoprotein ADH and aryl ADH genes, and four ALDH genes are probably involved in the conversion processes. Kluwer Academic Publishers 2014 Article PeerReviewed Takahashi, Kenji and Kamimura, Naofumi and Hishiyama, Shojiro and Hara, Hirofumi and Kasai, Daisuke and Katayama, Yoshihiro and Fukuda, Masao and Kajita, Shinya and Masai, Eiji (2014) Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6. Biodegradation, 25 (5). pp. 735-745. ISSN 0923-9820 http://dx.doi.org/10.1007/s10532-014-9695-0 DOI: 10.1007/s10532-014-9695-0
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	T Technology
spellingShingle	T Technology Takahashi, Kenji Kamimura, Naofumi Hishiyama, Shojiro Hara, Hirofumi Kasai, Daisuke Katayama, Yoshihiro Fukuda, Masao Kajita, Shinya Masai, Eiji Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6
description	Sphingobium sp. strain SYK-6 is capable of degrading various lignin-derived biaryls. We determined the catabolic pathway of a phenylcoumaran-type compound, dehydrodiconiferyl alcohol (DCA) in SYK-6, and identified some of the DCA catabolism genes. In SYK-6 cells, the alcohol group of DCA was oxidized to the carboxyl group, first at the B-ring side chain and then at the A-ring side chain. The resultant metabolite was degraded to 5-formylferulate and vanillin through the decarboxylation and the Cα-Cβ cleavage of the A-ring side chain. Based on the DCA catabolic pathway, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) genes are thought to be involved in the conversion of DCA into an aldehyde intermediate (DCA-L) and the conversion of DCA-L into a carboxylic acid intermediate (DCA-C), respectively. SLG_05620 and SLG_24930, which belong to quinohemoprotein ADH and aryl ADH, respectively, were isolated as the genes responsible for the oxidation of DCA. In addition to these genes, multiple genes similar to SLG_05620 and SLG_24930 were found to confer DCA oxidation activities on Escherichia coli cells. In order to identify the DCA-L dehydrogenase genes, the DCA-L oxidation activities of the SYK-6 gene products of putative twenty-one ALDH genes were examined. Significant activities were observed in the four ALDH gene products, including the SLG_27910 product, which showed the highest activity. The disruption of SLG_27910 caused a decreased conversion of DCA-L, suggesting that SLG_27910 plays an important role in the DCA-L oxidation. In conclusion, no specific gene seems to be solely responsible for the conversion of DCA and DCA-L, however, the multiple genes encoding quinohemoprotein ADH and aryl ADH genes, and four ALDH genes are probably involved in the conversion processes.
format	Article
author	Takahashi, Kenji Kamimura, Naofumi Hishiyama, Shojiro Hara, Hirofumi Kasai, Daisuke Katayama, Yoshihiro Fukuda, Masao Kajita, Shinya Masai, Eiji
author_facet	Takahashi, Kenji Kamimura, Naofumi Hishiyama, Shojiro Hara, Hirofumi Kasai, Daisuke Katayama, Yoshihiro Fukuda, Masao Kajita, Shinya Masai, Eiji
author_sort	Takahashi, Kenji
title	Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6
title_short	Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6
title_full	Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6
title_fullStr	Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6
title_full_unstemmed	Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6
title_sort	characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in sphingobium sp strain syk-6
publisher	Kluwer Academic Publishers
publishDate	2014
url	http://eprints.utm.my/id/eprint/52104/ http://dx.doi.org/10.1007/s10532-014-9695-0
_version_	1643653153339473920
score	13.211869

Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp strain SYK-6

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