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Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design

A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, am...

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Main Authors: Abdul Wahid, Manisya Zauri, Md. Salleh, Madihah, Yusof, Faridah, Abdul Karim, Mohammed Ismail, Alam, Md. Zahangir
Format: Article
Published: Academic Journals 2011
Subjects:
Q Science
Online Access:http://eprints.utm.my/id/eprint/29675/
http://dx.doi.org/10.5897/AJB11.008
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spelling my.utm.296752019-04-25T01:32:33Z http://eprints.utm.my/id/eprint/29675/ Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design Abdul Wahid, Manisya Zauri Md. Salleh, Madihah Yusof, Faridah Abdul Karim, Mohammed Ismail Alam, Md. Zahangir Q Science A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, ammonium sulfate concentration, calcium nitrate concentration, yeast extract concentration, tween 80 concentration, pH, incubation time, initial moisture content, inoculum size and substrate amount. Through analysis of variance (ANOVA), it was found that initial moisture content (p= 0.001), incubation time (p= 0.001), inoculum size (p= 0.032) and ammonium sulfate concentration (p= 0.023) have been recognized as significant factors affecting endoglucanase activity in solid state fermentation (SSF) of oil palm empty fruit bunch (OPEFB) by T. reesei RUT C-30. The model established from the ANOVA analysis have a significant value of P model>F= 0.0008 and R 2 of 0.9132. The preoptimized media showed 2.6 fold increased of endoglucanase activity. Academic Journals 2011-08-22 Article PeerReviewed Abdul Wahid, Manisya Zauri and Md. Salleh, Madihah and Yusof, Faridah and Abdul Karim, Mohammed Ismail and Alam, Md. Zahangir (2011) Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design. African Journal of Biotechnology, 10 (46). pp. 9402-9409. ISSN 1684-5315 http://dx.doi.org/10.5897/AJB11.008 DOI:10.5897/AJB11.008
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science
spellingShingle Q Science
Abdul Wahid, Manisya Zauri
Md. Salleh, Madihah
Yusof, Faridah
Abdul Karim, Mohammed Ismail
Alam, Md. Zahangir
Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
description A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, ammonium sulfate concentration, calcium nitrate concentration, yeast extract concentration, tween 80 concentration, pH, incubation time, initial moisture content, inoculum size and substrate amount. Through analysis of variance (ANOVA), it was found that initial moisture content (p= 0.001), incubation time (p= 0.001), inoculum size (p= 0.032) and ammonium sulfate concentration (p= 0.023) have been recognized as significant factors affecting endoglucanase activity in solid state fermentation (SSF) of oil palm empty fruit bunch (OPEFB) by T. reesei RUT C-30. The model established from the ANOVA analysis have a significant value of P model>F= 0.0008 and R 2 of 0.9132. The preoptimized media showed 2.6 fold increased of endoglucanase activity.
format Article
author Abdul Wahid, Manisya Zauri
Md. Salleh, Madihah
Yusof, Faridah
Abdul Karim, Mohammed Ismail
Alam, Md. Zahangir
author_facet Abdul Wahid, Manisya Zauri
Md. Salleh, Madihah
Yusof, Faridah
Abdul Karim, Mohammed Ismail
Alam, Md. Zahangir
author_sort Abdul Wahid, Manisya Zauri
title Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_short Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_full Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_fullStr Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_full_unstemmed Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_sort factors affecting endoglucanase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using plackett-burman design
publisher Academic Journals
publishDate 2011
url http://eprints.utm.my/id/eprint/29675/
http://dx.doi.org/10.5897/AJB11.008
_version_ 1643648352037896192
score 13.211869

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