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Effect of agarose on viability of seeded cells

Agarose hydrogel is commonly used in tissue engineering studies to provide three dimensional environment for the cells to grow. This research was undertaken to study the effect of different concentrations of agarose gels on the viability of D11 insulin cells and thus evaluating agarose biodegradabil...

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Main Author: Hassan, Mozan Osman
Format: Thesis
Language:English
Published: 2014
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/48010/25/MozanOsmanHassanMFBME2014.pdf
http://eprints.utm.my/id/eprint/48010/
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spelling my.utm.480102017-08-11T06:06:54Z http://eprints.utm.my/id/eprint/48010/ Effect of agarose on viability of seeded cells Hassan, Mozan Osman Q Science (General) Agarose hydrogel is commonly used in tissue engineering studies to provide three dimensional environment for the cells to grow. This research was undertaken to study the effect of different concentrations of agarose gels on the viability of D11 insulin cells and thus evaluating agarose biodegradability and biocompatibility. The degradation of different concentrations of agarose 1, 2 and 3% in RPMI media were observed and the weight loss and degradation rate were measured for 14 days. To test agarose biocompatibility on D11 cells and optimize the seeding density, different concentrations of agarose (1, 2 and 3%) gel were seeded with 4 different seeding densities and the viability of the seeded cells were observed for 7 days (day 2, 4 and 7) by using MTT assay. This was done to see the effect of different agarose concentrations and different seeding densities on cells viability. Results for degradation study showed the degradation rate of agarose was relatively slow for all concentrations, and the weight loss in 1% agarose was the highest indicating that its degrading faster followed by 2% and 3% respectively. Results for MTT assay showed differences in cells viability with different agarose concentrations and different seeding densities. Cells viability found to be decreased at higher agarose concentrations, while lower agarose concentrations as 1% enhanced cell viability the best. Higher seeding densities as 1×106cell/ml found to be more suitable for seeding on agarose and showed the highest percentage viability (˜50%) at low agarose concentrations. This can be concluded that agarose hydrogel is suitable and a compatible material for 3 dimension culture of D11 cells and that by altering agarose concentration and seeding density cell proliferation in agarose can be controlled. 2014-05 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/48010/25/MozanOsmanHassanMFBME2014.pdf Hassan, Mozan Osman (2014) Effect of agarose on viability of seeded cells. Masters thesis, Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Hassan, Mozan Osman
Effect of agarose on viability of seeded cells
description Agarose hydrogel is commonly used in tissue engineering studies to provide three dimensional environment for the cells to grow. This research was undertaken to study the effect of different concentrations of agarose gels on the viability of D11 insulin cells and thus evaluating agarose biodegradability and biocompatibility. The degradation of different concentrations of agarose 1, 2 and 3% in RPMI media were observed and the weight loss and degradation rate were measured for 14 days. To test agarose biocompatibility on D11 cells and optimize the seeding density, different concentrations of agarose (1, 2 and 3%) gel were seeded with 4 different seeding densities and the viability of the seeded cells were observed for 7 days (day 2, 4 and 7) by using MTT assay. This was done to see the effect of different agarose concentrations and different seeding densities on cells viability. Results for degradation study showed the degradation rate of agarose was relatively slow for all concentrations, and the weight loss in 1% agarose was the highest indicating that its degrading faster followed by 2% and 3% respectively. Results for MTT assay showed differences in cells viability with different agarose concentrations and different seeding densities. Cells viability found to be decreased at higher agarose concentrations, while lower agarose concentrations as 1% enhanced cell viability the best. Higher seeding densities as 1×106cell/ml found to be more suitable for seeding on agarose and showed the highest percentage viability (˜50%) at low agarose concentrations. This can be concluded that agarose hydrogel is suitable and a compatible material for 3 dimension culture of D11 cells and that by altering agarose concentration and seeding density cell proliferation in agarose can be controlled.
format Thesis
author Hassan, Mozan Osman
author_facet Hassan, Mozan Osman
author_sort Hassan, Mozan Osman
title Effect of agarose on viability of seeded cells
title_short Effect of agarose on viability of seeded cells
title_full Effect of agarose on viability of seeded cells
title_fullStr Effect of agarose on viability of seeded cells
title_full_unstemmed Effect of agarose on viability of seeded cells
title_sort effect of agarose on viability of seeded cells
publishDate 2014
url http://eprints.utm.my/id/eprint/48010/25/MozanOsmanHassanMFBME2014.pdf
http://eprints.utm.my/id/eprint/48010/
_version_ 1643652432365879296
score 13.211869

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