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Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture

Bioactive compounds, isolated from medicinal mushroom, perform various therapeutic properties. Nowadays, Pleurotus ostreatus is the third largest commercially produced mushroom due to its rich nutrition and medicinal property. Recently, it have been reported that this mushroom can produce highly eff...

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Main Author: Maftoun, Parisa
Format: Thesis
Published: 2012
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/30759/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:73557?queryType=vitalDismax&query=Efficient+exopolysaccharaide+production+by+pleurotus+ostreatus+in+submerged+culture
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spelling my.utm.307592020-08-30T08:11:28Z http://eprints.utm.my/id/eprint/30759/ Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture Maftoun, Parisa TP Chemical technology Bioactive compounds, isolated from medicinal mushroom, perform various therapeutic properties. Nowadays, Pleurotus ostreatus is the third largest commercially produced mushroom due to its rich nutrition and medicinal property. Recently, it have been reported that this mushroom can produce highly efficient immunomodulator exopolysaccharide (pleuran) in both solid state and submerged culture. Production of exopolysaccharide (EPS) in submerged culture not only reduces the production time, but also reduces many downstream steps for polysaccharides extraction and purification. In the present work, production of EPS is conducted during submerged cultivation of P. ostreatus in shake flask and in bioreactor cultures. Also the effect of C:N ratio on EPS production and cell morphology was studied. In shake flask culture, maximal polysaccharide produced was 1.25 g/L after 13 days of cultivation. Morover, it was observed that P. ostratus pellet diameter increased by time. In addition, C:N ratio of 40:1was identified as optimum C:N ratio. After this stage, cultivation process was scaled up to 16-L in situ pilot scale stirred tank bioreactor under controlled and uncontrolled pH conditions. Agitation and aeration in both cultures were 200 rpm and 1 v/v/min, respectively. The maximal cell growth and EPS production of 4.48 g/L and 1.97 g/L was obtained in controlled pH culture, respectively. Furthermore, the effect of different aeration rates ranged between 0.5 up to 2.0 v/v/min was studied. Increaseing the aeration rate resulted in significant increase in cell growth up to 7.1 g/L in 2 v/v/min aerated culture. On the other hand, the maximal volumetric and specific EPS production of about 3.1 g/L and 0.58 g/g, respectively, were obtained in 1.5 v/v/min. aerated culture. These results clearly demonstrate the scalability of pleuran production process in stirred tank bioreactor level using P. ostreatus as producer strain. 2012 Thesis NonPeerReviewed Maftoun, Parisa (2012) Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture. Masters thesis, Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resources Engineering. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:73557?queryType=vitalDismax&query=Efficient+exopolysaccharaide+production+by+pleurotus+ostreatus+in+submerged+culture
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Maftoun, Parisa
Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture
description Bioactive compounds, isolated from medicinal mushroom, perform various therapeutic properties. Nowadays, Pleurotus ostreatus is the third largest commercially produced mushroom due to its rich nutrition and medicinal property. Recently, it have been reported that this mushroom can produce highly efficient immunomodulator exopolysaccharide (pleuran) in both solid state and submerged culture. Production of exopolysaccharide (EPS) in submerged culture not only reduces the production time, but also reduces many downstream steps for polysaccharides extraction and purification. In the present work, production of EPS is conducted during submerged cultivation of P. ostreatus in shake flask and in bioreactor cultures. Also the effect of C:N ratio on EPS production and cell morphology was studied. In shake flask culture, maximal polysaccharide produced was 1.25 g/L after 13 days of cultivation. Morover, it was observed that P. ostratus pellet diameter increased by time. In addition, C:N ratio of 40:1was identified as optimum C:N ratio. After this stage, cultivation process was scaled up to 16-L in situ pilot scale stirred tank bioreactor under controlled and uncontrolled pH conditions. Agitation and aeration in both cultures were 200 rpm and 1 v/v/min, respectively. The maximal cell growth and EPS production of 4.48 g/L and 1.97 g/L was obtained in controlled pH culture, respectively. Furthermore, the effect of different aeration rates ranged between 0.5 up to 2.0 v/v/min was studied. Increaseing the aeration rate resulted in significant increase in cell growth up to 7.1 g/L in 2 v/v/min aerated culture. On the other hand, the maximal volumetric and specific EPS production of about 3.1 g/L and 0.58 g/g, respectively, were obtained in 1.5 v/v/min. aerated culture. These results clearly demonstrate the scalability of pleuran production process in stirred tank bioreactor level using P. ostreatus as producer strain.
format Thesis
author Maftoun, Parisa
author_facet Maftoun, Parisa
author_sort Maftoun, Parisa
title Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture
title_short Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture
title_full Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture
title_fullStr Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture
title_full_unstemmed Efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture
title_sort efficient exopolysaccharaide production by pleurotus ostreatus in submerged culture
publishDate 2012
url http://eprints.utm.my/id/eprint/30759/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:73557?queryType=vitalDismax&query=Efficient+exopolysaccharaide+production+by+pleurotus+ostreatus+in+submerged+culture
_version_ 1677781067454480384
score 13.211869

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