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Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate

In this study, the effect of pulsed-low intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by 3T3 fibroblasts encapsulated in alginate was evaluated. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content and dimethylamine blue as...

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Main Authors: Mohd. Bohari, Siti Pauliena, Liam, M. Grover, Hukins, David W. L.
Format: Article
Published: SAGE Publications Inc. 2012
Subjects:
Q Science
Online Access:http://eprints.utm.my/id/eprint/31127/
http://journals.sagepub.com/doi/10.1177/2041731412454672
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spelling my.utm.311272018-10-14T07:23:51Z http://eprints.utm.my/id/eprint/31127/ Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate Mohd. Bohari, Siti Pauliena Liam, M. Grover Hukins, David W. L. Q Science In this study, the effect of pulsed-low intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by 3T3 fibroblasts encapsulated in alginate was evaluated. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content and dimethylamine blue assay for glycosaminoglycan content were performed on samples from cell cultures treated with pulsed-low intensity ultrasound and a control group. Pulsed-low intensity ultrasound shows no effect on cell proliferation, while collagen and glycosaminoglycan contents were consistently higher in the samples treated with pulsed-low intensity ultrasound, showing a statistically significant difference (p < 0.05) on day 10. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both groups. These results suggest that pulsed-low intensity ultrasound shows no effect on cell proliferation but has potential for inducing collagen and glycosaminoglycan production in cells cultured in alginate gels. SAGE Publications Inc. 2012 Article PeerReviewed Mohd. Bohari, Siti Pauliena and Liam, M. Grover and Hukins, David W. L. (2012) Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate. Journal of Tissue Engineering, 3 (1). pp. 1-7. ISSN 2041-7314 http://journals.sagepub.com/doi/10.1177/2041731412454672 DOI:10.1177/2041731412454672
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science
spellingShingle Q Science
Mohd. Bohari, Siti Pauliena
Liam, M. Grover
Hukins, David W. L.
Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate
description In this study, the effect of pulsed-low intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by 3T3 fibroblasts encapsulated in alginate was evaluated. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content and dimethylamine blue assay for glycosaminoglycan content were performed on samples from cell cultures treated with pulsed-low intensity ultrasound and a control group. Pulsed-low intensity ultrasound shows no effect on cell proliferation, while collagen and glycosaminoglycan contents were consistently higher in the samples treated with pulsed-low intensity ultrasound, showing a statistically significant difference (p < 0.05) on day 10. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both groups. These results suggest that pulsed-low intensity ultrasound shows no effect on cell proliferation but has potential for inducing collagen and glycosaminoglycan production in cells cultured in alginate gels.
format Article
author Mohd. Bohari, Siti Pauliena
Liam, M. Grover
Hukins, David W. L.
author_facet Mohd. Bohari, Siti Pauliena
Liam, M. Grover
Hukins, David W. L.
author_sort Mohd. Bohari, Siti Pauliena
title Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate
title_short Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate
title_full Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate
title_fullStr Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate
title_full_unstemmed Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate
title_sort pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate
publisher SAGE Publications Inc.
publishDate 2012
url http://eprints.utm.my/id/eprint/31127/
http://journals.sagepub.com/doi/10.1177/2041731412454672
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score 13.211869

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