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Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles

Sample preparation is the most time consuming part of elemental analysis where homogeneous samples that are almost free from organic matter is necessary. Therefore, preliminary operations such as separation of interferents and preconcentration of the analytes are required prior to analysis. Ultrason...

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Main Author: Ahmad, Norazah
Format: Thesis
Language:English
Published: 2013
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/38862/5/NorazahAhmadMFS2013.pdf
http://eprints.utm.my/id/eprint/38862/
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spelling my.utm.388622017-07-25T04:42:02Z http://eprints.utm.my/id/eprint/38862/ Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles Ahmad, Norazah QD Chemistry Sample preparation is the most time consuming part of elemental analysis where homogeneous samples that are almost free from organic matter is necessary. Therefore, preliminary operations such as separation of interferents and preconcentration of the analytes are required prior to analysis. Ultrasonic digestion recently proposed as an efficient method for extraction of trace metals from solid samples. The ultrasonic digestion was optimized by using two parameters whilst another two other parameters were used in enzymatic digestion. Conventional one-variable-at-a-time (OVAT) optimization method was applied to both digestion techniques. Fe and Zn were extracted from the samples and were determined by using flame atomic absorption spectrometry (FAAS).The combination of ultrasonic and enzymatic digestion techniques is known as Ultrasonic-Assisted Enzymatic Digestion (USAED) method. The ultrasonication equipment used was ultrasonic bath. Four parameters studied for optimization were sample mass, sonication time, protease enzyme volume and incubation time. The optimum condition of parameters for sample mass was 0.2 g, sonication time of 30 min whilst for enzyme volume was 1000 µL and for incubation time of 24 hours. For the kinetic study, these reactions had undergone zero order reaction. This USAED method was successfully applied for the digestion and kinetic study of the cockle samples. 2013-01 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/38862/5/NorazahAhmadMFS2013.pdf Ahmad, Norazah (2013) Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles. Masters thesis, Universiti Teknologi Malaysia, Faculty of Science.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Ahmad, Norazah
Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
description Sample preparation is the most time consuming part of elemental analysis where homogeneous samples that are almost free from organic matter is necessary. Therefore, preliminary operations such as separation of interferents and preconcentration of the analytes are required prior to analysis. Ultrasonic digestion recently proposed as an efficient method for extraction of trace metals from solid samples. The ultrasonic digestion was optimized by using two parameters whilst another two other parameters were used in enzymatic digestion. Conventional one-variable-at-a-time (OVAT) optimization method was applied to both digestion techniques. Fe and Zn were extracted from the samples and were determined by using flame atomic absorption spectrometry (FAAS).The combination of ultrasonic and enzymatic digestion techniques is known as Ultrasonic-Assisted Enzymatic Digestion (USAED) method. The ultrasonication equipment used was ultrasonic bath. Four parameters studied for optimization were sample mass, sonication time, protease enzyme volume and incubation time. The optimum condition of parameters for sample mass was 0.2 g, sonication time of 30 min whilst for enzyme volume was 1000 µL and for incubation time of 24 hours. For the kinetic study, these reactions had undergone zero order reaction. This USAED method was successfully applied for the digestion and kinetic study of the cockle samples.
format Thesis
author Ahmad, Norazah
author_facet Ahmad, Norazah
author_sort Ahmad, Norazah
title Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_short Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_full Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_fullStr Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_full_unstemmed Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_sort application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
publishDate 2013
url http://eprints.utm.my/id/eprint/38862/5/NorazahAhmadMFS2013.pdf
http://eprints.utm.my/id/eprint/38862/
_version_ 1643650282966482944
score 13.251813

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