Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation

The potential use of Bacillus licheniformis coated bioparticles for hydrogen peroxide (H2O2) degradation was assessed in this study. Bioparticles were made by mixing zeolite, activated carbon and cement in ratio 20:5:6 for attachment of biofilm. The efficiency of H2O2 degradation was examined in the...

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Main Authors: Teoh, Wei Kheng, Ibrahim, Zaharah, Shahir, Shafinaz
Format: Article
Published: Penerbit UTM Press 2012
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Online Access:http://eprints.utm.my/id/eprint/46634/
http://dx.doi.org/10.11113/jt.v59.1570
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spelling my.utm.466342017-11-01T04:17:14Z http://eprints.utm.my/id/eprint/46634/ Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation Teoh, Wei Kheng Ibrahim, Zaharah Shahir, Shafinaz TK Electrical engineering. Electronics Nuclear engineering The potential use of Bacillus licheniformis coated bioparticles for hydrogen peroxide (H2O2) degradation was assessed in this study. Bioparticles were made by mixing zeolite, activated carbon and cement in ratio 20:5:6 for attachment of biofilm. The efficiency of H2O2 degradation was examined in the presence and absence of biofilm (control) on bioparticles. Optimisation of biofilm development (7 and 10 days) and reusability were also investigated for H2O2degradation. Actively growing bacterial suspension (late exponential phase) of B.licheniformis was used in development of pure culture biofilm. The 7–day biofilm coated bioparticles system successfully achieved complete H2O2 degradation within an hour (highest rate = 1.17 % H2O2 degraded per minute) while the control showed no significant H2O2 degradation. After repeated use of biofilm coated bioparticles, the rate of H2O2 degradation declined to 0.654 % H2O2degraded per minute, and second use, the rate of H2O2 degradation was 0.166 % H2O2 degraded per minute. Field Emission Scanning Electron Microscope (FESEM) images of the biofilm coated bioparticles showed the attachment of cells and formation of extracellular polymeric substances (EPS), whereas the control showed no biofilm formed. Penerbit UTM Press 2012 Article PeerReviewed Teoh, Wei Kheng and Ibrahim, Zaharah and Shahir, Shafinaz (2012) Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation. Jurnal Teknologi (Sciences and Engineering), 59 (SUP. 1). pp. 1-9. ISSN 0127-9696 http://dx.doi.org/10.11113/jt.v59.1570
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TK Electrical engineering. Electronics Nuclear engineering
spellingShingle TK Electrical engineering. Electronics Nuclear engineering
Teoh, Wei Kheng
Ibrahim, Zaharah
Shahir, Shafinaz
Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation
description The potential use of Bacillus licheniformis coated bioparticles for hydrogen peroxide (H2O2) degradation was assessed in this study. Bioparticles were made by mixing zeolite, activated carbon and cement in ratio 20:5:6 for attachment of biofilm. The efficiency of H2O2 degradation was examined in the presence and absence of biofilm (control) on bioparticles. Optimisation of biofilm development (7 and 10 days) and reusability were also investigated for H2O2degradation. Actively growing bacterial suspension (late exponential phase) of B.licheniformis was used in development of pure culture biofilm. The 7–day biofilm coated bioparticles system successfully achieved complete H2O2 degradation within an hour (highest rate = 1.17 % H2O2 degraded per minute) while the control showed no significant H2O2 degradation. After repeated use of biofilm coated bioparticles, the rate of H2O2 degradation declined to 0.654 % H2O2degraded per minute, and second use, the rate of H2O2 degradation was 0.166 % H2O2 degraded per minute. Field Emission Scanning Electron Microscope (FESEM) images of the biofilm coated bioparticles showed the attachment of cells and formation of extracellular polymeric substances (EPS), whereas the control showed no biofilm formed.
format Article
author Teoh, Wei Kheng
Ibrahim, Zaharah
Shahir, Shafinaz
author_facet Teoh, Wei Kheng
Ibrahim, Zaharah
Shahir, Shafinaz
author_sort Teoh, Wei Kheng
title Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation
title_short Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation
title_full Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation
title_fullStr Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation
title_full_unstemmed Bacillus licheniformis coated bioparticles for hydrogen peroxide degradation
title_sort bacillus licheniformis coated bioparticles for hydrogen peroxide degradation
publisher Penerbit UTM Press
publishDate 2012
url http://eprints.utm.my/id/eprint/46634/
http://dx.doi.org/10.11113/jt.v59.1570
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score 13.211869