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Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds

For bone tissue regeneration, composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramics have been regarded as promising biomimetic systems. Polymer blends of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) can be used as the polyme...

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Main Authors: Sultana, Naznin, Khan, Tareef Hayat
Format: Article
Published: 2012
Subjects:
TA Engineering (General). Civil engineering (General)
Online Access:http://eprints.utm.my/id/eprint/46971/
http://dx.doi.org/10.1155/2012/656914
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spelling my.utm.469712017-09-27T08:01:13Z http://eprints.utm.my/id/eprint/46971/ Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds Sultana, Naznin Khan, Tareef Hayat TA Engineering (General). Civil engineering (General) For bone tissue regeneration, composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramics have been regarded as promising biomimetic systems. Polymer blends of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) can be used as the polymer matrix to control the degradation rate. In order to render the scaffolds osteoconductive, nano-sized hydroxyapatite (nHA) particles can be incorporated into the polymer matrix. In the first part of this study, a factorial design approach to investigate the influence of materials on the initial compressive mechanical properties of the scaffolds was studied. In the second part, the protein adsorption behavior and the attachment and morphology of osteoblast-like cells (Saos-2) of the scaffolds in vitro were also studied. It was observed that nHA incorporated PHBV/PLLA composite scaffolds adsorbed more bovine serum albumin (BSA) protein than PHBV or PHBV/PLLA scaffolds. In vitro studies also revealed that the attachment of human osteoblastic cells (SaOS-2) was significantly higher in nHA incorporated PHBV/PLLA composite scaffolds. From the SEM micrographs of nHA incorporated PHBV/PLLA composite scaffolds seeded with SaOS-2 cells after a 7-day cell culture period, it was observed that the cells were well expanded and spread in all directions on the scaffolds 2012 Article PeerReviewed Sultana, Naznin and Khan, Tareef Hayat (2012) Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds. Journal of Nanomaterials . pp. 1-8. ISSN 1687-4110 http://dx.doi.org/10.1155/2012/656914
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TA Engineering (General). Civil engineering (General)
spellingShingle TA Engineering (General). Civil engineering (General)
Sultana, Naznin
Khan, Tareef Hayat
Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds
description For bone tissue regeneration, composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramics have been regarded as promising biomimetic systems. Polymer blends of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) can be used as the polymer matrix to control the degradation rate. In order to render the scaffolds osteoconductive, nano-sized hydroxyapatite (nHA) particles can be incorporated into the polymer matrix. In the first part of this study, a factorial design approach to investigate the influence of materials on the initial compressive mechanical properties of the scaffolds was studied. In the second part, the protein adsorption behavior and the attachment and morphology of osteoblast-like cells (Saos-2) of the scaffolds in vitro were also studied. It was observed that nHA incorporated PHBV/PLLA composite scaffolds adsorbed more bovine serum albumin (BSA) protein than PHBV or PHBV/PLLA scaffolds. In vitro studies also revealed that the attachment of human osteoblastic cells (SaOS-2) was significantly higher in nHA incorporated PHBV/PLLA composite scaffolds. From the SEM micrographs of nHA incorporated PHBV/PLLA composite scaffolds seeded with SaOS-2 cells after a 7-day cell culture period, it was observed that the cells were well expanded and spread in all directions on the scaffolds
format Article
author Sultana, Naznin
Khan, Tareef Hayat
author_facet Sultana, Naznin
Khan, Tareef Hayat
author_sort Sultana, Naznin
title Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds
title_short Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds
title_full Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds
title_fullStr Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds
title_full_unstemmed Factorial study of compressive mechanical properties and primary in vitro osteoblast response of PHBV/PILA scaffolds
title_sort factorial study of compressive mechanical properties and primary in vitro osteoblast response of phbv/pila scaffolds
publishDate 2012
url http://eprints.utm.my/id/eprint/46971/
http://dx.doi.org/10.1155/2012/656914
_version_ 1643652195989585920
score 13.211869

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