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Application of different feeding strategies in fed batch culture for pullulanase production using sago starch

The production of pullulanase by Bacillus flavothermus KWF-1 in batch and fed batch culture were compared using 2 L bioreactor. In batch culture, 0.0803 U/mL of pullulanase activity with specific activity of 0.0213 U/mg was produced by controlling the agitation speed and temperature at 200 rpm and 5...

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Main Authors:	Shankar, Ravi, Md. Salleh, Madihah, Mohamad, Shaza Eva, K. O., Nur Aswati, Abd. Aziz, Suraini, K., Kamarulzaman
Format:	Article
Published:	Elsevier Ltd. 2014
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/51892/ http://dx.doi.org/10.1016/j.carbpol.2013.10.031
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id	my.utm.51892
record_format	eprints
spelling	my.utm.518922018-11-09T08:29:42Z http://eprints.utm.my/id/eprint/51892/ Application of different feeding strategies in fed batch culture for pullulanase production using sago starch Shankar, Ravi Md. Salleh, Madihah Mohamad, Shaza Eva K. O., Nur Aswati Abd. Aziz, Suraini K., Kamarulzaman Q Science (General) The production of pullulanase by Bacillus flavothermus KWF-1 in batch and fed batch culture were compared using 2 L bioreactor. In batch culture, 0.0803 U/mL of pullulanase activity with specific activity of 0.0213 U/mg was produced by controlling the agitation speed and temperature at 200 rpm and 50 C, respectively. Fed batch production was studied by feeding the culture with different sago starch concentrations in various feeding modes for enhanced pullulanase production. Exponential feeding mode at dilution rate of 0.01/h was the preeminent strategy for enhanced pullulanase production of 0.1710 U/mL with specific activity of 0.066 U/mg. It had shown an increment of pullulanase production and specific activity by 2.1 and 3.1-fold, respectively when compared to batch culture. Increment of pullulanase activity in exponential feeding mode improved hydrolyzation of sago starch into maltotriose and panose by 4.5 and 2.5-fold respectively compared to batch system. Elsevier Ltd. 2014 Article PeerReviewed Shankar, Ravi and Md. Salleh, Madihah and Mohamad, Shaza Eva and K. O., Nur Aswati and Abd. Aziz, Suraini and K., Kamarulzaman (2014) Application of different feeding strategies in fed batch culture for pullulanase production using sago starch. Carbohydrate Polymers, 102 (1). pp. 962-969. ISSN 0144-8617 http://dx.doi.org/10.1016/j.carbpol.2013.10.031
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	Q Science (General)
spellingShingle	Q Science (General) Shankar, Ravi Md. Salleh, Madihah Mohamad, Shaza Eva K. O., Nur Aswati Abd. Aziz, Suraini K., Kamarulzaman Application of different feeding strategies in fed batch culture for pullulanase production using sago starch
description	The production of pullulanase by Bacillus flavothermus KWF-1 in batch and fed batch culture were compared using 2 L bioreactor. In batch culture, 0.0803 U/mL of pullulanase activity with specific activity of 0.0213 U/mg was produced by controlling the agitation speed and temperature at 200 rpm and 50 C, respectively. Fed batch production was studied by feeding the culture with different sago starch concentrations in various feeding modes for enhanced pullulanase production. Exponential feeding mode at dilution rate of 0.01/h was the preeminent strategy for enhanced pullulanase production of 0.1710 U/mL with specific activity of 0.066 U/mg. It had shown an increment of pullulanase production and specific activity by 2.1 and 3.1-fold, respectively when compared to batch culture. Increment of pullulanase activity in exponential feeding mode improved hydrolyzation of sago starch into maltotriose and panose by 4.5 and 2.5-fold respectively compared to batch system.
format	Article
author	Shankar, Ravi Md. Salleh, Madihah Mohamad, Shaza Eva K. O., Nur Aswati Abd. Aziz, Suraini K., Kamarulzaman
author_facet	Shankar, Ravi Md. Salleh, Madihah Mohamad, Shaza Eva K. O., Nur Aswati Abd. Aziz, Suraini K., Kamarulzaman
author_sort	Shankar, Ravi
title	Application of different feeding strategies in fed batch culture for pullulanase production using sago starch
title_short	Application of different feeding strategies in fed batch culture for pullulanase production using sago starch
title_full	Application of different feeding strategies in fed batch culture for pullulanase production using sago starch
title_fullStr	Application of different feeding strategies in fed batch culture for pullulanase production using sago starch
title_full_unstemmed	Application of different feeding strategies in fed batch culture for pullulanase production using sago starch
title_sort	application of different feeding strategies in fed batch culture for pullulanase production using sago starch
publisher	Elsevier Ltd.
publishDate	2014
url	http://eprints.utm.my/id/eprint/51892/ http://dx.doi.org/10.1016/j.carbpol.2013.10.031
_version_	1643653092552474624
score	13.211869

Application of different feeding strategies in fed batch culture for pullulanase production using sago starch

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