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Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor

Hydrolysis of virgin coconut oil (VCO) had been carried out by using an immobilised lipase from Mucor miehei (Lipozyme) in a water-jacketed batch reactor. The kinetic of the hydrolysis was investigated by varying the parameters such as VCO concentration, enzyme loading, water content, and reaction t...

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Main Authors:	Lee, Suan Chua, Alitabarimansor, Meisam, Chew, Tin Lee, Mat, Ramli
Format:	Article
Published:	Hindawi Publishing Corporation 2012
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/30512/ http://dx.doi.org/10.1155/2012/542589
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id	my.utm.30512
record_format	eprints
spelling	my.utm.305122019-09-22T07:25:20Z http://eprints.utm.my/id/eprint/30512/ Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor Lee, Suan Chua Alitabarimansor, Meisam Chew, Tin Lee Mat, Ramli TP Chemical technology Hydrolysis of virgin coconut oil (VCO) had been carried out by using an immobilised lipase from Mucor miehei (Lipozyme) in a water-jacketed batch reactor. The kinetic of the hydrolysis was investigated by varying the parameters such as VCO concentration, enzyme loading, water content, and reaction temperature. It was found that VCO exhibited substrate inhibition at the concentration more than 40% (v/v). Lipozyme also achieved the highest production of free fatty acids, 4.56mMat 1%(w/v) of enzyme loading. The optimum water content for VCO hydrolysis was 7% (v/v). A relatively high content of water was required because water was one of the reactants in the hydrolysis. The progress curve and the temperature profile of the enzymatic hydrolysis also showed that Lipozyme could be used for free fatty acid production at the temperature up to 50°C. However, the highest initial reaction rate and the highest yield of free fatty acid production were at 45 and 40°C, respectively. A 100 hours of initial reaction time has to be compensated in order to obtain the highest yield of free fatty acid production at 40°C. Hindawi Publishing Corporation 2012 Article PeerReviewed Lee, Suan Chua and Alitabarimansor, Meisam and Chew, Tin Lee and Mat, Ramli (2012) Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor. Enzyme Research, 2012 . pp. 1-5. ISSN 2090-0414 http://dx.doi.org/10.1155/2012/542589 DOI:10.1155/2012/542589
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Lee, Suan Chua Alitabarimansor, Meisam Chew, Tin Lee Mat, Ramli Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor
description	Hydrolysis of virgin coconut oil (VCO) had been carried out by using an immobilised lipase from Mucor miehei (Lipozyme) in a water-jacketed batch reactor. The kinetic of the hydrolysis was investigated by varying the parameters such as VCO concentration, enzyme loading, water content, and reaction temperature. It was found that VCO exhibited substrate inhibition at the concentration more than 40% (v/v). Lipozyme also achieved the highest production of free fatty acids, 4.56mMat 1%(w/v) of enzyme loading. The optimum water content for VCO hydrolysis was 7% (v/v). A relatively high content of water was required because water was one of the reactants in the hydrolysis. The progress curve and the temperature profile of the enzymatic hydrolysis also showed that Lipozyme could be used for free fatty acid production at the temperature up to 50°C. However, the highest initial reaction rate and the highest yield of free fatty acid production were at 45 and 40°C, respectively. A 100 hours of initial reaction time has to be compensated in order to obtain the highest yield of free fatty acid production at 40°C.
format	Article
author	Lee, Suan Chua Alitabarimansor, Meisam Chew, Tin Lee Mat, Ramli
author_facet	Lee, Suan Chua Alitabarimansor, Meisam Chew, Tin Lee Mat, Ramli
author_sort	Lee, Suan Chua
title	Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor
title_short	Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor
title_full	Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor
title_fullStr	Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor
title_full_unstemmed	Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor
title_sort	hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor
publisher	Hindawi Publishing Corporation
publishDate	2012
url	http://eprints.utm.my/id/eprint/30512/ http://dx.doi.org/10.1155/2012/542589
_version_	1646010277679857664
score	13.211869

Hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor

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