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Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells

Coumarin is one of the important compounds of cinnamon which possess many biological activities such as anticoagulation, antithrombotic, antimicrobial and anti-oxidant activities. This study was performed to demonstrate the presence of coumarin in cinnamon stem bark through chromatographic fractiona...

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Main Authors:	Ahmad, Rabiatul Adawiyah, Sayid Abdullah, Sharifah H. A., Serati-Nouri, Hamed, Abd. Majid, Fadzilah Adibah, Sarmidi, Mohamad Roji, Abdul Aziz, Ramlan
Format:	Article
Published:	Colegio de Farmaceuticos de la Provincia de Buenos Aires 2014
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/51879/ http://www.latamjpharm.org/
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id	my.utm.51879
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spelling	my.utm.518792018-11-09T08:29:35Z http://eprints.utm.my/id/eprint/51879/ Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells Ahmad, Rabiatul Adawiyah Sayid Abdullah, Sharifah H. A. Serati-Nouri, Hamed Abd. Majid, Fadzilah Adibah Sarmidi, Mohamad Roji Abdul Aziz, Ramlan TP Chemical technology Coumarin is one of the important compounds of cinnamon which possess many biological activities such as anticoagulation, antithrombotic, antimicrobial and anti-oxidant activities. This study was performed to demonstrate the presence of coumarin in cinnamon stem bark through chromatographic fractionation and exploring the potential anticancer properties of cinnamon extract on human ovarian epithelial carcinoma cell line A2780. Water and solvent extraction, chromatographic fractionation and isolation were done for dried cinnamon. The structure of the isolated compound 1 was elucidated by spectroscopic methods. Separation and quantification of compound 1 in cinnamon water extracts were carried out by HPLC. Identification of the compound 1 was achieved by comparing retention times and UV spectra with the standard curve. Cytotoxicity analysis was performed using MTT assay in which A2780 cells were exposed to different concentrations of cinnamon water extract and compound 1. Compound 1 was identified as coumarin by its spectroscopic data. Both cinnamon water extracts and coumarin inhibit the proliferation of A2780 cells in vitro with 50% inhibitory concentration values of 9.1 and 0.64 mg/mL, respectively. The results showed coumarin alone manifested higher growth inhibition than cinnamon water extract toward A2780 cells. The smaller inhibitory effect of cinnamon water extract can be attributed to the presence of a lower concentration of coumarin in the cinnamon water extract and anti-proliferative property in cinnamon due to coumarin presence may be owing to the synergistic effect of the other compound in cinnamon Colegio de Farmaceuticos de la Provincia de Buenos Aires 2014 Article PeerReviewed Ahmad, Rabiatul Adawiyah and Sayid Abdullah, Sharifah H. A. and Serati-Nouri, Hamed and Abd. Majid, Fadzilah Adibah and Sarmidi, Mohamad Roji and Abdul Aziz, Ramlan (2014) Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells. Latin American Journal of Pharmacy, 33 (6). pp. 960-965. ISSN 0326-2383 http://www.latamjpharm.org/
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ahmad, Rabiatul Adawiyah Sayid Abdullah, Sharifah H. A. Serati-Nouri, Hamed Abd. Majid, Fadzilah Adibah Sarmidi, Mohamad Roji Abdul Aziz, Ramlan Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells
description	Coumarin is one of the important compounds of cinnamon which possess many biological activities such as anticoagulation, antithrombotic, antimicrobial and anti-oxidant activities. This study was performed to demonstrate the presence of coumarin in cinnamon stem bark through chromatographic fractionation and exploring the potential anticancer properties of cinnamon extract on human ovarian epithelial carcinoma cell line A2780. Water and solvent extraction, chromatographic fractionation and isolation were done for dried cinnamon. The structure of the isolated compound 1 was elucidated by spectroscopic methods. Separation and quantification of compound 1 in cinnamon water extracts were carried out by HPLC. Identification of the compound 1 was achieved by comparing retention times and UV spectra with the standard curve. Cytotoxicity analysis was performed using MTT assay in which A2780 cells were exposed to different concentrations of cinnamon water extract and compound 1. Compound 1 was identified as coumarin by its spectroscopic data. Both cinnamon water extracts and coumarin inhibit the proliferation of A2780 cells in vitro with 50% inhibitory concentration values of 9.1 and 0.64 mg/mL, respectively. The results showed coumarin alone manifested higher growth inhibition than cinnamon water extract toward A2780 cells. The smaller inhibitory effect of cinnamon water extract can be attributed to the presence of a lower concentration of coumarin in the cinnamon water extract and anti-proliferative property in cinnamon due to coumarin presence may be owing to the synergistic effect of the other compound in cinnamon
format	Article
author	Ahmad, Rabiatul Adawiyah Sayid Abdullah, Sharifah H. A. Serati-Nouri, Hamed Abd. Majid, Fadzilah Adibah Sarmidi, Mohamad Roji Abdul Aziz, Ramlan
author_facet	Ahmad, Rabiatul Adawiyah Sayid Abdullah, Sharifah H. A. Serati-Nouri, Hamed Abd. Majid, Fadzilah Adibah Sarmidi, Mohamad Roji Abdul Aziz, Ramlan
author_sort	Ahmad, Rabiatul Adawiyah
title	Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells
title_short	Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells
title_full	Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells
title_fullStr	Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells
title_full_unstemmed	Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells
title_sort	antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells
publisher	Colegio de Farmaceuticos de la Provincia de Buenos Aires
publishDate	2014
url	http://eprints.utm.my/id/eprint/51879/ http://www.latamjpharm.org/
_version_	1643653088714686464
score	13.211869

Antiproliferative activity of coumarin and cinnamon water extracts on human ovarian cancer cells

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