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A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses

SAG21/AtLEA5 belongs to the late embryogenesis-associated (LEA) protein family. Although it has been implicated in growth and redox responses, its precise roles remain obscure. To address this problem, we characterized root and shoot development and response to biotic stress in SAG21/AtLEA5 over-exp...

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Main Authors: Mohd. Salleh, Faezah, Evans, Karly, Goodall, Benjamin, Machin, Helena, B. Mowla, Shaheen, J. Mur, Luis A., Runions, John, Theodoulou, Frederica L., H. Foyer, Christine, J. Rogers, Hilary
Format: Article
Published: 2012
Subjects:
QK Botany
Online Access:http://eprints.utm.my/id/eprint/46505/
http://dx.doi.org/10.1111/j.1365-3040.2011.02394.x
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spelling my.utm.465052017-09-12T04:41:41Z http://eprints.utm.my/id/eprint/46505/ A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses Mohd. Salleh, Faezah Evans, Karly Goodall, Benjamin Machin, Helena B. Mowla, Shaheen J. Mur, Luis A. Runions, John Theodoulou, Frederica L. H. Foyer, Christine J. Rogers, Hilary QK Botany SAG21/AtLEA5 belongs to the late embryogenesis-associated (LEA) protein family. Although it has been implicated in growth and redox responses, its precise roles remain obscure. To address this problem, we characterized root and shoot development and response to biotic stress in SAG21/AtLEA5 over-expressor (OEX) and antisense (AS) lines. AS lines exhibited earlier flowering and senescence and reduced shoot biomass. Primary root length was reduced in AS lines, as was the number of laterals relative to the primary root. Root hair number was unchanged but root hair length was proportional to SAG21/AtLEA5 expression level, with longer root hairs in OEX lines and shorter root hairs in AS, relative to wild type. Growth of the fungal nectroph, Botrytis cinerea and of a virulent bacterial pathogen (Pseudomonas syringae pv. tomato) was affected by SAG21/AtLEA5 expression; however, growth of an avirulent P.syringae strain was unaffected. A SAG21/AtLEA5-YFP fusion was localized to mitochondria, raising the intriguing possibility that SAG21 interacts with proteins involved in mitochondrial ROS signalling, which in turn, impacts on root development and pathogen responses. 2012 Article PeerReviewed Mohd. Salleh, Faezah and Evans, Karly and Goodall, Benjamin and Machin, Helena and B. Mowla, Shaheen and J. Mur, Luis A. and Runions, John and Theodoulou, Frederica L. and H. Foyer, Christine and J. Rogers, Hilary (2012) A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses. Plant Cell and Environment, 35 (2). pp. 418-429. ISSN 0140-7791 http://dx.doi.org/10.1111/j.1365-3040.2011.02394.x
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QK Botany
spellingShingle QK Botany
Mohd. Salleh, Faezah
Evans, Karly
Goodall, Benjamin
Machin, Helena
B. Mowla, Shaheen
J. Mur, Luis A.
Runions, John
Theodoulou, Frederica L.
H. Foyer, Christine
J. Rogers, Hilary
A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses
description SAG21/AtLEA5 belongs to the late embryogenesis-associated (LEA) protein family. Although it has been implicated in growth and redox responses, its precise roles remain obscure. To address this problem, we characterized root and shoot development and response to biotic stress in SAG21/AtLEA5 over-expressor (OEX) and antisense (AS) lines. AS lines exhibited earlier flowering and senescence and reduced shoot biomass. Primary root length was reduced in AS lines, as was the number of laterals relative to the primary root. Root hair number was unchanged but root hair length was proportional to SAG21/AtLEA5 expression level, with longer root hairs in OEX lines and shorter root hairs in AS, relative to wild type. Growth of the fungal nectroph, Botrytis cinerea and of a virulent bacterial pathogen (Pseudomonas syringae pv. tomato) was affected by SAG21/AtLEA5 expression; however, growth of an avirulent P.syringae strain was unaffected. A SAG21/AtLEA5-YFP fusion was localized to mitochondria, raising the intriguing possibility that SAG21 interacts with proteins involved in mitochondrial ROS signalling, which in turn, impacts on root development and pathogen responses.
format Article
author Mohd. Salleh, Faezah
Evans, Karly
Goodall, Benjamin
Machin, Helena
B. Mowla, Shaheen
J. Mur, Luis A.
Runions, John
Theodoulou, Frederica L.
H. Foyer, Christine
J. Rogers, Hilary
author_facet Mohd. Salleh, Faezah
Evans, Karly
Goodall, Benjamin
Machin, Helena
B. Mowla, Shaheen
J. Mur, Luis A.
Runions, John
Theodoulou, Frederica L.
H. Foyer, Christine
J. Rogers, Hilary
author_sort Mohd. Salleh, Faezah
title A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses
title_short A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses
title_full A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses
title_fullStr A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses
title_full_unstemmed A novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses
title_sort novel function for a redox-related lea protein (sag21/atlea5) in root development and biotic stress responses
publishDate 2012
url http://eprints.utm.my/id/eprint/46505/
http://dx.doi.org/10.1111/j.1365-3040.2011.02394.x
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score 13.211869

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