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Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb

The phytochemical investigations of the fruits of Piper retrofractum Vahl. and the stems and leaves of Piper arborescens Roxb. have been carried out. The fruits of Piper retrofractum Vahl. were bought from a spice shop at Pandan, Johor Bahru and the methanolic crude extracts of stems and leaves of P...

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Main Author: Fabarani, Nadya Putri
Format: Thesis
Language:English
Published: 2012
Subjects:
QK Botany
Online Access:http://eprints.utm.my/id/eprint/33395/5/NadyaPutriFabaraniMFS2012.pdf
http://eprints.utm.my/id/eprint/33395/
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spelling my.utm.333952018-04-27T01:26:26Z http://eprints.utm.my/id/eprint/33395/ Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb Fabarani, Nadya Putri QK Botany The phytochemical investigations of the fruits of Piper retrofractum Vahl. and the stems and leaves of Piper arborescens Roxb. have been carried out. The fruits of Piper retrofractum Vahl. were bought from a spice shop at Pandan, Johor Bahru and the methanolic crude extracts of stems and leaves of Piper arborescens Roxb. were obtained from UiTM Sarawak, Malaysia. Samples were extracted with Soxhlet extractor using methanol as the solvent to give the crude extracts. The crude extracts were fractionated using vacuum-liquid chromatography and then purified using several chromatographic techniques. The isolated compounds were identified by analysis of various spectral data using infrared, 1D (1H, 13C and DEPT) and 2D (COSY, and HMBC) nuclear magnetic resonance spectroscopies and mass spectrometry. Seven compounds were isolated from these plants. The purification of methanol crude extract of the fruits of Piper retrofractum Vahl. yielded four compounds identified as piperine, oleic acid, N-isobutyl-2E,4E,14Z-eicosatrienamide and methyl piperate. Another three compounds, characterized as sesartemin, diayangambin and 3-(3,4-dimethoxybenzyl)-4-(3',4',5'-trimethoxybenzyl)- tetrahydrofuran-2-ol had been isolated from the methanol crude extract of the stems of Piper arborescens Roxb. The hydrolysis of piperine was done using potassium hydroxide and ethanol to yield piperic acid. The antibacterial test against Gram positive bacteria (Bascillus subtilis and Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli) were performed on the crude extracts and isolated compounds. The crude extracts and piperine were found to show the strongest inhibition against both Gram positive bacteria with MIC and MBC values of 225 µg/mL. 2012-03 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/33395/5/NadyaPutriFabaraniMFS2012.pdf Fabarani, Nadya Putri (2012) Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb. Masters thesis, Universiti Teknologi Malaysia, Faculty of Science.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QK Botany
spellingShingle QK Botany
Fabarani, Nadya Putri
Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb
description The phytochemical investigations of the fruits of Piper retrofractum Vahl. and the stems and leaves of Piper arborescens Roxb. have been carried out. The fruits of Piper retrofractum Vahl. were bought from a spice shop at Pandan, Johor Bahru and the methanolic crude extracts of stems and leaves of Piper arborescens Roxb. were obtained from UiTM Sarawak, Malaysia. Samples were extracted with Soxhlet extractor using methanol as the solvent to give the crude extracts. The crude extracts were fractionated using vacuum-liquid chromatography and then purified using several chromatographic techniques. The isolated compounds were identified by analysis of various spectral data using infrared, 1D (1H, 13C and DEPT) and 2D (COSY, and HMBC) nuclear magnetic resonance spectroscopies and mass spectrometry. Seven compounds were isolated from these plants. The purification of methanol crude extract of the fruits of Piper retrofractum Vahl. yielded four compounds identified as piperine, oleic acid, N-isobutyl-2E,4E,14Z-eicosatrienamide and methyl piperate. Another three compounds, characterized as sesartemin, diayangambin and 3-(3,4-dimethoxybenzyl)-4-(3',4',5'-trimethoxybenzyl)- tetrahydrofuran-2-ol had been isolated from the methanol crude extract of the stems of Piper arborescens Roxb. The hydrolysis of piperine was done using potassium hydroxide and ethanol to yield piperic acid. The antibacterial test against Gram positive bacteria (Bascillus subtilis and Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli) were performed on the crude extracts and isolated compounds. The crude extracts and piperine were found to show the strongest inhibition against both Gram positive bacteria with MIC and MBC values of 225 µg/mL.
format Thesis
author Fabarani, Nadya Putri
author_facet Fabarani, Nadya Putri
author_sort Fabarani, Nadya Putri
title Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb
title_short Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb
title_full Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb
title_fullStr Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb
title_full_unstemmed Phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb
title_sort phytochemicals and antibacterial activity of piper retrofractum vahl and piper arborescens roxb
publishDate 2012
url http://eprints.utm.my/id/eprint/33395/5/NadyaPutriFabaraniMFS2012.pdf
http://eprints.utm.my/id/eprint/33395/
_version_ 1643649318110887936
score 13.211869

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