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Expression of ron tyrosin kinase receptor on breast cancer cell lines

RON receptor is one member of the MET family of receptor tyrosine kinases. When it is activated by its own ligand, macrophage stimulating protein (MSP), RON initiates a downstream signaling pathways which result of some function in the cell such as dissociation, migration, and invasion. Several RON...

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Main Author: Alzeini, Khaled W. K.
Format: Thesis
Language:English
Published: 2013
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Online Access:http://eprints.utm.my/id/eprint/33162/1/KhaledWKAlzeiniMFBME2013.pdf
http://eprints.utm.my/id/eprint/33162/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69994?queryType=vitalDismax&query=Expression+of+ron+tyrosin+kinase+receptor+on+breast+cancer+cell+lines&public=true
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spelling my.utm.331622017-06-20T00:50:21Z http://eprints.utm.my/id/eprint/33162/ Expression of ron tyrosin kinase receptor on breast cancer cell lines Alzeini, Khaled W. K. Unspecified RON receptor is one member of the MET family of receptor tyrosine kinases. When it is activated by its own ligand, macrophage stimulating protein (MSP), RON initiates a downstream signaling pathways which result of some function in the cell such as dissociation, migration, and invasion. Several RON variants that arise through mRNA splicing or by an alternate translational start site have been identified, some with oncogenic potential. The purpose of the present project was to determine the expression of RON receptor in two breast cancer cell lines since it is the most common distribution cancer in women around the world. MDA-MB-213 and MCF-7 breast cancer cell lines and WRL-68 embryonic liver as a normal cell line were used in this experiment to characterize the expression. The expression of RON receptor on the cell surface, the concentration of mRNA and the RON protein were done by utilizing Immunofluorescent staining (IF), polymerase chain reaction (PCR) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) respectively. Collectively, it was found that RON receptor was overexpressed in these two breast cancer cell lines comparing to the normal cell lines. 2013 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/33162/1/KhaledWKAlzeiniMFBME2013.pdf Alzeini, Khaled W. K. (2013) Expression of ron tyrosin kinase receptor on breast cancer cell lines. Masters thesis, Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69994?queryType=vitalDismax&query=Expression+of+ron+tyrosin+kinase+receptor+on+breast+cancer+cell+lines&public=true
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Unspecified
spellingShingle Unspecified
Alzeini, Khaled W. K.
Expression of ron tyrosin kinase receptor on breast cancer cell lines
description RON receptor is one member of the MET family of receptor tyrosine kinases. When it is activated by its own ligand, macrophage stimulating protein (MSP), RON initiates a downstream signaling pathways which result of some function in the cell such as dissociation, migration, and invasion. Several RON variants that arise through mRNA splicing or by an alternate translational start site have been identified, some with oncogenic potential. The purpose of the present project was to determine the expression of RON receptor in two breast cancer cell lines since it is the most common distribution cancer in women around the world. MDA-MB-213 and MCF-7 breast cancer cell lines and WRL-68 embryonic liver as a normal cell line were used in this experiment to characterize the expression. The expression of RON receptor on the cell surface, the concentration of mRNA and the RON protein were done by utilizing Immunofluorescent staining (IF), polymerase chain reaction (PCR) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) respectively. Collectively, it was found that RON receptor was overexpressed in these two breast cancer cell lines comparing to the normal cell lines.
format Thesis
author Alzeini, Khaled W. K.
author_facet Alzeini, Khaled W. K.
author_sort Alzeini, Khaled W. K.
title Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_short Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_full Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_fullStr Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_full_unstemmed Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_sort expression of ron tyrosin kinase receptor on breast cancer cell lines
publishDate 2013
url http://eprints.utm.my/id/eprint/33162/1/KhaledWKAlzeiniMFBME2013.pdf
http://eprints.utm.my/id/eprint/33162/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69994?queryType=vitalDismax&query=Expression+of+ron+tyrosin+kinase+receptor+on+breast+cancer+cell+lines&public=true
_version_ 1643649249849638912
score 13.211869

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