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Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis

Random Amplified Polymorphic DNA (RAPD) analysis was done on Eurycoma Longifolia Jack (ELJ) cultivars originating from 7 different locations in Malaysia to determine whether the originality of ELJ cultivars can be preserved in an uncontrolled cultivation area. Out of 60 arbitrary 10-mer primers, a t...

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Main Authors: Mohd. Razi, Aishatur Radhiah, Abd. Aziz, Azila, Syed Alwee, Sharifah Shahrul Bariah, Aziz, Ramlan
Format: Article
Published: Lifescience Global 2013
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/40755/
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spelling my.utm.407552017-02-15T06:36:53Z http://eprints.utm.my/id/eprint/40755/ Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis Mohd. Razi, Aishatur Radhiah Abd. Aziz, Azila Syed Alwee, Sharifah Shahrul Bariah Aziz, Ramlan QD Chemistry Random Amplified Polymorphic DNA (RAPD) analysis was done on Eurycoma Longifolia Jack (ELJ) cultivars originating from 7 different locations in Malaysia to determine whether the originality of ELJ cultivars can be preserved in an uncontrolled cultivation area. Out of 60 arbitrary 10-mer primers, a total of 320 DNA fragments were amplified with an average of 53.3 RAPD markers per primer. 71 (22%) of the 320 DNA-fragments was found to be monomorphic. The remaining 249 (78%) were polymorphic. The approximate size of the largest fragment produced was 3000 base pair (bp) and the easily recognizable fragment produced was 300 bp. Similarity index of RAPD banding patterns was used to investigate the relationships between ELJ cultivars. Mantel test showed that the correlation between Jaccard and Dice similarity matrices was high and significant (0.9907). The dendogram showed that ELJ cultivars can be clustered into three main clusters. Cluster 1 consisted of Sabah cultivars (East Malaysia), Cluster 2 consisted of Pahang and Terengganu cultivars (middle part of West Malaysia) while cluster 3 consisted of Kedah and Kelantan cultivars (east part of West Malaysia). The results of Principle Coordinates Analysis (PCA) were comparable to the Dendogram by UPGMA. The first two principle coordinates explained 53.62% of total variations and separated the 37 ELJ cultivars into three main clusters with a slight overlap between each other. Lifescience Global 2013 Article PeerReviewed Mohd. Razi, Aishatur Radhiah and Abd. Aziz, Azila and Syed Alwee, Sharifah Shahrul Bariah and Aziz, Ramlan (2013) Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis. International Journal of Biotechnology for Wellness Industries, 2 (n/a). pp. 45-50. ISSN 1927-3037/13
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
spellingShingle QD Chemistry
Mohd. Razi, Aishatur Radhiah
Abd. Aziz, Azila
Syed Alwee, Sharifah Shahrul Bariah
Aziz, Ramlan
Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis
description Random Amplified Polymorphic DNA (RAPD) analysis was done on Eurycoma Longifolia Jack (ELJ) cultivars originating from 7 different locations in Malaysia to determine whether the originality of ELJ cultivars can be preserved in an uncontrolled cultivation area. Out of 60 arbitrary 10-mer primers, a total of 320 DNA fragments were amplified with an average of 53.3 RAPD markers per primer. 71 (22%) of the 320 DNA-fragments was found to be monomorphic. The remaining 249 (78%) were polymorphic. The approximate size of the largest fragment produced was 3000 base pair (bp) and the easily recognizable fragment produced was 300 bp. Similarity index of RAPD banding patterns was used to investigate the relationships between ELJ cultivars. Mantel test showed that the correlation between Jaccard and Dice similarity matrices was high and significant (0.9907). The dendogram showed that ELJ cultivars can be clustered into three main clusters. Cluster 1 consisted of Sabah cultivars (East Malaysia), Cluster 2 consisted of Pahang and Terengganu cultivars (middle part of West Malaysia) while cluster 3 consisted of Kedah and Kelantan cultivars (east part of West Malaysia). The results of Principle Coordinates Analysis (PCA) were comparable to the Dendogram by UPGMA. The first two principle coordinates explained 53.62% of total variations and separated the 37 ELJ cultivars into three main clusters with a slight overlap between each other.
format Article
author Mohd. Razi, Aishatur Radhiah
Abd. Aziz, Azila
Syed Alwee, Sharifah Shahrul Bariah
Aziz, Ramlan
author_facet Mohd. Razi, Aishatur Radhiah
Abd. Aziz, Azila
Syed Alwee, Sharifah Shahrul Bariah
Aziz, Ramlan
author_sort Mohd. Razi, Aishatur Radhiah
title Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis
title_short Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis
title_full Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis
title_fullStr Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis
title_full_unstemmed Relationships between Malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis
title_sort relationships between malaysians cultivars of tongkat ali (eurycoma longifolia jack) obtained through rapd analysis
publisher Lifescience Global
publishDate 2013
url http://eprints.utm.my/id/eprint/40755/
_version_ 1643650547218120704
score 13.244369

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