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Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM

We performed single pulses current measurement on single cells using dual nanoprobe through environmental scanning electron microscope nanomanipulator system. The ability to characterize the electrical property of single cells can be used as a novel method for cell viability detection in quantitativ...

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Main Authors:	Ahmad, Mohd. Ridzuan, Nakajima, Masahiro, Kojima, Masaru, Kojima, Seiji, Homma, Michio, Fukuda, Toshio
Format:	Article
Published:	IEEE 2012
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://eprints.utm.my/id/eprint/47110/ http://dx.doi.org/10.1109/TNANO.2011.2171989
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id	my.utm.47110
record_format	eprints
spelling	my.utm.471102019-03-31T08:31:51Z http://eprints.utm.my/id/eprint/47110/ Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM Ahmad, Mohd. Ridzuan Nakajima, Masahiro Kojima, Masaru Kojima, Seiji Homma, Michio Fukuda, Toshio TA Engineering (General). Civil engineering (General) We performed single pulses current measurement on single cells using dual nanoprobe through environmental scanning electron microscope nanomanipulator system. The ability to characterize the electrical property of single cells can be used as a novel method for cell viability detection in quantitative and instantaneous manners. The nanoprobe was successfully fabricated using focused ion beam tungsten deposition and etching processes. The characteristics of the nanoprobe were examined from the energy dispersion spectrometry and noise analyses. In this paper, for the first time, the electrical property of single cells under their native condition was presented. In order to apply this method for cell viability detection, two types of cells were used, i.e., dead cells and live cells. The results showed that there is a significant difference on the electrical measurement data between dead and live cells. IEEE 2012 Article PeerReviewed Ahmad, Mohd. Ridzuan and Nakajima, Masahiro and Kojima, Masaru and Kojima, Seiji and Homma, Michio and Fukuda, Toshio (2012) Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM. IEEE Transactions on Nanotechnology, 11 (2). pp. 298-306. ISSN 1536-125X http://dx.doi.org/10.1109/TNANO.2011.2171989 DOI:10.1109/TNANO.2011.2171989
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Ahmad, Mohd. Ridzuan Nakajima, Masahiro Kojima, Masaru Kojima, Seiji Homma, Michio Fukuda, Toshio Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM
description	We performed single pulses current measurement on single cells using dual nanoprobe through environmental scanning electron microscope nanomanipulator system. The ability to characterize the electrical property of single cells can be used as a novel method for cell viability detection in quantitative and instantaneous manners. The nanoprobe was successfully fabricated using focused ion beam tungsten deposition and etching processes. The characteristics of the nanoprobe were examined from the energy dispersion spectrometry and noise analyses. In this paper, for the first time, the electrical property of single cells under their native condition was presented. In order to apply this method for cell viability detection, two types of cells were used, i.e., dead cells and live cells. The results showed that there is a significant difference on the electrical measurement data between dead and live cells.
format	Article
author	Ahmad, Mohd. Ridzuan Nakajima, Masahiro Kojima, Masaru Kojima, Seiji Homma, Michio Fukuda, Toshio
author_facet	Ahmad, Mohd. Ridzuan Nakajima, Masahiro Kojima, Masaru Kojima, Seiji Homma, Michio Fukuda, Toshio
author_sort	Ahmad, Mohd. Ridzuan
title	Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM
title_short	Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM
title_full	Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM
title_fullStr	Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM
title_full_unstemmed	Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM
title_sort	instantaneous and quantitave single cells viability determination using dual nanopobe inside esem
publisher	IEEE
publishDate	2012
url	http://eprints.utm.my/id/eprint/47110/ http://dx.doi.org/10.1109/TNANO.2011.2171989
_version_	1643652236646023168
score	13.211869

Instantaneous and quantitave single cells viability determination using dual nanopobe inside ESEM

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