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Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes

In this work, two methods of glucose oxidase (GOD) immobilization for peroxide based glucose biosensor had been investigated. For the first immobilization method, GOD was immobilized in freeze–thawed poly (vinyl alcohol) (PVA) at three different PVA concentrations (5%, 10% and 15% PVA). The higher t...

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Main Authors: Abdul Aziz, Azila, Jusoh, Norhana, Ashari, Mohd. Azizul, Hassan, Norhasliza
Format: Article
Language:English
English
Published: Penerbit UTM Press 2010
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/36675/2/121
http://eprints.utm.my/id/eprint/36675/3/AzilaAbdulAziz2010_ImmobilizationofGlucoseOxidaseinFreeze.pdf
http://eprints.utm.my/id/eprint/36675/
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spelling my.utm.366752017-11-01T04:17:19Z http://eprints.utm.my/id/eprint/36675/ Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes Abdul Aziz, Azila Jusoh, Norhana Ashari, Mohd. Azizul Hassan, Norhasliza TP Chemical technology In this work, two methods of glucose oxidase (GOD) immobilization for peroxide based glucose biosensor had been investigated. For the first immobilization method, GOD was immobilized in freeze–thawed poly (vinyl alcohol) (PVA) at three different PVA concentrations (5%, 10% and 15% PVA). The higher the PVA concentration used for immobilization, the better the retention of the enzyme in the membrane. Nevertheless, higher PVA concentration did not necessarily correlate well with enzyme activity. Freeze–thawed PVA–GOD membranes with 10% PVA showed the highest sensitivity to glucose. The performances of freeze–thawed PVA–GOD membranes with 15% and 5% PVA was comparable. For the second immobilization method, GOD was immobilized within PVA/tetramethoxysilane (TMOS) matrix with different (3–glycidoxypropyldimenthylethoxy) silane (3GPDES) concentrations. For the membranes prepared with 1:1 (TMOS: 3GPDES) volumetric ratio, the percentage of enzyme activity remaining at day 40 was about 51%. Meanwhile, membrane prepared with 1:2 (TMOS: 3GPDES) and 1:3 (TMOS: 3GPDES) were quite stable with 69% and 58% of enzyme activity remaining at day 40, respectively. Overall, the glucose biosensor showed very promising performances. Penerbit UTM Press 2010-05 Article PeerReviewed text/html en http://eprints.utm.my/id/eprint/36675/2/121 application/pdf en http://eprints.utm.my/id/eprint/36675/3/AzilaAbdulAziz2010_ImmobilizationofGlucoseOxidaseinFreeze.pdf Abdul Aziz, Azila and Jusoh, Norhana and Ashari, Mohd. Azizul and Hassan, Norhasliza (2010) Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes. Jurnal Teknologi, 52 . pp. 181-192. ISSN 0127-9696 (Print); 2180-3722 (Electronic) DOI:10.11113/jt.v52.131
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Abdul Aziz, Azila
Jusoh, Norhana
Ashari, Mohd. Azizul
Hassan, Norhasliza
Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes
description In this work, two methods of glucose oxidase (GOD) immobilization for peroxide based glucose biosensor had been investigated. For the first immobilization method, GOD was immobilized in freeze–thawed poly (vinyl alcohol) (PVA) at three different PVA concentrations (5%, 10% and 15% PVA). The higher the PVA concentration used for immobilization, the better the retention of the enzyme in the membrane. Nevertheless, higher PVA concentration did not necessarily correlate well with enzyme activity. Freeze–thawed PVA–GOD membranes with 10% PVA showed the highest sensitivity to glucose. The performances of freeze–thawed PVA–GOD membranes with 15% and 5% PVA was comparable. For the second immobilization method, GOD was immobilized within PVA/tetramethoxysilane (TMOS) matrix with different (3–glycidoxypropyldimenthylethoxy) silane (3GPDES) concentrations. For the membranes prepared with 1:1 (TMOS: 3GPDES) volumetric ratio, the percentage of enzyme activity remaining at day 40 was about 51%. Meanwhile, membrane prepared with 1:2 (TMOS: 3GPDES) and 1:3 (TMOS: 3GPDES) were quite stable with 69% and 58% of enzyme activity remaining at day 40, respectively. Overall, the glucose biosensor showed very promising performances.
format Article
author Abdul Aziz, Azila
Jusoh, Norhana
Ashari, Mohd. Azizul
Hassan, Norhasliza
author_facet Abdul Aziz, Azila
Jusoh, Norhana
Ashari, Mohd. Azizul
Hassan, Norhasliza
author_sort Abdul Aziz, Azila
title Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes
title_short Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes
title_full Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes
title_fullStr Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes
title_full_unstemmed Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes
title_sort immobilization of glucose oxidase in freeze - thawed pva and pva/tmos membranes
publisher Penerbit UTM Press
publishDate 2010
url http://eprints.utm.my/id/eprint/36675/2/121
http://eprints.utm.my/id/eprint/36675/3/AzilaAbdulAziz2010_ImmobilizationofGlucoseOxidaseinFreeze.pdf
http://eprints.utm.my/id/eprint/36675/
_version_ 1643649999063482368
score 13.211869

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