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Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133

A white-rot fungus of Polyporus sp. S133 was isolated from an oil-polluted soil. The metabolism of pyrene by this fungus was investigated in liquid medium with 5 mg of the compound. Depletion of pyrene was evident during the 30-day growth period and was 21% and 90%, respectively, in cometabolism and...

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Main Authors: Hadibarata, Tony, Kristanti, Risky Ayu, Fulazzaky, Mohamad Ali, Nugroho, Agung Endro
Format: Article
Published: 2012
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/46686/
http://dx.doi.org/0.1002/bab.1048
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spelling my.utm.466862017-09-18T03:42:21Z http://eprints.utm.my/id/eprint/46686/ Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133 Hadibarata, Tony Kristanti, Risky Ayu Fulazzaky, Mohamad Ali Nugroho, Agung Endro TP Chemical technology A white-rot fungus of Polyporus sp. S133 was isolated from an oil-polluted soil. The metabolism of pyrene by this fungus was investigated in liquid medium with 5 mg of the compound. Depletion of pyrene was evident during the 30-day growth period and was 21% and 90%, respectively, in cometabolism and metabolism of pyrene alone. Pyrene was absorbed to fungal cells or biodegraded to form simpler structural compounds. Seventy-one percent of eliminated pyrene was transformed by Polyporus sp. S133 into other compounds, whereas only 18% was absorbed in the fungal cell. The effects of pH and temperature on biomass production of Polyporus sp. S133 for pyrene were examined; the properties of laccase and 1,2-dioxygenase produced by Polyporus sp. S133 during pyrene degradation were investigated. The optimal values of pH were 3, 5, and 4 for laccase, 1,2-dioxygenase, and biomass production, respectively, whereas the optimal values of temperature were 25 °C for laccase and 50 °C for 1,2-dioxygenase and biomass production. Under optimal conditions, pyrene was mainly metabolized to 1-hydroxypyrene and gentisic acid. The structure of 1-hydroxypyrene and gentisic acid was determined by gas chromatography-mass spectrometry after identification using thin-layer chromatography. 2012 Article PeerReviewed Hadibarata, Tony and Kristanti, Risky Ayu and Fulazzaky, Mohamad Ali and Nugroho, Agung Endro (2012) Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133. Biotechnology and Applied Biochemistry, 59 (6). pp. 465-470. ISSN 0885-4513 http://dx.doi.org/0.1002/bab.1048 doi.org/10.1002/bab.1048
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Hadibarata, Tony
Kristanti, Risky Ayu
Fulazzaky, Mohamad Ali
Nugroho, Agung Endro
Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133
description A white-rot fungus of Polyporus sp. S133 was isolated from an oil-polluted soil. The metabolism of pyrene by this fungus was investigated in liquid medium with 5 mg of the compound. Depletion of pyrene was evident during the 30-day growth period and was 21% and 90%, respectively, in cometabolism and metabolism of pyrene alone. Pyrene was absorbed to fungal cells or biodegraded to form simpler structural compounds. Seventy-one percent of eliminated pyrene was transformed by Polyporus sp. S133 into other compounds, whereas only 18% was absorbed in the fungal cell. The effects of pH and temperature on biomass production of Polyporus sp. S133 for pyrene were examined; the properties of laccase and 1,2-dioxygenase produced by Polyporus sp. S133 during pyrene degradation were investigated. The optimal values of pH were 3, 5, and 4 for laccase, 1,2-dioxygenase, and biomass production, respectively, whereas the optimal values of temperature were 25 °C for laccase and 50 °C for 1,2-dioxygenase and biomass production. Under optimal conditions, pyrene was mainly metabolized to 1-hydroxypyrene and gentisic acid. The structure of 1-hydroxypyrene and gentisic acid was determined by gas chromatography-mass spectrometry after identification using thin-layer chromatography.
format Article
author Hadibarata, Tony
Kristanti, Risky Ayu
Fulazzaky, Mohamad Ali
Nugroho, Agung Endro
author_facet Hadibarata, Tony
Kristanti, Risky Ayu
Fulazzaky, Mohamad Ali
Nugroho, Agung Endro
author_sort Hadibarata, Tony
title Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133
title_short Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133
title_full Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133
title_fullStr Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133
title_full_unstemmed Characterization of pyrene biodegradation by white-rot fungus polyporus SP. S133
title_sort characterization of pyrene biodegradation by white-rot fungus polyporus sp. s133
publishDate 2012
url http://eprints.utm.my/id/eprint/46686/
http://dx.doi.org/0.1002/bab.1048
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score 13.211869

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